Person:
Candel González, Francisco Javier

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First Name
Francisco Javier
Last Name
Candel González
URI
https://hdl.handle.net/20.500.14352/79145
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Medicina
Area
Microbiología
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2022 - 20243
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Author: Anguita Mandly, Eduardo Luis × Start date: 2004 ×

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Now showing 1 - 3 of 3
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    Tigecycline Opposes Bortezomib Effect on Myeloma Cells Decreasing Mitochondrial Reactive Oxygen Species Production
    (International journal of molecular sciences, 2024) Ramos Acosta, Carlos; Huerta Pantoja, Laura; Salazar Hidalgo, Milton Eduardo; Mayol, Elsa; Jiménez Vega, Selene; García Peña, Pablo; Jordi Cruz, Jenifeer; Baquero, Cristina; Porras Gallo, María Almudena; Íñigo Rodríguez, Belén; Benavente Cuesta, Celina; Candel González, Francisco Javier; Anguita Mandly, Eduardo Luis
    Multiple myeloma is an incurable plasma cell malignancy. Most patients end up relapsing and developing resistance to antineoplastic drugs, like bortezomib. Antibiotic tigecycline has activity against myeloma. This study analyzed tigecycline and bortezomib combination on cell lines and plasma cells from myeloma patients. Apoptosis, autophagic vesicles, mitochondrial mass, mitochondrial superoxide, cell cycle, and hydrogen peroxide were studied by flow cytometry. In addition, mitochondrial antioxidants and electron transport chain complexes were quantified by reverse transcription real-time PCR (RT-qPCR) or western blot. Cell metabolism and mitochondrial activity were characterized by Seahorse and RT-qPCR. We found that the addition of tigecycline to bortezomib reduces apoptosis in proportion to tigecycline concentration. Supporting this, the combination of both drugs counteracts bortezomib in vitro individual effects on the cell cycle, reduces autophagy and mitophagy markers, and reverts bortezomib-induced increase in mitochondrial superoxide. Changes in mitochondrial homeostasis and MYC upregulation may account for some of these findings. These data not only advise to avoid considering tigecycline and bortezomib combination for treating myeloma, but caution on the potential adverse impact of treating infections with this antibiotic in myeloma patients under bortezomib treatment.
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    Biomarkers of stable and decompensated phases of heart failure with preserved ejection fraction
    (International journal of cardiology, 2022) Anguita Mandly, Eduardo Luis; Chaparro, Alberto; Candel González, Francisco Javier; Ramos Acosta, Carlos; Torrejón, María José; Llopis García, Guillermo; Suárez Cadenas, María del Mar; Matesanz, Mayra; González Del Castillo, Juan María; Martín Sánchez, Francisco Javier; Anguita, Eduardo
    Background Heart failure with preserved ejection fraction (HFpEF) is a disorder related to patient comorbidities and aging. Whether mitochondrial dysfunction is present during HFpEF decompensation versus the stable phase is largely unknown. The aim of the present study was to identify mitochondrial and cell metabolism blood biomarkers in older patients with acute and stable HFpEF. Methods Peripheral blood biomarkers were investigated in a group of eight to 12 patients aged 80–96 years and diagnosed with HFpEF first when they were in decompensated phase and then at least three months later in stable phase. Their data were compared to two control groups with an equal number of participants and sex proportions. One group was age matched and the other included individuals aged between 22 and 44 years. Results Decompensated patients experienced an increased mitochondrial superoxide production and mitochondrial mass, lower mitochondrial DNA copy number and LDHB expression, and higher lactate level compared to the stable stage. The stable phase was characterized by a sharp reduction in formate level. Multivariate analysis indicated that formate, lactate, and histidine can distinguish both of the HFpEF phases. Many of these parameters, including LDHB, lactate, formate, and mitochondrial mass, followed an age-related pattern, with acute HFpEF at its apex or nadir, suggesting that it represents an exacerbation of an aging-related process. Conclusions We identified distinct blood biomarkers of chronic and decompensated HFpEF phases. The data underlined the relationship between HFpEF and aging. These findings could be used to monitor patients and might be therapeutically targeted.
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    Project number: 9
    Participa en la UCM
    (2024) Collado Yurrita, Luis Rodolfo; Ciudad Cabañas, María José; Llama Palacios, María Arantxazu; Sánchez Beltrán, María Del Carmen; Burillo Albizua, Almudena; Andrés Belmonte, Amado; Anguita Mandly, Eduardo Luis; Delgado Vázquez, Rafael; Larrainzar Garijo, Ricardo; Izquierdo Ramiro, Eva; Marin Viuecho, Julia; Oreja Guevara, Celia; Puente Maestu, Luis; Río Gallegos, Francisco José Del; Serra Rexach, José Antonio; Vidán Astiz, María Teresa; Candel González, Francisco Javier; García Alfonso, María Pilar; Casero Mersich, Alba Piroska; Espa Charlan, Laura; Collado Yurrita, Luis Rodolfo
    El Proyecto presentado "Participa en la UCM". Es un Proyecto de innovación Docente, cuyo objetivo principal era evaluar el grado de conocimiento, por parte de los estudiantes de los primeros cursos ( 1º y 2º)de diferentes grados de Ciencias de la Salud, básicamente Odontología, Medicina y Nutrición Humana y Dietetica, de las estructuras organizativas y el funcionamiento de la Universidad Complutense de Madrid (UCM) El proyecto se realizo en tres fases durante el curso 2023/24. En la primera de ellas se evaluó, mediante encuestas verbales y personales el grado de conocimiento de los estudiantes de las estructuras organizativas y del funcionamiento de la Universidad Complutense de Madrid. En una segunda fase se realizaron talleres formativos para explicar dichas estructuras y órganos de gobierno de la UCM y las diferentes formas de participación en las que podrían involucrarse r los estudiantes en estas estructuras institucionales. Posteriormente , y por ultimo se realizo una nueva encuesta verbal y personalizada por ver el grado de conocimientos adquiridos y el posible interés futuro de participar como estudiantes en las estructuras universitarias. Como resultado del Proyecto, podemos afirmar que existe un importante desconocimiento, por parte de los estudiantes, de las estructuras organizativas de la universidad, que no solo se circunscriben a la UCM y que en nuestra opinión, deberían desarrollarse las herramientas, necesarias, por parte de las Instituciones de Educación Superior, para implementar de forma mas amplia el conocimiento e interés de los estudiantes , de las Estructuras, Funcionamiento y Órganos de Gobierno de sus Universidad, y que no vean estas como un simple lugar donde estas ubicados hasta obtener un titulo que les habilite a un futuro ejercicio profesional.