Person: Crespo Castejón, Francisco
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First Name
Francisco
Last Name
Crespo Castejón
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Veterinaria
Department
Medicina y Cirugía Animal
Area
Medicina y Cirugía Animal
Identifiers
4 results
Search Results
Now showing 1 - 4 of 4
- Project number: 36
Item Modelo docente de palpación del aparato reproductor en bovino como alternativa al uso de materiales de prácticasblanco(2023) Sánchez Calabuig, María Jesús; Blanco Murcia, Francisco Javier; Moreno Gonzalo, Javier; Fominaya García, Hernán Luis; Zanitoni, Morgane; Serres Dalmau, María Consolacion; Crespo Castejón, Francisco; Domínguez Gimbernat, Mónica; Gutiérrez Cepeda, Luna; Mejías López, Elena; Sánchez Calabuig, María Jesús Item Strategies to Reduce the Use of Antibiotics in Fresh and Chilled Equine Semen(Animals, 2024) Zabala Argüelles, Sonsoles Mercedes; Serres Dalmau, María Consolacion; Montero Serra, Natalia; Crespo Castejón, Francisco; Lorenzo González, Pedro Luis; Pérez-Aguilera, Verónica; Galán López, Carmen; Domínguez Gimbernat, Mónica; Oliet Palá, Agustín; Moreno, Santiago; González Zorn, Bruno; Gutiérrez Cepeda, LunaThe study assessed the impact of four equine semen processing techniques on sperm quality and microbial load immediately post-processing and after 48 h of refrigeration. The aim was to explore the potential reduction of prophylactic antibiotic usage in semen extenders. Semen from ten adult stallions was collected and processed under a strict hygiene protocol and divided into four aliquots: Simple Centrifugation with antibiotics (SC+), Simple Centrifugation (SC−), Single-Layer Colloidal Centrifugation (CC−), and Filtration (with SpermFilter®) (F−), all in extenders without antibiotics. Sperm motility, viability, and microbial load on three culture media were assessed. No significant differences were observed in the main in the sperm quality parameters among the four protocols post-processing and at 48 h (p < 0.05 or p < 0.1). Microbial loads in Columbia 5% Sheep Blood Agar and Schaedler vitamin K1 5% Sheep Blood Agar mediums were significantly higher (p < 0.10) for raw semen than for CS+, CC−, and F− post-processing. For Sabouraud Dextrose Agar medium, the microbial load was significantly higher (p < 0.10) in raw semen compared to CS+ and F−. No significant differences (p < 0.10) were found in 48 h chilled samples. Regardless of antibiotic presence, the evaluated processing methods, when combined with rigorous hygiene measures, maintained semen quality and reduced microbial load to the same extent as a traditional protocol using antibiotics.Item Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation(Animals (based), 2022) Bláquez Sarro, Juan Carlos; Gutiérrez Cepeda, Luna; Crespo Castejón, Francisco; Serres Dalmau, María ConsolacionThe Purebred Spanish Horse, according to our clinical experience, is characterized by having a high number of stallions that do not meet the international commercial recommendations forequine-sperm cryopreservation. This means that artificial insemination with frozen semen from these stallions is less widespread than in other breeds. In this study, we investigated if the incorporation ofsingle-layer colloidal centrifugation prior to cryopreservation in clinical conditions could increase the number of ejaculates of Purebred Spanish stallions suitable for this processing, observing the influence of centrifugation and freezing extender protocol on post-thawed sperm motility. Using colloidal centrifugation, the percentage of ejaculates available to be frozen was increased from 35% (6/17) to71% (12/17), doubling the number of samples that could have been subjected to cryopreservation. We only found significant differences in linearity (LIN) and lateral head displacement (ALH) after5 min of incubation at 37 ◦C between colloidal and simple centrifugation processing techniques. No significant differences were found between the two different colloidal protocols in any of the variables considered. Colloidal centrifugation allowed us to obtain, from worse fresh-quality ejaculates, thawed sperm doses with similar quality to that of good-quality ejaculates. BotuCrio® produced, in general higher motility parameters and its characteristics than the other extenders analyzed, with significant differences found in comparison to Inra-Freeze® and Lac-Edta in both total (MOT) and progressive motility (PMOT) when using colloidal centrifugation and only in PMOT when applying simple centrifugation. Colloidal centrifugation optimized the efficiency of cryopreservation, as it allowed usto increase the number of ejaculates of Purebred Spanish Horses suitable to be frozen. Including these semen processing techniques in the freeze test could help to optimize equine-sperm cryopreservation protocols, especially when dealing with individuals or breeds for which initially low sperm quality prevents or limits their inclusion in sperm cryopreservation programs- Project number: 382
Item Elaboración de material docente para la enseñanza de la exploración física del caballo(2022) Fores Jackson, Paloma; Gutiérrez Cepeda, Luna; Villaescusa Fernández, Alejandra; Villalba Orero, María; Ruiz de León Robledo, María de los Ángeles; Crespo Castejón, Francisco; Dominuez Gimbernat, MonicaEl objetivo principal del presente proyecto fue incorporar una serie de recursos educativos virtuales que facilitaran e implementaran la enseñanza de la exploración física del caballo.