------------------- INFORMACIÓN GENERAL ------------------- 1. Título del dataset Influence of Excitation Pulse Duration on the Efficiency of Upconversion Nanoparticle-Based FRET 2. Contacto Nombre: Melle Hernández, Sonia Filiación: Universidad Complutense de Madrid Correo electrónico: smellehe@ucm.es ORCID: 0000-0002-9802-6908 3. Descripción del proyecto We systematically investigate the influence of excitation pulse duration and laser power on the measured FRET efficiency between UCNPs (donors) and Cy3 (acceptor). We use time-resolved UCL measurements and theoretical modeling to reveal that lifetime-based FRET quantification is significantly underestimated and that it changes with the excitation pulse width and power. Specifically, we demonstrate that shorter excitation pulses yield higher apparent FRET efficiencies, while longer pulses or high powers tend to underestimate them. These experimental results are supported by a rate-equation model that captures the dynamic interplay between donor excitation, energy transfer, and relaxation kinetics. 4. Descripción del dataset Dataset of this article includes the experimental data 5. Notas 6. Fecha de depósito de los ficheros 06/05/2025 7. Fecha de creación de los ficheros 8. Idioma Inglés -------------------------- AUTORÍA -------------------------- Nombre: Alejandro Apellidos: Casillas-Rubio Filiación: Universidad Complutense de Madrid, Facultad de Óptica y Optometría Correo electrónico: alejcasi@ucm.es ORCID: 0009-0003-8245-4734 Nombre: Khouloud Apellidos: Hamraoui Filiación: Universidad Complutense de Madrid, Facultad de Farmacia Correo electrónico: ORCID: Nombre: Diego Apellidos: Méndez González Filiación: Universidad Complutense de Madrid, Facultad de Farmacia. Instituto Ramón y Cajal de Investigación Sanitaria Correo electrónico: diegomen@ucm.es ORCID: 0000-0002-5976-1694 Nombre: Marco Apellidos: Laurenti Filiación: Universidad Complutense de Madrid, Facultad de Farmacia. Instituto Ramón y Cajal de Investigación Sanitaria Correo electrónico: marclaur@ucm.es ORCID: 0000-0002-0273-7423 Nombre: Benito Jorge Apellidos: Rubio Retama Filiación: Universidad Complutense de Madrid, Facultad de Farmacia. Instituto Ramón y Cajal de Investigación Sanitaria Correo electrónico: bjrubio@ucm.es ORCID: 0000-0002-1785-5844 Nombre: Óscar Apellidos: Gómez Calderón Filiación: Universidad Complutense de Madrid, Facultad de Óptica y Optometría Correo electrónico: oscargc@ucm.es ORCID:0000-0002-6193-0717 Nombre: Sonia Apellidos: Melle Hernández Filiación: Universidad Complutense de Madrid, Facultad de Óptica y Optometría Correo electrónico: smellehe@ucm.es ORCID:0000-0002-9802-6908 -------------------------- METODOLOGÍA -------------------------- 1. Metolodogía 2. Software -------------------------- PALABRAS CLAVE Y MATERIAS -------------------------- Upconverting nanoparticles, FRET -------------------------- INFORMACIÓN DE PATROCINADORES E IDENTIFICADORES DE PROYECTOS -------------------------- Ministerio de Ciencia, Innovación y Universidades PID2023-150645OA-I00 Ministerio de Ciencia, Innovación y Universidades PID2021-122806OB-I00 Ministerio de Ciencia, Innovación y Universidades PID2021-123318OB-I00 Ministerio de Ciencia, Innovación y Universidades TED2021-132317B-I00 Comunidad de Madrid P2022/BMD-7403 RENIM-CM -------------------------- PUBLICACIONES RELACIONADAS -------------------------- 1. Publicación relacionada Influence of Excitation Pulse Duration on the Efficiency of Upconversion Nanoparticle-Based FRET 2. Dataset relacionado -------------------------- INFORMACIÓN GEOGRÁFICA -------------------------- -------------------------- INFORMACIÓN TEMPORAL -------------------------- -------------------------- FICHEROS -------------------------- 1. Ficheros The .rar files contain the datasets corresponding to the figures presented in the article. Specifically, they are .dat files comprising multiple data columns. The first row of each file specifies the magnitude measured in each column, along with the corresponding unit. Figure1D_core_counts.dat - Number of core nanoparticles versus size Figure1D core_normal.dat - Size distribution of core nanoparticles Figure1D_CS1_counts.dat - Number of CS1 nanoparticles versus size Figure1D_CS1_normal.dat - Size distribution of CS1 nanoparticles Figure1D_CS1_counts.dat - Number of CS2 nanoparticles versus size Figure1D_CS2_normal.dat - Size distribution of CS2 nanoparticles Figure1E_Cy3Absorbance.dat - Absorption spectrum of the Cy3 dye Figure1E_Cy3Fluorescence.dat - Fluorescence spectrum of the Cy3 dye Figure1E_laser.dat - Laser emission spectrum Figure1E_UCLIntensity.dat - UCNPs luminescence spectrum Figura 2A.dat - Green UCL emission spectra for CS1 (with and without Cy3). Figura 2B.dat - Green (540 nm) UCL decay curves for CS1 with and without Cy3 using 2.2W and two excitation pulse widths (40 μs and 10 ms) Figura 2C.dat - Green UCL lifetime as a function of the excitation pulse width for CS1 with and without Cy3. Figura 2D.dat - FRET efficiency calculated from data of figure (C). Figura 2E.dat - Green UCL emission spectra for CS2 (with and without Cy3). Figura 2F.dat - Green (540 nm) UCL decay curves for CS2 with and without Cy3 using 2.2W and two excitation pulse widths (40 μs and 10 ms) Figura 2G.dat - Green UCL lifetime as a function of the excitation pulse width for CS2 with and without Cy3. Figura 2H.dat - FRET efficiency calculated from data of figure (G). Figura 3A.dat - UCL luminescence lifetime as a function of laser power for CS1 with and without dye for 750 μs pulse width. Figura 3B.dat - FRET efficiency from data shown in Figure 3A Figura 3C.dat - UCL lifetime from Figure 2C and Figure 3A as a function of laser pulse energy. Figura 3D.dat - FRET efficiency from the UCL lifetime data shown in Figure 3C. Figura 4B.dat - Simulated green UCL lifetime as a function of the excitation pulse width for both with and without Cy3. Figura 4C.dat - Simulated and experimental FRET efficiency calculated from the results shown in Figure 4B. Figura 4D.dat - Simulated green UCL lifetime as a function of the excitation pulse width for both with and without Cy3. Figura 4E.dat - Simulated and experimental FRET efficiency calculated from the results shown in Figure 4D. -------------------------- DERECHOS DE USO Y PRIVACIDAD -------------------------- 1. Licencias de uso Creative Commons Attribution 4.0 International 2. Privacidad -------------------------- OTROS --------------------------