Person:
García García, Isabel

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First Name
Isabel
Last Name
García García
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Department
Genética, Fisiología y Microbiología
Area
Identifiers
UCM identifierORCIDScopus Author IDDialnet ID

Search Results

Now showing 1 - 5 of 5
  • Publication
    Laboratorio en abierto: aprendendiendo a copiar ADN.2
    (2022-09-30) Linacero de la Fuente, Rosario; Espino Nuño, Francisco Javier; Gallego Rodríguez, Francisco Javier; Pradillo Orellana, Mónica; Callejas Hervás, Carmen; González Sánchez, Mónica; Arana Montes, María del Pilar De; Ballesteros, Isabel; Cuñado Rodríguez, Nieves; González Jaén, María Teresa; Figueiras Merino, Ana Margarita; Gil Dones, Félix; Peña Gómez, Alicia de la; Hórreo Escandón, José Luis; Gorfinkiel, Nicole; Vega Melero, Juan Manuel; Barrios Diaz, Jose; Llanos Saavedra, Carolina; Moreno Ortiz, María del Carmen; Fernández Jiménez, Nadia; Méndez Cea, Belén; García García, Isabel; Muñoz Muñoz, María
    El objetivo principal del proyecto es la puesta a punto de recursos educativos en abierto (REA) dirigidos a los alumnos de secundaria. La propuesta pretende desarrollar habilidades, para la resolución de problemas científicos, a través de retos que despierten el interés y la imaginación de los alumnos de secundaria. En esta propuesta la resolución de los problemas planteados estaría basada en la aplicación de una herramienta que ha revolucionado la genética y biología, la reacción en Cadena de la Polimerasa, conocida como PCR.
  • Publication
    Challenges and Perspectives in the Epigenetics of Climate Change-Induced Forests Decline
    (Frontiers Media, 2022-01-04) García García, Isabel; Méndez Cea, Belén; Martín Gálvez, David; Seco, José Ignacio; Gallego Rodríguez, Francisco Javier; Linares, Juan Carlos
    Forest tree species are highly vulnerable to the effects of climate change. As sessile organisms with long generation times, their adaptation to a local changing environment may rely on epigenetic modifications when allele frequencies are not able to shift fast enough. However, the current lack of knowledge on this field is remarkable, due to many challenges that researchers face when studying this issue. Huge genome sizes, absence of reference genomes and annotation, and having to analyze huge amounts of data are among these difficulties, which limit the current ability to understand how climate change drives tree species epigenetic modifications. In spite of this challenging framework, some insights on the relationships among climate change-induced stress and epigenomics are coming. Advances in DNA sequencing technologies and an increasing number of studies dealing with this topic must boost our knowledge on tree adaptive capacity to changing environmental conditions. Here, we discuss challenges and perspectives in the epigenetics of climate change-induced forests decline, aiming to provide a general overview of the state of the art.
  • Publication
    Laboratorio en abierto: aPrendiendo a CopiaR el ADN
    (2021-09-30) Linacero de la Fuente, Rosario; Espino Nuño, Francisco Javier; Pradillo Orellana, Mónica; Gallego Rodríguez, Francisco Javier; González Sánchez, Mónica; Arana Montes, María del Pilar; Beroiz Remirez, Beatriz; Cuñado Rodríguez, Nieves; Figueiras Merino, Ana Margarita; Gil Dones, Félix; de la Peña Gómez, Alicia; Vega Melero, Juan Manuel; Barrios Díaz, José; Llanos Saavedra, Carolina; Moreno Ortiz, María del Carmen; Blasio, Francesco; Fernández Jiménez, Nadia; García García, Isabel; Lantero Bringas, Esther; Lira Garrido, Jaime; Martínez García, Marina; Méndez Cea, Belén; Matallanas Peñas, Beatriz
    El objetivo principal del proyecto es la puesta a punto de recursos educativos en abierto (REA) dirigidos a los alumnos de secundaria. El punto de partida será plantear diferentes retos y situaciones que se pueden resolver utilizando distintos recursos científicos, para decidir qué recurso es el más adecuado y cómo se aplica. En esta propuesta la resolución de los problemas planteados estaría basada en la aplicación de una herramienta que ha revolucionado la genética y biología, la reacción en Cadena de la Polimerasa, conocida como PCR.
  • Publication
    Tree-level growth patterns and genetic associations depict drought legacies in the relict forests of Abies Marocana
    (MDPI, 2023-02-15) Méndez Cea, Belén; García García, Isabel; Sánchez Salguero, Raúl; Lechuga, Víctor; Gallego Rodríguez, Francisco Javier; Linares, Juan C.
    The frequency and intensity of drought events are increasing worldwide, challenging the adaptive capacity of several tree species. Here, we evaluate tree growth patterns and climate sensitivity to precipitation, temperature, and drought in the relict Moroccan fir Abies marocana. We selected two study sites, formerly stated as harboring contrasting A. marocana taxa (A. marocana and A. tazaotana, respectively). For each tree, dendrochronological methods were applied to quantify growth patterns and climate–growth sensitivity. Further, ddRAD-seq was performed on the same trees and close saplings to obtain single nucleotide polymorphisms (SNPs) and related genotype–phenotype associations. Genetic differentiation between the two studied remnant populations of A. marocana was weak. Growth patterns and climate–growth relationships were almost similar at the two sites studied, supporting a negative effect of warming. Growth trends and tree size showed associations with SNPs, although there were no relationships with phenotypes related to climatic sensitivity. We found significant differences in the SNPs subjected to selection in the saplings compared to the old trees, suggesting that relict tree populations might be subjected to genetic differentiation and local adaptation to climate dryness. Our results illustrate the potential of tree rings and genome-wide analysis to improve our understanding of the adaptive capacity of drought-sensitive forests to cope with ongoing climate change.
  • Publication
    DNA extraction and amplification from Pinaceae dry wood
    (Sciendo, 2019) Méndez-Cea, Belén; Cobo Simón, Irene; Pérez González, Ana; García García, Isabel; Linares, Juan Carlos; Gallego Rodríguez, Francisco Javier
    Wood constitutes the unique source of DNA in dead trees, but extraction of adequate quality DNA from dry wood is usually challenging. However, many different molecular studies require the use of such DNA. We have standardized and validated a modified CTAB protocol to isolate DNA from dry wood from Abies pinsapo and Cedrus atlantica species. Due to the degradation and very little DNA that is normally present in the wood from dead trees we have developed a PCR based test to certify the quality of the extracted samples. In the present study, we have proved too the effectiveness of this methodology to isolate DNA from conifer dry wood samples of sufficient quality to perform further molecular genetic experiments.