Person:
Sánchez Sánchez, Alfredo

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First Name
Alfredo
Last Name
Sánchez Sánchez
URI
https://hdl.handle.net/20.500.14352/76276
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Analítica
Area
Química Analítica
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2021 - 20233
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Author: Mayol Hornero, Beatriz ×

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Now showing 1 - 3 of 3
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    A glutathione disulfide-sensitive Janus nanomachine controlled by an enzymatic and logic gate for smart delivery
    (Nanoscale, 2021) Mayol Hornero, Beatriz; Díez-Sánchez, Paula; Sánchez Sánchez, Alfredo; Torre, Cristina de la; Villalonga, Anabel; Lucena-Sánchez, Elena; Sancenón, Félix; Martínez Ruiz, María Paloma; Vilela García, Diana; Martínez-Máñez, Ramón; Villalonga Santana, Reynaldo
    This work describes the assembly of a novel enzyme-controlled nanomachine operated through an AND Boolean logic gate for on-command delivery. The nanodevice was constructed on Au-mesoporous silica Janus nanoparticles capped with a thiol-sensitive gate-like molecular ensemble on the mesoporous face and functionalized with glutathione reductase on the gold face. This autonomous nanomachine employed NADPH and glutathione disulfide as input chemical signals, leading to the enzymatic production of reduced glutathione that causes the disruption of the gating mechanism on the mesoporous face and the consequent payload release as an output signal. The nanodevice was successfully used for the autonomous release of doxorubicin in HeLa cancer cells and RAW 264.7 macrophage cells.
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    Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor
    (Biosensors, 2022) Villalonga Chico, Anabel; Parrado Quintela, Concepción; Diaz, Raúl; Sánchez Sánchez, Alfredo; Mayol Hornero, Beatriz; Martínez-Ruíz, Paloma; Vilela García, Diana; Villalonga Santana, Reynaldo
    Here we report a novel labeling strategy for electrochemical aptasensors based on enzymatic marking via supramolecular host–guest interactions. This approach relies on the use of an adamantane-modified target-responsive hairpin DNA aptamer as an affinity bioreceptor, and a neoglycoconjugate of β-cyclodextin (CD) covalently attached to a redox enzyme as a labeling element. As a proof of concept, an amperometric aptasensor for a carcinoembryonic antigen was assembled on screen-printed carbon electrodes modified with electrodeposited fern-like gold nanoparticles/graphene oxide and, by using a horseradish peroxidase-CD neoglycoenzyme as a biocatalytic redox label. This aptasensor was able to detect the biomarker in the concentration range from 10 pg/mL to 1 ng/mL with a high selectivity and a low detection limit of 3.1 pg/mL in human serum samples.
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    Project number: 172
    Mentoría pedagógica como método de formación del profesorado universitario. Experiencia en la asignatura "Química Analítica I" del grado en Química
    (2023) Sánchez Sánchez, Alfredo; Villalonga Santana, Reynaldo; Urraca Ruiz, Javier; Gracia Lor, Emma; Vilela García, Diana; Rodríguez Bencomo, Juan José; Molla Escudero, David; Mayol Hornero, Beatriz; Villalonga Chico, Anabel