Person: Rancán, Lisa
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First Name
Lisa
Last Name
Rancán
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Bioquímica y Biología Molecular
Area
Bioquímica y Biología Molecular
Identifiers
12 results
Search Results
Now showing 1 - 10 of 12
- Project number: 252
Item Protagonizando la generación de conocimiento a través de la integración de la investigación básica y clínica: Del quirófano al laboratorio(2017) Paredes Royano, Sergio Damián; Rancán, Lisa; García Martín, M. Cruz; Garutti Martínez, Ignacio; Simón Adiego, Carlos María; Zueco Alegre, José Antonio; Fernández-Tresguerres Hernández, Jesús Ángel; Vara Ameigeiras, Elena María Item Sevoflurane prevents liver inflammatory response induced by lung ischemia-reperfusion.(Transplantation., 2014) Rancán, Lisa; Huerta Martínez, Luis Javier; Cusati, G; Erquicia, I; Isea, J; Paredes Royano, Sergio Damián; García, C; Garutti Martínez, Ignacio; Simón Adiego, Carlos María; Vara Ameigeiras, Elena MaríaBackground: Transplants cause ischemia-reperfusion (IR) injury that can affect distant organs. Liver is particularly sensitive to IR injury. The present randomized experimental study was designed to investigate a possible protective effect of sevoflurane against liver inflammatory response to lung IR in a lung upper lobe left autotransplant model. Methods: Two groups (sevoflurane and control) of eight swines each were submitted to upper lobe left lung autotransplant. Hypnotic maintenance was performed with sevoflurane 3% or propofol 8 to 10 mg/kg per hr until pneumonectomy was done; then propofol was used for all animals. Blood and liver samples were taken in four different moments: prepneumonectomy, prereperfusion, 10 min postreperfusion and 30 min postreperfusion to measure levels of interleukin (IL)-1β, IL-10, tumor necrosis factor (TNF)-α, monocyte chemotactic protein (MCP)-1, nuclear factor (NF)-κB, C-reactive protein, ferritin and caspase 3. Non-parametric test was used to find statistical meaning. Results: Lung IR markedly increased the expression of TNF-α, IL-1β, MCP-1, NF-κB and caspase activity in control livers compared with basal levels, whereas liver IL-10 expression decreased 10 and 30 min post-reperfusion. Sevoflurane significantly decreased TNF-α, IL-1β, MCP-1, NF-κB liver expression and caspase 3 activity. Sevoflurane also reverted the lung IR-induced decrease in IL-10 expression. Conclusions: The present results indicate that lung IR caused hepatic injury. Sevoflurane attenuated liver injury in a model of upper lobe left lung autotransplant in pigs.Item Ischaemic preconditioning prevents the liver inflammatory response to lung ischaemia/reperfusion in a swine lung autotransplant model†(European Journal of Cardio-Thoracic Surgery, 2012) Huerta Martínez, Luis Javier; Rancán, Lisa; Simón Adiego, Carlos María; Vidaurre, Eduardo; Isea, Jesús; Vara Ameigeiras, Elena María; Garutti Martínez, Ignacio; González Aragoneses, FedericoOBJECTIVES: Lung ischaemia/reperfusion (IR) induces a systemic inflammatory response that causes damage to remote organs. The liver is particularly sensitive to circulating inflammatory mediators that occur after IR of remote organs. Recently, remote ischaemic preconditioning has been proposed as a surgical tool to protect several organs from IR. The present study was designed to investigate a possible protective effect of lung ischaemic preconditioning (IP) against the liver inflammatory response to lung IR. METHODS: Two groups [IP and control (CON)] of 10 Large White pigs underwent lung autotransplants (left pneumonectomy, ex situ cranial lobectomy and caudal lobe reimplantation). Before pneumonectomy was performed in the study group, IP was induced with two 