Agüí Chicharro, María Lourdes

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First Name
María Lourdes
Last Name
Agüí Chicharro
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Química Analítica
Química Analítica
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  • Publication
    An Electrochemical Enzyme Biosensor for 3-Hydroxybutyrate Detection Using Screen-Printed Electrodes Modified by Reduced Graphene Oxide and Thionine
    (MDPI, 2017-11-11) Martínez García, Gonzalo; Pérez Julián, Elena; Agüí Chicharro, María Lourdes; Cabré, Naomí; Joven, Jorge; Yáñez Sedeño, Paloma; Pingarrón Carrazón, José Manuel
    A biosensor for 3-hydroxybutyrate (3-HB) involving immobilization of the enzyme 3-hydroxybutyrate dehydrogenase onto a screen-printed carbon electrode modified with reduced graphene oxide (GO) and thionine (THI) is reported here. After addition of 3-hydroxybutyrate or the sample in the presence of NAD+ cofactor, the generated NADH could be detected amperometrically at 0.0 V vs. Ag pseudo reference electrode. Under the optimized experimental conditions, a calibration plot for 3-HB was constructed showing a wide linear range between 0.010 and 0.400 mM 3-HB which covers the clinically relevant levels for diluted serum samples. In addition, a limit of detection of 1.0 µM, much lower than that reported using other biosensors, was achieved. The analytical usefulness of the developed biosensor was demonstrated via application to spiked serum samples.