Person: Gómez-Lus Centelles, María Luisa
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First Name
María Luisa
Last Name
Gómez-Lus Centelles
Affiliation
Universidad Complutense de Madrid
Faculty / Institute
Medicina
Department
Medicina
Area
Microbiología
Identifiers
36 results
Search Results
Now showing 1 - 10 of 36
Item Is there a pharmacodynamic need for the use of continuous versus intermittent infusion with ceftazidime against Pseudomonas aeruginosa? An in vitro pharmacodynamic model(Journal of Antimicrobial Chemotherapy, 2005) Alou Cervera, Luis; Aguilar, Lorenzo; Sevillano Fernández, David; Giménez, María José; Echeverría, Olatz; Gómez-Lus Centelles, María Luisa; Prieto Prieto, JoséObjectives: In order to explore the pharmacodynamic need for continuous versus intermittent (three times a day) administration of ceftazidime in critically ill patients, a pharmacokinetic computerized device was used to simulate concentrations of ceftazidime in human serum after 6 g/day. Methods: Efficacy was measured as the capability of simulated concentrations over time to reduce initial inoculum against four strains of Pseudomonas aeruginosa. MICs of the strains matched NCCLS breakpoints: one susceptible strain (MIC = 8 mg/L), two intermediate strains (MIC = 16 mg/L) and one resistant strain (MIC = 32 mg/L). C(max) was 119.97+/-2.53 mg/L for intermittent bolus and C(ss) (steady-state concentration) was 40.38+/-0.16 mg/L for continuous infusion. AUC(0-24) was similar for both regimens (approximately 950 mg x h/L). Inhibitory quotients were three times higher for the intermittent administration whereas t > MIC was higher for continuous infusion (100%) versus intermittent administration (99.8%, 69% and 47.6% for the susceptible, intermediate and resistant strains, respectively). Results: Against the susceptible and intermediate strains, no differences were found between both regimens with > or = 3 log10 reduction from 8 to 24 h. Against the resistant strain, only the continuous infusion achieved this bactericidal activity in the same time period, minimizing the differences between resistant and susceptible strains. Significantly higher initial inoculum reduction at 32 h was obtained for the continuous versus the intermittent administration (83.35% versus 38.40%, respectively). Conclusions: These results stress the importance of optimizing t >MIC, even at peri-MIC concentrations, of ceftazidime against resistant strains. Local prevalence of resistance justifies, on a pharmacodynamic basis, electing for continuous infusion versus intermittent administration.- Project number: 243
Item Proyecto EVALUCA para el Fomento de la evaluacion de los sistemas de Calidad Universitarios(2018) Collado Yurrita, Luis Rodolfo; Ciudad Cabañas, María José; Benito León, Julián; Hernández Gallego, Jesús; Gómez-Lus Centelles, María Luisa; Alou Cervera, Luis; Sevillano Fernández, David; Cuadrado Cenzual, María Ángeles; Roiz Sastron, María del Carmen; Verdejo Bravo, Carlos; Madrigal Martínez-Pereda, Cristina María; O´Connor de la Oliva, Ana; Llama Palacios, María Arantxazu; Sánchez Beltrán, María Del Carmen; Menchén Viso, Luis Alberto; Baca González, Laura; Serrano Sánchez, Victor; Vaello Checa, Iris; Marín Viecho, Julia; Córdoba Manzanares, María de los Ángeles; Conty de la Campa, Raquel; Ribeiro Vidal, Honorato José; San Mauro Martín, IsmaelEl Proyecto Evaluca (Evaluación de la Calidad) tiene como objetivo el desarrollo de herramientas que contribuyan al Fomento de la participacion de la Comunidad Universitaria (PDI, PAS, Estudiantes y Egresados) en la evaluación de la calidad. Item Evolving architectural patterns in microbial colonies development(Microscopy Research and Technique, 2010) Gómez‐Aguado, Fernando; Alou Cervera, Luis; Corcuera, María Teresa; Sevillano Fernández, David; Alonso, María José; Gómez-Lus Centelles, María Luisa; Prieto Prieto, JoséAbstract Semithin sections of colonies of three ATCC strains (Staphylococcus aureus, Escherichia coli, and Candida albicans) showed that their internal structure had specific patterns that evolved over the time. These patterns generally were defined by the presence of different layers composed of microorganisms with variable population densities and dead cells. The observed structures in this study could be explained as a particular form of biofilm with an air-semisolid interface.Item Estudiando la microbiota a través de la prevalencia de portadores nasales de Staphylococcus aureus en los estudiantes de Microbiología del Grado de Podología(European Journal of Podiatry, 2020) Gómez-Lus Centelles, María Luisa; Ciudad Cabañas, María José; Collado Yurrita, Luis Rodolfo; Sevillano Fernández, David; González Hidalgo, Natalia; Alou Cervera, LuisObjetivos. Se ha planteado como objetivo la mejora de la calidad de la docencia de la Microbiología mediante la actualización de la metodología docente, introduciendo como actividad el aprendizaje activo basado en la detección de portadores nasales de Staphylococcus aureus para conseguir mejorar las competencias que deberán adquirir los estudiantes como parte de su formación integral. Material y métodos. En este estudio han participado 56 y 72 alumnos (128 alumnos en total) del Grado