5-min cycles of left pulmonary arterial occlusion and a 5-min interval of reperfusion between the two occlusions. Five animals underwent sham surgery. Liver biopsies were obtained during surgery at (i) prepneumonectomy, (ii) prereperfusion, (iii) 10 min after reperfusion of the implanted lobe and (iv) 30 min after reperfusion. The expression of tumor necrosis factor-α (TNF-α), interleukin (IL)-1, IL-10 and inducible form of nitric oxide synthase (iNOS) was analysed by western blotting. The expression of mRNA for TNF-α, IL1, IL-10, monocyte chemoattractant protein-1 (MCP-1), nuclear factor kappa beta and iNOS was analysed by reverse transcription–polymerase chain reaction. Caspase-3 activity was determined by enzyme-linked immunosorbent assay. Non-parametric tests were used to compare differences between and within groups. RESULTS: Lung IR markedly increased expression of TNF-α (P = 0.0051) and IL-1 (P = 0.0051) and caspase-3 activity (P = 0.0043) in the CON group compared with the prepneumonectomy levels. A decrease of IL-10 mRNA expression was observed in the CON group after lung reperfusion. In the IP group, TNF-α (P = 0.0011) and IL-1 (P = 0.0001) expression and caspase-3 activity (P < 0.0009) were lower after reperfusion than in the CON group. IP caused reversion of the observed decrease of IL-10 mRNA expression (P = 0.016) induced in liver tissue by lung IR. Lung IR markedly increased the expression of mRNA MCP-1 after 10 min (P = 0.0051) and 30 min (P = 0.0051) of reperfusion. These increases were not observed in the IP or sham groups. CONCLUSIONS: IP prevented liver injury induced by lung IR through the reduction of proinflammatory cytokines and hepatocyte apoptosis.- Project number: 223
Item Uso del debate formal como herramienta de innovación docente para el desarrollo temprano de las habilidades de comunicación y el análisis crítico de los estudiantes de Ciencias de la Salud(2018) Paredes Royano, Sergio Damián; Rancán, Lisa; Alonso González, Alberto; García Martín, M. Cruz; García Pérez, Juan Carlos; Garutti Martínez, Ignacio; Simón Adiego, Carlos María; Zueco Alegre, José Antonio; Fernández-Tresguerres Hernández, Jesús Ángel; Vara Ameigeiras, Elena María; Alonso González, Luis Alberto Item Intravenous Lidocaine Decreases Tumor Necrosis Factor Alpha Expression Both Locally and Systemically in Pigs Undergoing Lung Resection Surgery(Anesthesia & Analgesia, 2014) Garutti Martínez, Ignacio; Rancán, Lisa; Simón Adiego, Carlos María; López Gil, María Teresa; Cusati, Gabriel; Sanchez-Pedrosa, Guillermo; Moraga, Francisco; Olmedilla, Luis; Vara Ameigeiras, Elena MaríaBackground: Lung resection surgery is associated with an inflammatory reaction. The use of 1-lung ventilation (OLV) seems to increase the likelihood of this reaction. Different prophylactic and therapeutic measures have been investigated to prevent lung injury secondary to OLV. Lidocaine, a commonly used local anesthetic drug, has antiinflammatory activity. Our main goal in this study was to investigate the effect of IV lidocaine on tumor necrosis factor α (TNF-α) lung expression during lung resection surgery with OLV. Methods: Eighteen pigs underwent left caudal lobectomy. The animals were divided into 3 groups: control, lidocaine, and sham. All animals received general anesthesia. In addition, animals in the lidocaine group received a continuous IV infusion of lidocaine during surgery (1.5 mg/kg/h). Animals in the sham group only underwent thoracotomy. Samples of bronchoalveolar lavage (BAL) fluid and plasma were collected before initiation of OLV, at the end of OLV, at the end of surgery, and 24 hours after surgery. Lung biopsy specimens were collected from the left caudal lobe (baseline) before surgery and from