Pintos López, Beatriz

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First Name
Last Name
Pintos López
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Biológicas
Genética, Fisiología y Microbiología
Fisiología Vegetal
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Now showing 1 - 4 of 4
  • Publication
    Nanoceria and bulk cerium oxide effects on the germination of Asplenium adiantum-nigrum spores
    (INIA, 2016) Gómez Garay, Aranzazu; Pintos López, Beatriz; Manzanera, José Antonio; Prada Moral, Carmen; Martín Calvarro, Luisa; Gabriel y Galán Moris, José María
    Aim of the study: The effect of cerium oxide engineered nanoparticles on the spore germination of the fern Asplenium adiantumnigrum. Area of study: France, Britanny Region, Finistére Department, Plougonvelin, in rocks near the sea. Material and methods: Asplenium spores were cultured in vitro on agar medium with Nano-CeO2 (less than 25 nm particle size) and bulk-CeO2. The addition of each nano- and bulk particles ranged from 0 to 3000 mg L–1. Observations on rhizoidal and prothallial cells during first stages of gametophyte development were made. The No-Observed-Adverse-Effect concentration (NOAEC) and Lowest-Observed-Adverse-Effect-Concentration (LOEC) values for spore germination rate data were analyzed. Main results: Germination was speeded up by 100 to 2000 mg L–1 nanoceria, while bulk cerium oxide had the same effect for 500 to 2000 mg L–1 concentrations. Present results showed cellular damage in the protonema while rhizoid cells seemed not to be affected, as growth and membrane integrity remained. Research highlights: Both nanosized and bulk cerium oxide are toxic for the fern Asplenium adiantum-nigrum, although diverse toxicity patterns were shown for both materials. Diverse toxic effects have been observed: chloroplast membrane damage and lysis, cell wall and membrane disruption which leads to cell lysis; and alterations in morphology and development.
  • Publication
    Pectin De-methylesterification and AGP Increase Promote Cell Wall Remodeling and Are Required During Somatic Embryogenesis of Quercus suber
    (Frontiers Research Foundation, 2019-01-08) Pérez Pérez, Yolanda; Carneros, Elena; Berenguer, Eduardo; Solís González, María Teresa; Bárány, Ivett; Pintos López, Beatriz; Gómez-Garay, Aranzazu; Risueño, María C.; Testillano, Pilar S.
    Somatic embryogenesis is a reliable system for in vitro plant regeneration, with biotechnological applications in trees, but the regulating mechanisms are largely unknown. Changes in cell wall mechanics controlled by methylesterification of pectins, mediated by pectin methylesterases (PMEs) and pectin methyl esterase inhibitors (PMEIs) underlie many developmental processes. Arabinogalactan proteins (AGPs) are highly glycosylated proteins located at the surface of plasma membranes, in cell walls, and in extracellular secretions, with key roles in a range of different processes. In this study, we have investigated changes in two cell wall components, pectins and AGPs, during somatic embryogenesis in Quercus suber, a forest tree of high economic and ecologic value. At early embryogenesis stages, cells of proembryogenic masses showed high levels of esterified pectins and expression of QsPME and QsPMEI genes encoding a PME and a putative PMEI, respectively. At advanced stages, differentiating cells of heart, torpedo and cotyledonary embryos exhibited walls rich in de-esterified pectins, while QsPME gene expression and PME activity progressively increased. AGPs were detected in cell walls of proembryogenic masses and somatic embryos. QsLys-rich-AGP18, QsLys-rich-AGP17, and QsAGP16L1 gene expression increased with embryogenesis progression, as did the level of total AGPs, detected by dot blot with β-glucosyl Yariv reagent. Immuno dot blot, immunofluorescence assays and confocal analysis using monoclonal antibodies to high- (JIM7, LM20) and low- (JIM5, LM19) methylesterified pectins, and to certain AGP epitopes (LM6, LM2) showed changes in the amount and distribution pattern of esterified/de-esterified pectins and AGP epitopes, that were associated with proliferation and differentiation and correlated with expression of the PME and AGP genes analyzed. Pharmacological treatments with catechin, an inhibitor of PME activity, and Yariv reagent, which blocks AGPs, impaired the progression of embryogenesis, with pectin de-esterification and an increase in AGP levels being necessary for embryo development. Findings indicate a role for pectins and AGPs during somatic embryogenesis of cork oak, promoting the cell wall remodeling during the process. They also provide new insights into the regulating mechanisms of somatic embryogenesis in woody species, for which information is still scarce, opening up new possibilities to improve in vitro embryo production in tree breeding.
