Person: López Montero, Iván
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First Name
Iván
Last Name
López Montero
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Ciencias Químicas
Department
Química Física
Area
Química Física
Identifiers
4 results
Search Results
Now showing 1 - 4 of 4
Item Tunable gold nanorod/NAO conjugates for selective drug delivery in mitochondria-targeted cancer therapy(Nanoscale, 2022) González-Rubio, Sergio; Salgado, Cástor; Manzaneda González, Vanesa; Muñoz Úbeda, Mónica; Ahijado Guzmán, Rubén; Natale, Paolo; Almendro Vedia, Víctor Galileo; Junquera González, María Elena; Osío Barcina, José De Jesús; Ferrer, Irene; Guerrero Martínez, Andrés; Paz-Ares Rodríguez, Luis Gonzaga; López Montero, IvánItem Polar ammoniostyryls easily converting a clickable Q1 lipophilic BODIPY in an advanced plasma membrane probe†(Journal of Materials Chemistry B, 2023) Serrano-Buitrago, Sergio; Muñoz Úbeda, Mónica; Almendro Vedia, Víctor Galileo; Sánchez-Camacho, Juan; Lora Maroto, Beatriz; Moreno, Florencio; Bañuelos, Jorge; García-Moreno, Inmaculada; López Montero, Iván; Moya Cerero, Santiago De La; Moreno Jiménez, FlorencioA very simple, small and symmetric, but highly bright, photostable and functionalizable molecular probe for plasma membrane (PM) has been developed from an accessible, lipophilic and clickable organic dye based on BODIPY. To this aim, two lateral polar ammoniostyryl groups were easily linked to increase the amphiphilicity of the probe and thus its lipid membrane partitioning. Compared to the BODIPY precursor, the transversal diffusion across lipid bilayers of the ammoniostyryled BODIPY probe was highly reduced, as evidenced by fluorescence confocal microscopy on model membranes built up as giant unilamellar vesicles (GUVs). Moreover, the ammoniostyryl groups endow the new BODIPY probe with the ability to optically work (excitation and emission) in the bioimaging-useful red region, as shown by staining of the plasma membrane of living mouse embryonic fibroblasts (MEFs). Upon incubation, this fluorescent probe rapidly entered the cell through the endosomal pathway. By blocking the endocytic trafficking at 4 °C, the probe was confined within the PM of MEFs. Our experiments show the developed ammoniostyrylated BODIPY as a suitable PM fluorescent probe, and confirm the synthetic approach for advancing PM probes, imaging and science.Item Transgene expression in mice of the Opa1 mitochondrial transmembrane protein through bicontinuous cubic lipoplexes containing gemini imidazolium surfactants(Journal of Nanobiotechnology, 2021) Muñoz Úbeda, Mónica; Semenzato, Martina; Franco-Romero, Anais; Junquera González, María Elena; Aicart Sospedra, Emilio; Scorrano, Luca; López Montero, IvánLipoplexes are non-viral vectors based on cationic lipids used to deliver DNA into cells, also known as lipofection. The positively charge of the hydrophilic head-group provides the cationic lipids the ability to condensate the negatively charged DNA into structured complexes. The polar head can carry a large variety of chemical groups including amines as well as guanidino or imidazole groups. In particular, gemini cationic lipids consist of two positive polar heads linked by a spacer with different length. As for the hydrophobic aliphatic chains, they can be unsaturated or saturated and are connected to the polar head-groups. Many other chemical components can be included in the formulation of lipoplexes to improve their transfection efficiency, which often relies on their structural features. Varying these components can drastically change the arrangement of DNA molecules within the lamellar, hexagonal or cubic phases that are provided by the lipid matrix. Lipofection is widely used to deliver genetic material in cell culture experiments but the simpler formulations exhibit major drawbacks related to low transfection, low specificity, low circulation half-life and toxicity when scaled up to in vivo experiments.Item Dissimilar-at-boron N-BODIPYs: from light-harvesting multichromophoric arrays to CPL-bright chiral-at-boron BODIPYs(Organic Chemistry Frontiers, 2023) Ray, César; Avellanal-Zaballa, Edurne; Muñoz Úbeda, Mónica; Colligan, Jessica; Moreno Jiménez, Florencio; Muller, Gilles ; López Montero, Iván; Bañuelos, Jorge; Lora Maroto, Beatriz; Moya Cerero, Santiago De LaWe report a workable and easy approach for the direct post-multifunctionalization of common BODIPYs (F-BODIPYs) with minimal interference to the starting photophysical behavior. It entails the easy transformation of an F-BODIPY into the corresponding N-BODIPY by using a dissimilarly-N,N′-disubstituted bis(sulfonamide), which is easily obtained from ethane-1,2-diamine. This approach is exemplified by the rapid synthesis of a selected battery of unprecedented dissimilar-at-boron N-BODIPYs, which are rationally designed to act as efficient multichromophoric arrays for light harvesting by excitation energy transfer, as specific bioprobes for fluorescent imaging, or as efficient chiroptical dyes exhibiting visible circular dichroism and circularly polarized luminescence. Noticeably, this approach has led to the synthesis of the first CPL-bright chiral-at-boron BODIPYs, a significant novelty in BODIPY chemistry and CPL emitters