the mediastinal lobe and the left cranial lobe 24 hours after surgery. Samples were flash-frozen and stored to measure levels of the following inflammatory markers: interleukin (IL) 1β, IL-2, IL-10, TNF-α, nuclear factor κB, monocyte chemoattractant protein-1, inducible nitric oxide synthase, and endothelial nitric oxide synthase. Markers of apoptosis (caspase 3, caspase 9, Bad, Bax, and Bcl-2) were also measured. In addition, levels of metalloproteinases and nitric oxide metabolites were determined in BAL fluid and in plasma samples. A nonparametric test was used to examine statistical significance. Results: OLV caused lung damage with increased TNF-α expression in BAL, plasma, and lung samples. Other inflammatory (IL-1β, nuclear factor κB, monocyte chemoattractant protein-1) and apoptosis (caspase 3, caspase 9, and BAX) markers were also increased. With the use of IV lidocaine there was a significant decrease in the levels of TNF-α in the same samples compared with the control group. Lidocaine administration also reduced the inflammatory and apoptotic changes observed in the control group. Hemodynamic values, blood gas values, and airway pressure were similar in all groups. Conclusions: Our results suggest that lidocaine can prevent OLV-induced lung injury through reduced expression of proinflammatory cytokines and lung apoptosis. Administration of lidocaine may help to prevent lung injury during lung surgery with OLV.Item Lidocaine Administration Controls MicroRNAs Alterations Observed After Lung Ischemia–Reperfusion Injury(Anesthesia & Analgesia, 2016) Rancán, Lisa; Simón Adiego, Carlos María; Marchal-Duval, Emmeline; Casanova, Javier; Paredes Royano, Sergio Damián; Calvo, Alberto; García Martín, M. Cruz; Rincón, David; Turrero Nogués, Agustín; Garutti Martínez, Ignacio; Vara Ameigeiras, Elena MaríaBACKGROUND: Ischemia–reperfusion injury (IRI) is associated with morbidity and mortality. MicroRNAs (miRNAs) have emerged as regulators of IRI, and they are involved in the pathogenesis of organ rejection. Lidocaine has proven anti-inflammatory activity in several tissues but its modulation of miRNAs has not been investigated. This work aims to investigate the involvement of miRNAs in lung IRI in a lung auto-transplantation model and to investigate the effect of lidocaine. METHODS: Three groups (sham, control, and Lidocaine), each comprising 6 pigs, underwent a lung autotransplantation. All groups received the same anesthesia. In addition, animals of lidocaine group received a continuous intravenous administration of lidocaine (1.5 mg/kg/h) during surgery. Lung biopsies were taken before pulmonary artery clamp, before reperfusion, 30 minutes postreperfusion (Rp-30), and 60 minutes postreperfusion (Rp-60). Samples were analyzed for different miRNAs (miR-122, miR-145, miR-146a, miR-182, miR-107, miR-192, miR-16, miR-21, miR-126, miR-127, miR142-5p, miR152, miR155, miR-223, and let7) via the use of reverse-transcription quantitative polymerase chain reaction. Results were normalized with miR-103. RESULTS: The expression of miR-127 and miR-16 did not increase after IRI. Let-7d, miR-21, miR-107, miR-126, miR-145, miR-146a, miR-182, and miR-192 significantly increased at the Rp-60 (control versus sham P < .001). miR-142-5p, miR-152, miR-155, and miR 223 significantly increased at the Rp-30 (control versus sham P < .001) and at the Rp-60 (control versus. sham P < .001). The administration of lidocaine was able to attenuate these alterations in a significant way (control versus Lidocaine P < .001). CONCLUSIONS: Lung IRI caused dysregulation miRNA. The administration of lidocaine reduced significantly miRNAs alterations.- Project number: 154
