Person:
Vivanco Martínez, Fernando

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First Name
Fernando
Last Name
Vivanco Martínez
URI
https://hdl.handle.net/20.500.14352/84150
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Area
Bioquímica y Biología Molecular
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2011 - 20142
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Subject: 616-097 ×

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    Allergy to crayfish
    (J Investig Allergol Clin Immunol, 2011) Gonzalez de Olano, David; Pastor Vargas, Carlos; Gandolfo-Cano, Mar; González-Mancebo, Eloina; Meléndez-Baltanás, Amaya; Morales-Barrios, Pilar; Pérez-Gordo, Marina; Vivanco Martínez, Fernando; Bartolomé, Borja
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    Molecular characterization of contact urticaria in patients with melon allergy
    (2014) Gandolfo-Cano, M.; Bartra, J.; González-Mancebo, E.; Feo-Brito, F.; Gómez, E.; Bartolomé, B.; Muñoz-García, E.; Sanz Maroto, A.; Vivanco Martínez, Fernando; Cuesta-Herranz, J.; Pastor Vargas, Carlos
    Background: The relevance of contact allergy to plant-related food has recently emerged. Oral allergy syndrome is one of the most characteristic symptoms of fruit allergy, although it also causes systemic reactions. Plant-food allergy is increasing at the same time as pollen allergy, and fruit-induced allergic contact urticaria could be rising as well. Objectives: The present study was carried out in order to investigate whether one particular primary melon-peel allergen is responsible for contact urticaria. Methods: Fourteen patients presenting with contact urticaria after touching melon peel were evaluated. A melon-peel extract was prepared and analysed by immunoblotting using the patients' sera. Molecular characterization of IgE-binding bands was performed using mass spectrometry. Melon-peel lipid transfer protein (LTP) was purified. Inhibition studies and contact challenge with the protein were performed to confirm IgE reactivity to the purified allergen. Results: An IgE-binding band of ~8-9 kDa was observed in an immunoblotting assay with all the patients' sera and was identified as an LTP. The melon-peel LTP was purified in two chromatography steps. Inhibition studies confirmed LTP as a major allergen in patients with melon-peel contact urticaria. Contact challenge with melon-peel LTP was performed in five patients, all of whom had positive results, exhibiting itchy erythema and hives in the area of contact. Conclusions: This study confirmed our previous findings that melon-peel LTP is a major allergen and is responsible for contact allergy. This knowledge may be used to improve both diagnosis and treatment of patients allergic to melon.