Person:
Campuzano Ruiz, Susana

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First Name
Susana
Last Name
Campuzano Ruiz
URI
https://hdl.handle.net/20.500.14352/76074
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
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Author: Blázquez García, Marina × Has files: true ×

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    Binary MoS2 nanostructures as nanocarriers for amplification in multiplexed electrochemical immunosensing: simultaneous determination of B cell activation factor and proliferation-induced signal immunity-related cytokines
    (Microchimica Acta, 2022) Arévalo Pérez, Beatriz; Blázquez García, Marina; Valverde de la Fuente, Alejandro; Serafín González-Carrato, Verónica; Montero Calle, Ana; Solís Fernández, Guillermo; Barderas Manchado, Rodrigo; Campuzano Ruiz, Susana; Yáñez-Sedeño Orive, Paloma; Pingarron, José M.
    A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specifc antibodies (cAbs) and a strategy for signal amplifcation based on labelling the detection antibodies (dAbs) with binary MoS2/ MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng mL−1 for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.
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    Project number: PIMCD320/23-24
    Contribuyendo a la internacionalización de la docencia práctica inclusiva y sostenible en Química Analítica
    (0024) Gamella Carballo, María; Pedrero Muñoz, María; Campuzano Ruiz, Susana; Serafín González-Carrato, Verónica; Torrente Rodríguez, Rebeca Magnolia; Ruiz Valdepeñas Montiel, Víctor; Valverde De La Fuente, Alejandro; Povedano Muñumel, Eloy; Muñoz San Martín, Cristina; Pérez Ginés, Víctor; Blázquez García, Marina; Tejerina Miranda, Sandra; de Valle Ávila, Marcos; Molla Escudero, David