Person:
García Oliva, Cecilia María

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First Name
Cecilia María
Last Name
García Oliva
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Farmacia
Department
Química en Ciencias Farmacéuticas
Area
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UCM identifierScopus Author IDDialnet ID

Search Results

Now showing 1 - 9 of 9
  • Publication
    Desarrollo y elaboración de recursos didácticos on line para fomentar el autoaprendizaje interactivo y la autoevaluación en Química Farmacéutica con el apoyo del Campus Virtual
    (2018-06-30) Hoyos Vidal, Pilar; Hernáiz Gómez-Degano, María Josefa; Izquierdo Jiménez, Inmaculada; Alemán Sierra, Esther; García Oliva, Cecilia María
    En este Proyecto de Innovación Docente se ha pretendido de manera general optimizar las alternativas ofrecidas en el Campus Virtual, mediante el diseño, elaboración y evaluación de actividades didáticas on-line que permitan que el Aula Virtual se convierta en un apoyo activo en el proceso enseñanza-aprendizaje en la asignatura de Química Farmacéutica II. Se ha llevado a cabo la elaboración de cuestionarios interactivos de autoevaluación que permitan al alumno conocer la adecuación de los conocimientos adquiridos, y al docente poder realizar un seguimiento rápido de cada alumno.
  • Publication
    Abordaje terapéutico del alcoholismo
    (2015-06) García Oliva, Cecilia María; Martín-Aragón, Sagrario
    El consumo de alcohol es una práctica muy extendida y socialmente aceptada en nuestro entorno; sin embargo, se trata del tercer factor de riesgo más importante para la salud. Las consecuencias derivadas del abuso del alcohol van más allá de la aparición de enfermedades, ya que conlleva una gran carga social y económica. El metabolismo del etanol ocasiona un exceso de NADH, por lo que disminuye la cantidad de cofactor oxidado disponible para otras reacciones, alterando numerosas rutas bioquímicas. Un consumo crónico ocasiona la desregulación del sistema nervioso central, adaptándose a la presencia de alcohol en el organismo. Si se cesa el consumo aparece un síndrome de abstinencia como consecuencia del desequilibrio entre los distintos neurotransmisores ante la falta de alcohol. La terapia psicológica es indispensable antes, durante y después del tratamiento farmacológico, ya que el paciente es el que debe tomar la decisión de dejar la bebida, ser consciente de su enfermedad y responsabilizarse de la adherencia al tratamiento. El primer paso en la terapia farmacológica es prevenir de la aparición del síndrome de abstinencia o disminuir sus síntomas, fundamentalmente se emplean fármacos sedantes. Una vez superada esta fase aún se mantiene el recuerdo de la sensación placentera asociada al consumo, por lo que los fármacos empleados se dirigen a prolongar la abstinencia y evitar recaídas. Un nuevo fármaco, llamado nalmefeno, permite la disminución gradual del consumo evitando la completa pérdida de control. La eficacia del tratamiento no siempre es elevada, debido fundamentalmente a las numerosas variaciones genéticas entre individuos; determinan tanto la predisposición al alcoholismo como la respuesta ante los distintos fármacos. El tratamiento ideal estaría fundamentado en la elección individualizada de los fármacos en función de las características genéticas y situación personal del paciente.
  • Publication
    Aula virtual para el aprendizaje práctico de la resolución enzimática de ácidos 2-arilpropiónicos usando estudios computacionales (Docking), hidrólisis enzimática y resonancia magnética nuclear (RMN)
    (2022-09-27) Perona Requena, Almudena; Hoyos Vidal, María Pilar; Ramírez López, Pedro; Aguilar-Amat, Aida Flores; Martínez Espinosa, Carlos Antonio; García Oliva, Cecilia María; Merchán del Real, Alejandro; Hadri, Nada; Jiménez Sánchez, Ana; Iniesta Meco, Juan
    Aula virtual para el alumnado de Química Farmacéutica II y Biotecnología Farmacéutica II, en la cual, mediante elementos multimedia, aprenderán de forma autónoma a realizar la práctica de la resolución enzimática de ácidos 2-arilpropiónicos. En el aula virtual podrán aprender el uso de técnicas computacionales como el docking, así como a realizar reacciones enzimáticas y a interpretar el resultado de la reacción mediante resonancia magnética nuclear. Se usarán recursos educativos abiertos y enseñanza virtual.
