Person: Vivanco Martínez, Fernando
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First Name
Fernando
Last Name
Vivanco Martínez
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Area
Bioquímica y Biología Molecular
Identifiers
6 results
Search Results
Now showing 1 - 6 of 6
Item Allergy to Pumpkin With Cyclophilin as the Relevant Allergen(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2010) González de Olano, David; González-Mancebo, Eloina; Santos Macadán, Sara; Gandolfo Cano, Mar; Pérez-Gordo, Marina; Cases Ortega, Bárbara; Vivanco Martínez, Fernando; Pastor Vargas, CarlosItem New allergen involved in a case of allergy to Solea solea, common sole(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2010) Pérez-Gordo, Marina; Pastor Vargas, Carlos; Cases, Bárbara; De Las Heras, Manuel; Sanz, Aroa; Vivanco Martínez, Fernando; Cuesta-Herranz, JavierItem Allergy to Prairie Dog Lipocalins(ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, 2010) González de Olano, David; Bartolomé, Borja; Cases Ortega, Bárbara; Pérez-Gordo, Marina; Vivanco Martínez, Fernando; Pastor Vargas, CarlosItem Phosphorylation reduces the allergenicity of cow casein in children with selective allergy to goat and sheep milk.(J Investig Allergol Clin Immunol, 2011) Cases, Barbara; García-Ara, Carmen; Boyano, Maria Teresa; Pérez-Gordo, Marina; Pedrosa, Maria; Vivanco Martínez, Fernando; Quirce, Santiago; Pastor Vargas, CarlosThis study aimed to characterize the role of phosphorylation of caseins in selective allergy to goat milk (GM) and sheep milk (SM) in patients with good tolerance to cow milk (CM). We performed skin prick tests with milk and caseins from CM, GM, and SM and immunoblotting and specific immunoglobulin (Ig) E determinations with milk and casein from cow and GM and SM. Sensitization to milk and caseins from goat and sheep was demonstrated in all 3 patients by skin tests, determination of specific IgE, or both. Immunoblotting confirmed that GM/SM proteins but not CM proteins were involved in the allergic symptoms. IgE reacted with several protein bands from the caseins and milk extracts of both sheep and goat. Phosphorylation was involved in the different allergenicity of CM caseins. We report the implication of phosphorylation in the allergenicity of caseins involved in selective allergy to GM and SM.Item Identification of Major Allergens in Watermelon(International Archives on Allergy & Immunology, 2009) Pastor Vargas, Carlos; Cuesta-Herranz, Javier; Cases, Barbara; Pérez-Gordo, Marina; Figueredo, Elena; Heras González, Manuel De Las; Vivanco Martínez, FernandoBackground: Watermelon is a worldwide consumed Cucurbitaceae fruit that can elicit allergic reactions. However, the major allergens of watermelon are not known. The aim of this study is to identify and characterize major allergens in watermelon. Methods: Twenty-three patients allergic to watermelon took part in the study. The diagnosis was based on a history of symptoms and positive skin prick-prick tests to watermelon, confirmed by positive open oral challenge testing to watermelon pulp. Allergenic components were detected by SDS-PAGE and immunoblotting. Molecular characterization of IgE-binding bands was performed by N-terminal amino acid sequencing and mass spectrometry. Allergens were purified combining several chromatographic steps. Results: Several IgE binding bands (8-120 kDa) were detected in watermelon extract. Three major allergens were identified as malate dehydrogenase (36 kDa), triose phosphate isomerase (28 kDa) and profilin (13 kDa). Purified allergens individually inhibited IgE binding to the whole watermelon extract. Conclusions: All in all these results indicate that malate dehydrogenase, triose phosphate isomerase and profilin are major allergens involved in watermelon allergy.Item Allergy to crayfish(J Investig Allergol Clin Immunol, 2011) Gonzalez de Olano, David; Pastor Vargas, Carlos; Gandolfo-Cano, Mar; González-Mancebo, Eloina; Meléndez-Baltanás, Amaya; Morales-Barrios, Pilar; Pérez-Gordo, Marina; Vivanco Martínez, Fernando; Bartolomé, Borja