Person:
Campuzano Ruiz, Susana

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First Name
Susana
Last Name
Campuzano Ruiz
URI
https://hdl.handle.net/20.500.14352/76074
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
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2000 - 20242
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Author: Blázquez García, Marina ×

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Now showing 1 - 3 of 3
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    Angiogenesis inhibitor or aggressiveness marker? The function of endostatin in cancer through electrochemical biosensing
    (Bioelectrochemistry, 2024) Tejerina Miranda, Sandra; Pedrero Muñoz, María; Blázquez García, Marina; Serafín González-Carrato, Verónica; Montero Calle, Ana; Garranzo Asensio, María; Reviejo García, Ángel Julio; Pingarrón Carrazón, José Manuel; Barderas Manchado, Rodrigo; Campuzano Ruiz, Susana
    This work reports the first electrochemical bioplatform developed for the determination of human endostatin (HE), a biomarker with recognized antiangiogenic potential whose elevated circulating levels have also been associated with the development of aggressive cancers. The developed electroanalytical biotool combines the benefits of using magnetic microparticles for the implementation of sandwich immunoassays and amperometric transduction on disposable carbon electrodes. A limit of detection (LOD) of 34.1 pg mL−1 for HE standards and a selectivity suitable for its foray into the clinical oncology area, are demonstrated. The determination of HE in clinical samples such as lysates and secretomes of colorectal cancer (CRC) cells, plasma, and tissue samples from patients with CRC in different stages, has been faced with satisfactory results showing the ability for discriminating the metastatic capabilities of cells and for identifying and staging CRC patients. The developed bioplatform allows precise quantitative determinations, requiring minimal pre-treatments and sample amounts in only 75 min. In addition, due to the instrumentation and the type of substrates used in the detection step, the biotool is compatible with implementation in multiplexed and/or point-of-need devices, features in which this bioplatform is advantageous with respect to the enzyme linked immunosorbent assay (ELISA) or immunoblotting technologies.
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    Binary MoS2 nanostructures as nanocarriers for amplification in multiplexed electrochemical immunosensing: simultaneous determination of B cell activation factor and proliferation-induced signal immunity-related cytokines
    (Microchimica Acta, 2022) Arévalo Pérez, Beatriz; Blázquez García, Marina; Valverde de la Fuente, Alejandro; Serafín González-Carrato, Verónica; Montero Calle, Ana; Solís Fernández, Guillermo; Barderas Manchado, Rodrigo; Campuzano Ruiz, Susana; Yáñez-Sedeño Orive, Paloma; Pingarron, José M.
    A dual immunosensor is reported for the simultaneous determination of two important immunity-related cytokines: BAFF (B cell activation factor) and APRIL (a proliferation-induced signal). Sandwich-type immunoassays with specifc antibodies (cAbs) and a strategy for signal amplifcation based on labelling the detection antibodies (dAbs) with binary MoS2/ MWCNTs nanostructures and using horseradish peroxidase (HRP) were implemented. Amperometric detection was carried out at screen-printed dual carbon electrodes (SPdCEs) through the hydroquinone HQ/H2O2 system. The developed dual immunosensor provided limit of detection (LOD) of 0.08 and 0.06 ng mL−1 for BAFF and APRIL, respectively, and proved to be useful for the determination of both cytokines in cancer cell lysates and serum samples from patients diagnosed with autoimmune diseases and cancer. The obtained results agreed with those found using ELISA methodologies.
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    Project number: PIMCD320/23-24
    Contribuyendo a la internacionalización de la docencia práctica inclusiva y sostenible en Química Analítica
    (0024) Gamella Carballo, María; Pedrero Muñoz, María; Campuzano Ruiz, Susana; Serafín González-Carrato, Verónica; Torrente Rodríguez, Rebeca Magnolia; Ruiz Valdepeñas Montiel, Víctor; Valverde De La Fuente, Alejandro; Povedano Muñumel, Eloy; Muñoz San Martín, Cristina; Pérez Ginés, Víctor; Blázquez García, Marina; Tejerina Miranda, Sandra; de Valle Ávila, Marcos; Molla Escudero, David