  • Publication
    Acceptor Specificity of β-N-Acetylhexosaminidase from Talaromyces flavus: A Rational Explanation
    (MDPI, 2019-12-07) García Oliva, Cecilia María; Hoyos Vidal, Pilar; Petrásková, Lucie; Kulik, Natalia; Pelantová, Helena; Cabanillas, Alfredo H.; Rumbero, Ángel; Křen, Vladimír; Hernáiz Gómez-Dégano, María José; Bojarová, Pavla
    Fungal β-N-acetylhexosaminidases, though hydrolytic enzymes in vivo, are useful tools in the preparation of oligosaccharides of biological interest. The β-N-acetylhexosaminidase from Talaromyces flavus is remarkable in terms of its synthetic potential, broad substrate specificity, and tolerance to substrate modifications. It can be heterologously produced in Pichia pastoris in a high yield. The mutation of the Tyr470 residue to histidine greatly enhances its transglycosylation capability. The aim of this work was to identify the structural requirements of this model β-N-acetylhexosaminidase for its transglycosylation acceptors and formulate a structure–activity relationship study. Enzymatic reactions were performed using an activated glycosyl donor, 4-nitrophenyl N-acetyl-β-d-glucosaminide or 4-nitrophenyl N-acetyl-β-d-galactosaminide, and a panel of glycosyl acceptors of varying structural features (N-acetylglucosamine, glucose, N-acetylgalactosamine, galactose, N-acetylmuramic acid, and glucuronic acid). The transglycosylation products were isolated and structurally characterized. The C-2 N-acetamido group in the acceptor molecule was found to be essential for recognition by the enzyme. The presence of the C-2 hydroxyl moiety strongly hindered the normal course of transglycosylation, yielding unique non-reducing disaccharides in a low yield. Moreover, whereas the gluco-configuration at C-4 steered the glycosylation into the β(1-4) position, the galacto-acceptor afforded a β(1-6) glycosidic linkage. The Y470H mutant enzyme was tested with acceptors based on β-glycosides of uronic acid and N-acetylmuramic acid. With the latter acceptor, we were able to isolate and characterize one glycosylation product in a low yield. To our knowledge, this is the first example of enzymatic glycosylation of an N-acetylmuramic acid derivative. In order to explain these findings and predict enzyme behavior, a modeling study was accomplished that correlated with the acquired experimental data.
  • Publication
    Virtualización de la docencia práctica de Química Orgánica (I y II) en el Grado en Farmacia y Doble Grado de en Farmacia y Nutrición Humana y Dietética
    (2021-09) Blázquez Barbadillo, Cristina; Carmona Zafra, Noelia; Cledera Crespo, María del Pilar; Fernández Fernández, María; García Oliva, Cecilia María; Giorgi, Giorgio; León Martínez, Rafael; López-Alvarado Gutiérrez, María del Pilar; Muñoz Silva, Miguel; Peñas Merino, María Eugenia; Perez Moreno, José Miguel; Perona Requena, Almudena; Ramírez López, Pedro; Ramos García, María Teresa; Rodríguez Cordero, Maria Luisa; Ruiz Serrano, Miriam; Sanchez Cebrián, Juan Domingo; Sánchez Montero, José; Sarabia Vallejo, Alvaro; Sollhuber Kretzer, Mónica
    El presente proyecto recoge las acciones llevadas a cabo para virtualizar en el campus virtual las prácticas de las asignaturas de Química Organica (I y II), con el fin de mejorar la comprensión del alumnado de las mismas. Se han realizado presentaciones en Power Point de los fundamentos de cada una de las prácticas así como un video demostrativo de su realización.
  • Publication
    Efficient Synthesis of Muramic and Glucuronic Acid Glycodendrimers as Dengue Virus Antagonists
    (2020-02-03) Cabanillas, Alfredo H; Rumbero, Ángel; García Oliva, Cecilia María; Perona Requena, Almudena; Hernáiz Gómez-Degano, María Josefa; Hoyos Vidal, María Pilar
    Carbohydrates are involved in many important pathological processes, such as bacterial and viral infections, by means of carbohydrate-protein interactions. Glycoconjugates with multiple carbohydrates are involved in multivalent interactions, thus increasing their binding strengths to proteins. In this work, we report the efficient synthesis of novel muramic and glucuronic acid glycodendrimers as potential Dengue virus antagonists. Aromatic scaffolds functionalized with a terminal ethynyl groups were coupled to muramic and glucuronic acid azides by click chemistry through optimized synthetic strategies to afford the desired glycodendrimers with high yields. Surface Plasmon Resonance studies have demonstrated that the compounds reported bind efficiently to the Dengue virus envelope protein. Molecular modelling studies were carried out to simulate and explain the binding observed. These studies confirm that efficient chemical synthesis of glycodendrimers can be brought about easily offering a versatile strategy to find new active compounds against Dengue virus.
