Person: Sevane Fernández, Natalia
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First Name
Natalia
Last Name
Sevane Fernández
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Producción Animal
Area
Producción Animal
Identifiers
6 results
Search Results
Now showing 1 - 6 of 6
Item Pedigree analysis of a highly fragmented population, the Lidia cattle breed(Livestock Science, 2014) Cortés Gardyn, Óscar; Sevane Fernández, Natalia; Baro J.A.; Cañón Ferreras, Francisco JavierThe aim of the study was to analyze the pedigree information of the Lidia bovine breed based on animals registered in the Herdbook and belonging to those lineages recognized as conforming to the official breed standard. Pedigree records of 272,574 animals belonging to 83 herds classified in 30 lineages were used. The average number of equivalent generations known was 4.5 (varying among lineages from 4 in Braganza to 5.2 in Baltasar Iban). The generation interval (7.5 years) was longer than that estimated in other cattle breeds. The effective size was less than 50 and consequently the estimated increase in inbreeding per generation was greater than 1% in all the lineages analyzed. The increase in inbreeding level expected for the next 50 years varied from 7.4% in Braganza to 31.3% in Diego Garrido. The ratios among the effective number of founders, the effective number of ancestors and the effective number of founder genomes was considered evidence that genetic drift explained most of the loss of genetic variability in the Lidia bovine breed due to the reduced effective population sizes of the lineages, more than bottlenecks did, as they have been less dramatic. The lineage allele loss due to the genetic drift and the effect of inbreeding are the major concerns in managing the genetic diversity of the Lidia bovine breed. The analysis of pedigree information still remains as the main useful resource to establish genetic diversity conservation guidelines in the Lidia bovine breed. Minimizing inbreeding increase within lineages in the sub-divided Lidia breed must be the major concern in managing the genetic diversity of this breed.Item Dietary inulin supplementation modifies significantly the liver transcriptomic profile of broiler chickens(Plos One, 2014) Sevane Fernández, Natalia; Bialade, Federica; Velasco Villar, Susana; Rebolé Garrigós, Almudena; Rodríguez Membibre, María Luisa; Ortiz Vera, Luis Tomás; Cañón Ferreras, Francisco Javier; Dunner Boxberger, Helene Susana; te Pas Marinus FWInclusion of prebiotics in the diet is known to be advantageous, with positive influences both on health and growth. The current study investigated the differences in the hepatic transcriptome profiles between chickens supplemented with inulin (a storage carbohydrate found in many plants) and controls. Liver is a major metabolic organ and has been previously reported to be involved in the modification of the lipid metabolism in chickens fed with inulin. A nutrigenomic approach through the analysis of liver RNA hybridized to the Affymetrix GeneChip Chicken Genome Array identified 148 differentially expressed genes among both groups: 104 up-regulated (≥1.4-fold) and 44 down-regulated (≤0.6-fold). Quantitative real-time PCR analysis validated the microarray expression results for five out of seven genes tested. The functional annotation analyses revealed a number of genes, processes and pathways with putative involvement in chicken growth and performance, while reinforcing the immune status of animals, and fostering the production of long chain fatty acids in broilers supplemented with 5 g of inulin kg−1 diet. As far as we are aware, this is the first report of a microarray based gene expression study on the effect of dietary inulin supplementation, supporting further research on the use of this prebiotic on chicken diets as a useful alternative to antibiotics for improving performance and general immunity in poultry farming, along with a healthier meat lipid profile.Item A Primer Extension Assay for simultaneous use in cattle Genotype Assisted Selection, Parentage and Traceability analysis(Livestock Science, 2011) Sevane Fernández, Natalia; Cañón Ferreras, Francisco Javier; Dunner Boxberger, Helene Susana; Crespo, IThe use of genotype information as an aid to selection can be a cheap and effective way to improve the genetic progress in beef cattle breeds, specially in the case of high cost phenotypic recording which is true for many economic traits in beef cattle. SNPs located at candidate genes underlying economic traits allow prediction of the genetic merit of individuals and, combined with parentage and traceability analysis, guarantee consumer protection. Here we present a cost-effective technology, the Capillary Primer-Extension Assay, to genotype validated mutations which identify differences between individuals in candidate genes associated directly or potentially with meat tenderness, marbling and muscle growth, milk yield, protein and fat content, sex or coat colour. We genotyped 70 SNPs in 8 beef, 3 dairy and one semi-feral (never selected for any production trait) breeds and present a panel of 53 SNPs with the aim of enabling a reasonable tool for parentage analysis, animal identification and production of markers usable in GAS in small local breeds for which other tools are unaffordable.Item New single nucleotide polymorphisms in Alectoris identified using chicken genome information allow Alectoris introgression detection(Molecular Ecology Resources, 2010) Sevane Fernández, Natalia; Cortés Gardyn, Óscar; García D; Cañón Ferreras, Francisco Javier; Dunner Boxberger, Helene SusanaUsing the chicken genome, 114 polymorphisms (109 SNPs and 5 INDELs) were identified in the Alectoris genus by polymerase chain reaction–single strand conformation polymorphism. Using these, a panel of SNPs is described, which allows easy detection of introgression of Alectoris chukar in wild Alectoris rufa populations, when used with a primer extension protocol. The selected polymorphisms were genotyped and their allelic frequencies estimated on 98 A. rufa partridges sampled from nonrestocking Spanish areas, and 63 A. chukar partridges from Greek and Spanish farms. Power calculations to determine an optimum subset of markers for a given significance level were performed.Item Muscle lipid composition in bulls from 15 European breeds(Livestock Science, 2014) Sevane Fernández, Natalia; Nute G; Sañudo C; Cortés Gardyn, Óscar; Cañón Ferreras, Francisco Javier; Williams JL; Dunner Boxberger, Helene SusanaCattle meat provides essential nutrients necessary for a balanced diet and health preservation. Besides nutritional quality, consumers' preferences are related to specific attributes such as tenderness, taste and flavour. The present study characterizes the fatty acid composition of beef, which is an important factor in both nutritional and quality values, in 15 European cattle breeds fed a similar diet and reared in five countries (United Kingdom, Denmark, France, Italy and Spain). The effect of possible slight differences on diet composition which might have occurred between countries were included in the breed effect which confounds country, diet, slaughter house and slaughter day as all individuals of a same breed were managed simultaneously. The wide range of breeds studied and the significant differences on lipid profile described here provide a broad characterization of beef meat, which allows giving a better response to the variety of consumers' preferences. Regarding meat health benefits, the groups that stand out are: the double-muscled animals, which displayed lower total fat, lower proportion of saturated (SFA) and monounsaturated (MUFA) fatty acids, and a higher proportion of polyunsaturated (PUFA) fatty acids; and Limousin and Charolais breeds with a significantly higher conversion of 18:3n-3 PUFA to the long chain 22:6n-3 PUFA.Item Association of genes involved in carcass and meat quality traits in 15 European bovine breeds(Livestock Science, 2013) Dunner Boxberger, Helene Susana; Sevane Fernández, Natalia; García D; Cortés Gardyn, Óscar; Valentini A; Williams JL; Mangin B; Cañón Ferreras, Francisco Javier; Levéziel HVariations in meat quality traits are under complex genetic control and improvement has been hampered by the difficulty in their measurement. Several QTL have been reported for different meat quality related traits, but few genes have been described which explain large amounts of the phenotypic variation. The use of single nucleotide polymorphism (SNP) marker panels with predictive value for carcass traits have been evaluated for cattle and SNP are commercially available even though their predictive accuracy may be low in different breeds. To identify new molecular markers for meat quality, an association study was performed in 15 breeds of cattle using 389 SNP belonging to 206 candidate genes known to be involved in muscle development, metabolism and structure. Fifty-four SNP belonging to 20 different genes were found associated with different growth, carcass and meat quality traits. Some of them were novel associations and other were replications of known associations. Among the former, the gene-network associated with the calpain/calpastatin system was shown to be associated with meat texture, although small effects are found for the examined polymorphisms. Novel associations also included SNP in AANAT which was associated with collagen (P=0.006), CAST with fatty acid muscle composition (P=0.00003), CYP1A1 with juiciness (P=0.0005), DGAT2 with physical traits (P=0.0009) and lipid content (P=0.01) in muscle, MADH3 with the myofibrilar fragmentation index (MFI) (P=0.01), NEB with weight (P=0.00009), PCSK1 with juiciness (P=0.002), PLOD3 with carcass performance (P=0.0009) and fatty acids (P=0.04), and PGAM2 and VIM with post-mortem maturation (P=0.00008 and 0.000005, respectively). These data provide a starting point to investigate the complex gene-networks underlying economically important traits which are of importance to the beef industry for the improvement of production efficiency and meat quality.