Item Mejora de las habilidades comunicativas y el pensamiento crítico en estudiantes de Ciencias de la Salud mediante la combinación de flipped classroom (clase invertida) y debate formal(2019) Paredes Royano, Sergio Damián; Rancán, Lisa; Alonso González, Luis Alberto; Asencio Pascual, José Manuel; García Martín, M. Cruz; Garutti Martínez, Ignacio; Huerta Martínez, Luis Javier; Marañón Pardillo, Gonzalo; Simón Adiego, Carlos María; Valdés López-Linares, Sergio; Valdivielso Suárez, Elena; Zueco Alegre, José Antonio; Vara Ameigeiras, Elena MaríaSe propone la clase invertida (flipped classroom) y el debate formal como metodologías innovadoras para mejorar las habilidades comunicativas y el pensamiento crítico en respuesta a la demanda de tener futuros profesionales biomédicos mejor formados en estos ámbitos. Item Effects of Intraoperative Infusion of Esmolol on Systemic and Pulmonary Inflammation in a Porcine Experimental Model of Lung Resection Surgery(Anesthesia & Analgesia, 2019) Garutti Martínez, Ignacio; Rancán, Lisa; Abubakra, Selma; Simón Adiego, Carlos María; Paredes Royano, Sergio Damián; Ortega, Javier; Huerta Martínez, Luis Javier; Nieva Ramos, Silvia; Vara Ameigeiras, Elena MaríaAbstract BACKGROUND: Lung resection surgery (LRS) is associated with systemic and pulmonary inflammation, which can affect postoperative outcomes. Activation of β-adrenergic receptors increases the expression of proinflammatory and anti-inflammatory mediators, and their blockade may attenuate the systemic inflammatory response. The aim of this study was to analyze the effect of a continuous perioperative intravenous perfusion of esmolol on postoperative pulmonary edema in an experimental model of LRS requiring periods of one-lung ventilation (OLV). METHODS: Twenty-four large white pigs were randomly assigned to 3 groups: control (CON), esmolol (ESM), and sham. The ESM group received an intravenous esmolol bolus (0.5 mg/kg) and then an esmolol infusion (0.05 mg·kg−1·minute−1) throughout the procedure. The CON group received the same volume of 0.9% saline solution as the ESM group plus a continual infusion of saline. The sham group underwent a left thoracotomy without LRS or OLV. At the end of the LRS, the animals were awakened, and after 24 hours, they underwent general anesthesia again. Lung biopsies and plasma samples were obtained to analyze the levels and expression of inflammatory mediators, and the animals also received a bronchoalveolar lavage. RESULTS: At 24 hours after the operation, the ESM group had less lung edema and lower expression of the proinflammatory biomarkers tumor necrosis factor (TNF) and interleukin (IL)-1 compared to the CON group for both lung lobes. For the mediastinal lobe biopsies, the mean difference and 95% confidence interval (CI) between the groups for edema, TNF, and IL-1 were 14.3 (95% CI, 5.6–23.1), P = .002; 0.19 (95% CI, 0.07–0.32), P = .002; and 0.13 (95% CI, 0.04–0.22), P = .006, respectively. In the left upper lobe, the mean differences for edema, TNF, and IL-1 were 12.4 (95% CI, 4.2–20.6), P = .003; 0.25 (95% CI, 0.12–0.37), P < .001; and 0.3 (95% CI, 0.08–0.53), P = .009. CONCLUSIONS: Our results suggest that esmolol reduces lung edema and inflammatory responses in the intraoperative and postoperative periods in animals that underwent LRS with OLV.- Project number: 234
