Person: Pastor Vargas, Carlos
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First Name
Carlos
Last Name
Pastor Vargas
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Bioquímica y Biología Molecular
Area
Bioquímica y Biología Molecular
Identifiers
50 results
Search Results
Now showing 1 - 10 of 50
Item Targeting antigens to an invariant epitope of the MHC Class II DR molecule potentiates the immune response to subunit vaccines(Virus Research, 2011) Gil Dones, Félix; Pérez-Filgueira, Mariano; Barderas, María G.; Pastor Vargas, Carlos; Alonso, Covadonga; Vivanco, Fernando; Escribano, José M.Recombinant subunit and peptidic vaccines in general present a reduced immunogenicity in vaccinated individuals with respect to the whole pathogen from which they derived. The generation of strong immune responses to these vaccines requires the use of potent adjuvants, high antigen doses and repetitive vaccinations. In this report, we document the enhanced antibody response obtained against two recombinant subunit vaccines by means of targeting to antigen-presenting cells by a recombinant single chain antibody. This antibody, named APCH1, recognizes an epitope of MHC Class II DR molecule preserved in different animal species, including humans. We showed that vaccinal antigens translationally fused to APCH1 antibody and produced by recombinant baculoviruses in insect larvae (Trichoplusia ni), elicited an increased antibody response in comparison with the same antigens alone or fused to a carrier molecule. These results suggest that targeting of antigens to this invariant MHC Class II epitope has immunopotentiating effects that could circumvent the reduced potency of peptidic or subunit vaccines, opening the possibility of widespread application of APCH1 as a new adjuvant antibody of general use.Item Targeting antigens to an invariant epitope of the MHC Class II DR molecule potentiates the immune response to subunit vaccines(Virus Research, 2011) Pérez-Filgueira, Mariano; Barderas, María G.; Alonso, Covadonga; José M, Escribano; Gil Dones, Félix; Pastor Vargas, Carlos; Vivanco Martínez, FernandoRecombinant subunit and peptidic vaccines in general present a reduced immunogenicity in vaccinated individuals with respect to the whole pathogen from which they derived. The generation of strong immune responses to these vaccines requires the use of potent adjuvants, high antigen doses and repetitive vaccinations. In this report, we document the enhanced antibody response obtained against two recombinant subunit vaccines by means of targeting to antigen-presenting cells by a recombinant single chain antibody. This antibody, named APCH1, recognizes an epitope of MHC Class II DR molecule preserved in different animal species, including humans. We showed that vaccinal antigens translationally fused to APCH1 antibody and produced by recombinant baculoviruses in insect larvae (Trichoplusia ni), elicited an increased antibody response in comparison with the same antigens alone or fused to a carrier molecule. These results suggest that targeting of antigens to this invariant MHC Class II epitope has immunopotentiating effects that could circumvent the reduced potency of peptidic or subunit vaccines, opening the possibility of widespread application of APCH1 as a new adjuvant antibody of general use.Item Occupational asthma caused by gerbil: purification and partial characterization of a new gerbil allergen(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2010) de las Heras, Manuel; Cuesta-Herranz, Javier; Cases, Bárbara; de Miguel, Jaime; Fernández-Nieto, Mar; Sastre, Joaquin; Vivanco Martínez, Fernando; Pastor Vargas, CarlosItem Allergy to Pumpkin With Cyclophilin as the Relevant Allergen(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2010) González de Olano, David; González-Mancebo, Eloina; Santos Macadán, Sara; Gandolfo Cano, Mar; Pérez-Gordo, Marina; Cases Ortega, Bárbara; Vivanco Martínez, Fernando; Pastor Vargas, CarlosItem Identification of potential allergens involved in systemic reactions to melon and watermelon(Annals of allergy, asthma & immunology, 2010) González-Mancebo, Eloina; López-Torrejón, Gema; González de Olano, David; Cembellín Santos, Sara; Gandolfo-Cano, Mar; Meléndez, Amaya; Salcedo, Gabriel; Cuesta-Herranz, Javier; Vivanco Martínez, Fernando; Pastor Vargas, CarlosItem New allergen involved in a case of allergy to Solea solea, common sole(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2010) Pérez-Gordo, Marina; Pastor Vargas, Carlos; Cases, Bárbara; De Las Heras, Manuel; Sanz, Aroa; Vivanco Martínez, Fernando; Cuesta-Herranz, JavierItem Allergy to Prairie Dog Lipocalins(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2010) González de Olano, David; Bartolomé, Borja; Cases Ortega, Bárbara; Pérez-Gordo, Marina; Vivanco Martínez, Fernando; Pastor Vargas, CarlosItem Identification of allergens in chicken meat allergy.(J Investig Allergol Clin Immunol, 2011) Eloina González-Mancebo, Eloina; Pastor Vargas, Carlos; González-de-Olano, David; Gandolfo-Cano, Mar; Melendez, Amaya; Cuesta Herranz, Javier; Zapatero, A.; Bartolome, BorjaItem Cross‐reactivity between coconut and lentil related to a 7S globulin and an 11S globulin(Allergy, 2010) Manso, L.; Pastor, C.; Pérez‐Gordo, M.; Cases, B.; Sastre, J.; Cuesta‐Herranz, J.; Pastor Vargas, CarlosItem Phosphorylation reduces the allergenicity of cow casein in children with selective allergy to goat and sheep milk.(J Investig Allergol Clin Immunol, 2011) Cases, Barbara; García-Ara, Carmen; Boyano, Maria Teresa; Pérez-Gordo, Marina; Pedrosa, Maria; Vivanco Martínez, Fernando; Quirce, Santiago; Pastor Vargas, CarlosThis study aimed to characterize the role of phosphorylation of caseins in selective allergy to goat milk (GM) and sheep milk (SM) in patients with good tolerance to cow milk (CM). We performed skin prick tests with milk and caseins from CM, GM, and SM and immunoblotting and specific immunoglobulin (Ig) E determinations with milk and casein from cow and GM and SM. Sensitization to milk and caseins from goat and sheep was demonstrated in all 3 patients by skin tests, determination of specific IgE, or both. Immunoblotting confirmed that GM/SM proteins but not CM proteins were involved in the allergic symptoms. IgE reacted with several protein bands from the caseins and milk extracts of both sheep and goat. Phosphorylation was involved in the different allergenicity of CM caseins. We report the implication of phosphorylation in the allergenicity of caseins involved in selective allergy to GM and SM.