de Podología de la Universidad Complutense de Madrid de los cursos 2018-2019 y 2019-2020, respectivamente. Los alumnos asistieron al laboratorio de Microbiología y completaron el estudio. Resultados. Un total de 29 alumnos (22,7%) fueron portadores nasales de S. aureus, uno de los cuales fue portador de S. aureus resistente a meticilina (SARM). La valoración de la actividad por parte de los alumnos fue muy positiva y se produjo una mejora general en la adquisición de conocimientos. Conclusión. El trabajo en el laboratorio de Microbiología unido a la actualización en la metodología docente puede mejorar el rendimiento académico de los estudiantes de Microbiología al relacionarlo con su futura práctica profesional y el diagnóstico microbiológico.Item Evaluation of two in vitro pharmacodynamic simulation models: microfiltration versus centrifugation–filtration(International Journal of Antimicrobial Agents, 2001) Alou Cervera, Luis; Sevillano Fernández, David; Bugella, Javier H.; Fuentes, Fernando; Gómez-Lus Centelles, María Luisa; Prieto Prieto, JoséPharmacodynamic in vitro models that simulate serum antimicrobial concentrations provide more information about the activity of an antibiotic than MICs or traditional time-kill methods. The aim of this study was to compare two pharmacodynamic simulation models using ATCC strains of five different species and five antibiotics. In the first model (Centriprep-10 system), a filtration-centrifugation process was used to eliminate the antibiotic; in the second model (microfiltration system) no centrifugation was necessary. The antibiotic concentrations tested were similar to those in serum after normal doses of cefuroxime, clarithromycin, ciprofloxacin, gentamicin and cefotaxime. No significant differences were observed in the killing rates between the models except in the case of Haemophilus influenzae and cefotaxime. The new microfiltration model had the following advantages: lack of the carry-over effect, the absence of centrifugation that could damage bacteria and the possibility of increasing the number of incubation periods to give a better fit of the kinetic profile of man.Item Endogenous cannabinoid system regulates intestinal barrier function in vivo through cannabinoid type 1 receptor activation(American Journal of Physiology Gastrointestinal and Liver Physiology, 2012) Zoppi, Silvia; Muñoz Madrigal, José Luis; Pérez Nievas, Beatriz G.; Marín Jiménez, Ignacio; Caso Fernández, Javier Rubén; Alou Cervera, Luis; García Bueno, Borja; Colón Rodríguez, Arturo Luis; Manzanares, Jorge; Gómez-Lus Centelles, María Luisa; Menchén Viso, Luis Alberto; Leza Cerro, Juan CarlosAbstract The deleterious effects of stress on the gastrointestinal tract seem to be mainly mediated by the induction of intestinal barrier dysfunction and subsequent subtle mucosal inflammation. Cannabinoid 1 receptor (CB1R) is expressed in the mammalian gut under physiological circumstances. The aim of this investigation is to study the possible role of CB1R in the maintenance of mucosal homeostasis after stress exposure. CB1R knockout mice (CB1R−/−) and their wild-type (WT) counterparts were exposed to immobilization and acoustic (IA) stress for 2 h per day during 4 consecutive days. Colonic protein expression of the inducible forms of the nitric oxide synthase and cyclooxygenase (NOS2 and COX2), IgA production, permeability to 51Cr-EDTA, and bacterial translocation to mesenteric lymph nodes were evaluated. Stress exposure induced greater expression of proinflammatory enzymes NOS2 and COX2 in colonic mucosa of CB1R−/− mice when compared with WT animals. These changes were related with a greater degree of colonic barrier dysfunction in CB1R−/− animals determined by 1) a significantly lower IgA secretion, 2) higher paracellular permeability to 51Cr-EDTA, and 3) higher bacterial translocation, both under basal conditions and after IA stress exposure. Pharmacological antagonism with rimonabant reproduced stress-induced increase of proinflammatory enzymes in the colon described in CB1R−/− mice. In conclusion, CB1R exerts a protective role in the colon in vivo through the regulation of intestinal secretion of IgA and paracellular permeability. Pharmacological modulation of cannabinoid system within the gastrointestinal tract might be therapeutically useful in conditions on which intestinal inflammation and barrier dysfunction takes place after exposure to stress. besides its essential digestive function, the gastrointestinal tract represents the main interplay between the host and the environment, exerting an effective but also selective barrier function between the gastrointestinal mucosal immune system and the virtually infinite microbial and alimentary antigens on the mucosal surface. Intestinal epithelial cells constitute the main element of this barrier and exert pivotal roles both in the generation of tolerance toward alimentary antigens and commensal microbiota, and in the activation and orchestration of effective innate and adaptive immune responses (7, 16, 46, 47). However, intestinal barrier is a dynamic structure constituted not only by cellular components but also by an array of noncellular elements such as mucin, antimicrobial