Person:
Parrado Quintela, Concepción

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First Name
Concepción
Last Name
Parrado Quintela
URI
https://hdl.handle.net/20.500.14352/81547
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
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Author: Diaz, Raúl × Start date: 2021 ×

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    Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor
    (Biosensors, 2022) Villalonga Chico, Anabel; Parrado Quintela, Concepción; Diaz, Raúl; Sánchez Sánchez, Alfredo; Mayol Hornero, Beatriz; Martínez-Ruíz, Paloma; Vilela García, Diana; Villalonga Santana, Reynaldo
    Here we report a novel labeling strategy for electrochemical aptasensors based on enzymatic marking via supramolecular host–guest interactions. This approach relies on the use of an adamantane-modified target-responsive hairpin DNA aptamer as an affinity bioreceptor, and a neoglycoconjugate of β-cyclodextin (CD) covalently attached to a redox enzyme as a labeling element. As a proof of concept, an amperometric aptasensor for a carcinoembryonic antigen was assembled on screen-printed carbon electrodes modified with electrodeposited fern-like gold nanoparticles/graphene oxide and, by using a horseradish peroxidase-CD neoglycoenzyme as a biocatalytic redox label. This aptasensor was able to detect the biomarker in the concentration range from 10 pg/mL to 1 ng/mL with a high selectivity and a low detection limit of 3.1 pg/mL in human serum samples.