  • Publication
    Development of sustainable synthesis of glucuronic acid glycodendrimers using ball milling and microwave-assisted CuAAC reaction
    (Royal Society of Chemistry, 2022-03-08) García Oliva, Cecilia María; Merchán, Alejandro ; Perona Requena, Almudena; Hoyos Vidal, María Pilar; Rumbero, Ángel ; Hernáiz Gómez-Degano, María Josefa
    Two green strategies for copper-catalyzed azide-alkyne cycloaddition reaction based on two activation pathways, solvent free mechanochemistry and microwave irradiation using a recycable biosolvent, are reported for the synthesis of glucuronic acid glycodendrimers with good conversions (99%), the MW pathway being the ideal tool (90% yield in 90 min).
  • Publication
    Enzymatic Synthesis and Molecular Modelling Studies of Rhamnose Esters Using Lipase from Pseudomonas stutzeri
    (Multidisciplinary Digital Publishing Institute (MDPI), 2022-02-17) Perona Requena, Almudena; García Oliva, Cecilia María; Rumbero, Ángel; Hoyos Vidal, María Pilar; Hernáiz Gómez-Degano, María Josefa; Hernáiz Gómez-Degano, María Josefa
    Rhamnolipids are becoming an important class of glycolipid biosurfactants. Herein, we describe for the first time the enzymatic synthesis of rhamnose fatty acid esters by the transesterification of rhamnose with fatty acid vinyl esters, using lipase from Pseudomonas stutzeri as a biocatalyst. The use of this lipase allows excellent catalytic activity in the synthesis of 4-O-acylrhamnose (99% conversion and full regioselectivity) after 3 h of reaction using tetrahydrofuran (THF) as the reaction media and an excess of vinyl laurate as the acyl donor. The role of reaction conditions, such as temperature, the substrates molar ratio, organic reaction medium and acyl donor chain-length, was studied. Optimum conditions were found using 35 °C, a molar ratio of 1:3 (rhamnose:acyldonor), solvents with a low logP value, and fatty acids with chain lengths from C4 to C18 as acyl donors. In hydrophilic solvents such as THF and acetone, conversions of up to 99–92% were achieved after 3 h of reaction. In a more sustainable solvent such as 2-methyl-THF (2-MeTHF), high conversions were also obtained (86%). Short and medium chain acyl donors (C4–C10) allowed maximum conversions after 3 h, and long chain acyl donors (C12–C18) required longer reactions (5 h) to get 99% conversions. Furthermore, scaled up reactions are feasible without losing catalytic action and regioselectivity. In order to explain enzyme regioselectivity and its ability to accommodate ester chains of different lengths, homology modelling, docking studies and molecular dynamic simulations were performed to explain the behaviour observed.
  • Publication
    Enzymatic Synthesis and Molecular Modelling Studies of Rhamnose Esters Using Lipase from Pseudomonas stutzeri
    (Multidisciplinary Digital Publishing Institute (MDPI), 2022-02-17) Hernáiz Gómez-Degano, María Josefa; Perona Requena, Almudena; Rumbero, Ángel ; Hoyos Vidal, María Pilar; García Oliva, Cecilia María; Hernáiz Gómez-Degano, María Josefa
    Rhamnolipids are becoming an important class of glycolipid biosurfactants. Herein, we describe for the first time the enzymatic synthesis of rhamnose fatty acid esters by the transesterification of rhamnose with fatty acid vinyl esters, using lipase from Pseudomonas stutzeri as a biocatalyst. The use of this lipase allows excellent catalytic activity in the synthesis of 4-O-acylrhamnose (99% conversion and full regioselectivity) after 3 h of reaction using tetrahydrofuran (THF) as the reaction media and an excess of vinyl laurate as the acyl donor. The role of reaction conditions, such as temperature, the substrates molar ratio, organic reaction medium and acyl donor chain-length, was studied. Optimum conditions were found using 35 °C, a molar ratio of 1:3 (rhamnose:acyldonor), solvents with a low logP value, and fatty acids with chain lengths from C4 to C18 as acyl donors. In hydrophilic solvents such as THF and acetone, conversions of up to 99–92% were achieved after 3 h of reaction. In a more sustainable solvent such as 2-methyl-THF (2-MeTHF), high conversions were also obtained (86%). Short and medium chain acyl donors (C4–C10) allowed maximum conversions after 3 h, and long chain acyl donors (C12–C18) required longer reactions (5 h) to get 99% conversions. Furthermore, scaled up reactions are feasible without losing catalytic action and regioselectivity. In order to explain enzyme regioselectivity and its ability to accommodate ester chains of different lengths, homology modelling, docking studies and molecular dynamic simulations were performed to explain the behaviour observed.