  • Publication
    Sap flow, leaf-level gas exchange and spectral responses to drought in Pinus sylvestris, Pinus pinea and Pinus halepensis
    (the Italian Society of Silviculture and Forest Ecology (SISEF), 2016) Manzanera, José Antonio; Gómez Garay, María Aranzazu; Pintos López, Beatriz; Rodríguez Rastrero, Manuel; Moreda, Eugenio; Zazo, Javier; Martínez Falero, Eugenio; García Abril, Antonio
    In a climate change scenario, Mediterranean forest species such as pines may be endangered by rising temperatures and reduced precipitation, thus calling for studies on the transpiration and water balance in pines. In this paper, the response of young plants of Pinus sylvestris L., Pinus pinea L. and Pinus halepensis Mill. to different irrigation treatments has been studied. Significant differences were found in water potential, sap flow, leaf-level gas exchange and spectral variables. P. sylvestris had higher pre-dawn and midday water potentials, sap flow rates and leaf-level gas exchange rates compared to the other two species in well-watered conditions. Vapor pressure gradient correlated with stomatal conductance, net assimilation and transpiration, but the association between stomatal conductance and sap flow was weak. The environmental variables more strongly associated with sap flow were solar radiation and reference evapo-transpiration, especially in the well-watered plants, but those associations were weaker in the stressed plants. All three pine species showed the isohydric, drought-avoiding strategy common in the genus Pinus, maintaining relatively high water potentials in dry conditions. Nevertheless, P. halepensis showed a water-saving strategy, with a stomatal closure behavior under drought. Stomatal regulation was less strict in P. sylvestris, closer to a water-spending pattern, while P. pinea showed an intermediate behavior. Significant differences were recorded among species in spectral reflectance in the visible and infra-red regions. Photochemical Reflectance Index, Normalized Difference Vegetation Index and combinations of other ratios permitted the discrimination among the three pine species. These spectral variables showed association with sap flow rate, water potential and leaflevel gas exchange variables. Both cluster analysis and k-means classification discriminated Scots pine and Aleppo pine in two different groups. On the other hand, Stone pine showed differences in spectral behavior depending on the hydric status of the plants. Well-watered Stone pine plants had the same spectral behavior as Scots pine, while the plants subjected to drought stress were closer to Aleppo pine plants in spectral response. These findings may help to quantify the impacts of early and mid-summer water deficit on Mediterranean pines in future climate regimes.
  • Publication
    ¡No lo tires, aprovéchalo, es Bioeconomía!
    (Universidad Nacional Autónoma de México, Coordinación de Universidad Abierta, Innovación Educativa y Educación a Distancia (CUAIEED), 2023-07-04) Pérez-Urría Carril, Elena; Pintos López, Beatriz; Urrialde De Andrés, Rafael; Gómez Garay, Arancha
    La humanidad tiene por delante grandes retos, entre ellos producir alimentos y energía para una población mundial de casi 8 mil millones de personas. Esta producción debe ser compatible con el mantenimiento y mejora, si cabe, de los medios y entornos naturales o agrícolas. De no ser así, esos medios se agotarán, se destruirán y no habrá posibilidad de producir nada, materias primas —alimentos, materiales o energía— que sostienen nuestras sociedades. Por otra parte, el sector agroalimentario genera multitud de residuos que pueden ser aprovechados para obtener una variedad de productos de alto valor que pueden ser utilizados en agricultura, alimentación o como materia para generar energía. De aquí nace la necesidad de aprovechar los residuos biológicos, los residuos de la agricultura y la alimentación, y contribuir a mejorar los sistemas de producción. La bioeconomía se presenta como una estrategia para alcanzar esa meta.