Item Clima Educativo en Medicina: Oportunidades de Mejora(2022) Vara Ameigeiras, Elena María; Arias Díaz, Javier; Alonso González, Luis Alberto; Bourquia, Marwane; Corrales Fernández, Carmen; Garcia Marín, María Cruz; Garutti Martínez, Ignacio; Giner Nogueras, Manuel; Gutiérrez Vierna, María Elvira; Idrissi, Hassana; Linillos Pradillo, Beatriz; Millán Núñez-Cortés, Jesús; Paredes Royano, Sergio Damián; Rancán, Lisa; Simón Adiego, Carlos María; Valdés López-Linares, Sergio; Zueco Alegre, José AntonioEl entorno donde se desarrolla la formación, las relaciones alumno-profesor, los aspectos físicos (facultad/hospital) como hábitat, etc. es percibido por los alumnos influyendo en su comportamiento y lo determinan de forma importante, condicionando la consecución de los objetivos, la satisfacción y el éxito. Si el entorno educativo no es adecuado, se dificulta la adquisición de conocimientos y/o de valores de los futuros profesionales y las facultades de medicina deben reflexionar seriamente para aplicar los cambios necesarios para mejorar la enseñanza. En la enseñanza, tan importante es establecer un contexto o clima para el aprendizaje adecuado, como impartir conocimientos o compartir experiencias. Se propone identificar las deficiencias, es decir, los aspectos críticos del clima de aprendizaje como base para las medidas de optimización, así como las fortalezas de un currículo o plan de estudios. Observar las expectativas de los estudiantes con las actuales experiencias, para identificar puntos que requieran mejoras y comparar el ambiente de aprendizaje en diferentes lugares y etapas, entre los estudiantes de los diferentes cursos del Grado en Medicina de la Universidad Complutense de Madrid. Item Effects of Intraoperative Infusion of Esmolol on Systemic and Pulmonary Inflammation in a Porcine Experimental Model of Lung Resection Surgery.(Anesthesia and analgesia, 2019) Garutti Martínez, Ignacio; Rancán, Lisa; Abubakra, S; Simón Adiego, Carlos María; Paredes Royano, Sergio Damián; Ortega, J; Huerta Martínez, Luis Javier; Ramos, S; Vara Ameigeiras, Elena MaríaBackground: Lung resection surgery (LRS) is associated with systemic and pulmonary inflammation, which can affect postoperative outcomes. Activation of β-adrenergic receptors increases the expression of proinflammatory and anti-inflammatory mediators, and their blockade may attenuate the systemic inflammatory response. The aim of this study was to analyze the effect of a continuous perioperative intravenous perfusion of esmolol on postoperative pulmonary edema in an experimental model of LRS requiring periods of one-lung ventilation (OLV). Methods: Twenty-four large white pigs were randomly assigned to 3 groups: control (CON), esmolol (ESM), and sham. The ESM group received an intravenous esmolol bolus (0.5 mg/kg) and then an esmolol infusion (0.05 mg·kg·minute) throughout the procedure. The CON group received the same volume of 0.9% saline solution as the ESM group plus a continual infusion of saline. The sham group underwent a left thoracotomy without LRS or OLV. At the end of the LRS, the animals were awakened, and after 24 hours, they underwent general anesthesia again. Lung biopsies and plasma samples were obtained to analyze the levels and expression of inflammatory mediators, and the animals also received a bronchoalveolar lavage. Results: At 24 hours after the operation, the ESM group had less lung edema and lower expression of the proinflammatory biomarkers tumor necrosis factor (TNF) and interleukin (IL)-1 compared to the CON group for both lung lobes. For the mediastinal lobe biopsies, the mean difference and 95% confidence interval (CI) between the groups for edema, TNF, and IL-1 were 14.3 (95% CI, 5.6-23.1), P = .002; 0.19 (95% CI, 0.07-0.32), P = .002; and 0.13 (95% CI, 0.04-0.22), P = .006, respectively. In the left upper lobe, the mean differences for edema, TNF, and IL-1 were 12.4 (95% CI, 4.2-20.6), P = .003; 0.25 (95% CI, 0.12-0.37), P < .001; and 0.3 (95% CI, 0.08-0.53), P = .009. Conclusions: Our results suggest that esmolol reduces lung edema and inflammatory responses in the intraoperative and postoperative periods in animals that underwent LRS with OLV.