Person:
López Montero, Iván

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First Name
Iván
Last Name
López Montero
URI
https://hdl.handle.net/20.500.14352/76271
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Física
Area
Química Física
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2020 - 20234
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Author: Almendro Vedia, Víctor Galileo × Item Type: Publication ×

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Now showing 1 - 5 of 5
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    Expansion microscopy applied to mono- and dual-species biofilms
    (Biofilms and Microbiomes, 2023) Valdivieso González, David; Jara Pérez, Josué; Almendro Vedia, Víctor Galileo; Orgaz Martín, Belén; López Montero, Iván
    Expansion microscopy (ExM) is a new super-resolution technique based on embedding the biological sample within a hydrogel and its physical expansion after swelling. This allows increasing its size by several times while preserving its structural details. Applied to prokaryotic cells, ExM requires digestion steps for efficient expansion as bacteria are surrounded by a rigid cell wall. Furthermore, bacteria can live in social groups forming biofilms, where cells are protected from environmental stresses by a self-produced matrix. The extracellular matrix represents an additional impenetrable barrier for ExM. Here we optimize the current protocols of ExM and apply them to mono- and dual-species biofilms formed by clinical isolates of Limosilactobacillus reuteri, Enterococcus faecalis, Serratia marcescens and Staphylococcus aureus. Using scanning electron microscopy for comparison, our results demonstrate that embedded bacteria expanded 3-fold. Moreover, ExM allowed visualizing the three-dimensional architecture of the biofilm and identifying the distribution of different microbial species and their interactions. We also detected the presence of the extracellular matrix after expansion with a specific stain of the polysaccharide component. The potential applications of ExM in biofilms will improve our understanding of these complex communities and have far-reaching implications for industrial and clinical research.
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    Project number: 224
    Entorno multimedia y OpenSource para la enseñanza de la Física con material interactivo
    () Almendro Vedia, Víctor Galileo; Fortún García, Adelia; López Montero, Iván; Valdivieso González, David
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    The GDP-Bound State of Mitochondrial Mfn1 Induces Membrane Adhesion of Apposing Lipid Vesicles through a Cooperative Binding Mechanism
    (Biomolecules, 2020) Tolosa Díaz, Andrés; Almendro Vedia, Víctor Galileo; Natale, Paolo; López Montero, Iván
    Mitochondria are double-membrane organelles that continuously undergo fission and fusion. Outer mitochondrial membrane fusion is mediated by the membrane proteins mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2), carrying a GTP hydrolyzing domain (GTPase) and two coiled-coil repeats. The detailed mechanism on how the GTP hydrolysis allows Mfns to approach adjacent membranes into proximity and promote their fusion is currently under debate. Using model membranes built up as giant unilamellar vesicles (GUVs), we show here that Mfn1 promotes membrane adhesion of apposing lipid vesicles. The adhesion forces were sustained by the GDP-bound state of Mfn1 after GTP hydrolysis. In contrast, the incubation with the GDP:AlF− 4 , which mimics the GTP transition state, did not induce membrane adhesion. Due to the flexible nature of lipid membranes, the adhesion strength depended on the surface concentration of Mfn1 through a cooperative binding mechanism. We discuss a possible scenario for the outer mitochondrial membrane fusion based on the modulated action of Mfn1.
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    Tunable gold nanorod/NAO conjugates for selective drug delivery in mitochondria-targeted cancer therapy
    (Nanoscale, 2022) González-Rubio, Sergio; Salgado, Cástor; Manzaneda González, Vanesa; Muñoz Úbeda, Mónica; Ahijado Guzmán, Rubén; Natale, Paolo; Almendro Vedia, Víctor Galileo; Junquera González, María Elena; Osío Barcina, José De Jesús; Ferrer, Irene; Guerrero Martínez, Andrés; Paz-Ares Rodríguez, Luis Gonzaga; López Montero, Iván
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    Polar ammoniostyryls easily converting a clickable Q1 lipophilic BODIPY in an advanced plasma membrane probe†
    (Journal of Materials Chemistry B, 2023) Serrano-Buitrago, Sergio; Muñoz Úbeda, Mónica; Almendro Vedia, Víctor Galileo; Sánchez-Camacho, Juan; Lora Maroto, Beatriz; Moreno, Florencio; Bañuelos, Jorge; García-Moreno, Inmaculada; López Montero, Iván; Moya Cerero, Santiago De La; Moreno Jiménez, Florencio
    A very simple, small and symmetric, but highly bright, photostable and functionalizable molecular probe for plasma membrane (PM) has been developed from an accessible, lipophilic and clickable organic dye based on BODIPY. To this aim, two lateral polar ammoniostyryl groups were easily linked to increase the amphiphilicity of the probe and thus its lipid membrane partitioning. Compared to the BODIPY precursor, the transversal diffusion across lipid bilayers of the ammoniostyryled BODIPY probe was highly reduced, as evidenced by fluorescence confocal microscopy on model membranes built up as giant unilamellar vesicles (GUVs). Moreover, the ammoniostyryl groups endow the new BODIPY probe with the ability to optically work (excitation and emission) in the bioimaging-useful red region, as shown by staining of the plasma membrane of living mouse embryonic fibroblasts (MEFs). Upon incubation, this fluorescent probe rapidly entered the cell through the endosomal pathway. By blocking the endocytic trafficking at 4 °C, the probe was confined within the PM of MEFs. Our experiments show the developed ammoniostyrylated BODIPY as a suitable PM fluorescent probe, and confirm the synthetic approach for advancing PM probes, imaging and science.