peptides, secretory immunoglobulin A (IgA) as well as apical tight junctions between adjacent epithelial cells. Tight junctions are dynamic molecular structures that constitute the rate-limiting seal of the intestinal epithelial barrier paracellular pathway (30). A huge number of proteins take part in the structure of the tight junctions, including zonula occludens (ZO) family proteins, occludin, and the numerous proteins of the claudin family (53); furthermore, the junctional complex is closely related to a ring of actin microfilaments, contraction of which seems to directly regulate paracellular permeability. Inflammatory cytokines such as interferon-γ and tumor necrosis factor-α are capable of regulating tight junction barrier function (31). Intestinal barrier dysfunction leads to the translocation to the lamina propria, lymphatic vessels and portal circulation of luminal bacteria capable of triggering and perpetuating local, and even systemic inflammation. It occurs, for example, in acute pancreatitis and advanced liver cirrhosis; moreover, an adequate transcellular absorption process depends on the presence of an intact tight junction barrier to maintain transepithelial concentration gradients. Indeed, increased intestinal permeability directly related to tight junction dysfunction is a characteristic feature of ulcerative colitis, Crohn's disease, celiac disease, and food allergies (2, 12). Also, intestinal tight junction disruption has been shown in experimental models of stress, assuming their direct responsibility on the increased intestinal permeability that characterizes acute stress in laboratory animals (11, 33, 37). In this sense, exposure to physical and psychological stress triggers and/or modifies the clinical course of a variety of gastrointestinal disorders such as irritable bowel syndrome (IBS) and inflammatory bowel diseases (IBD) (6, 49). Growing evidence from experimental studies supports the ability of psychosocial stress to induce biochemical and histological inflammatory changes in the intestinal mucosa. Indeed, animal stress models represent an excellent tool to assess intestinal barrier physiology and pathophysiology. A common finding observed in several models of stress-induced intestinal inflammation is an increased expression and activity of the inducible isoforms of nitric oxide synthase (NOS2) and cyclooxygenase (COX2) in intestinal tissue homogenates (9). The resulting high concentrations of NO and other reactive oxygen and nitrogen species produced by NOS2 as well as COX2-derived prostaglandin E2 have been involved in barrier dysfunction (3) and water/chloride secretion (48), respectively, associated with intestinal inflammatory conditions. A wide variety of results suggest that such intestinal inflammatory response triggered by psychological and physical stress could be mediated, at least in part, by the induction of intestinal barrier dysfunction resulting in bacterial translocation and enhanced uptake of luminal antigens (9). The endogenous cannabinoid system regulates many different functions in the gastrointestinal system of vertebrates. The two types of cannabinoid receptors (CBR) that have been discovered and cloned, CB1R and CB2R (20), are differentially expressed in the human colon; whereas CB1R is expressed in intestinal epithelial cells, smooth muscle, myenteric plexus, and lamina propria plasma cells, CB2R is mainly expressed under physiological circumstances in plasma cells and macrophages (51) but has also been recently found in myenteric and submucosal neurons of rodent (13) and human bowel samples (52). The effects of CBR activation and the physiological roles for endocannabinoids in the gastrointestinal tract have been extensively reviewed (23); briefly, CB1R activation acts mostly via brain-gut axis to reduce gastrointestinal motility, diarrhea, pain or hyperalgesia, transient lower esophageal sphincter relaxations, emesis, and gastric acid secretion, as well as to promote eating; CB2R activation acts mostly via immune cells to reduce inflammation (39) through, at least, an inhibitory effect on interleukin-8 release in human colonic epithelial cells (21). A role for gastrointestinal carcinogenesis has also been suggested, as downregulation of CB1R and upregulation of CB2R have been observed in intestinal samples of patients with colon cancer (22). However, the role of the endocannabinoid system, and in particular CB1R, in intestinal barrier function and mucosal homeostasis is still largely unknown; however, although the exact mechanisms are poorly understood, some findings support the notion of an endogenous anti-inflammatory activity of CB1R because mice lacking CB1R show enhanced colitis compared with their wild-type (WT) littermates (32, 42). Consistent with this observation, administration of CBR agonists (26) or targeting endocannabinoid degradation (43) has been shown to protect against various forms of experimental colitis in animal models. Nevertheless, the role of the endocannabinoid system in intestinal barrier function has not been previously explored. Therefore, in the present study, we aim to investigate whether CB1R modulates intestinal barrier function in mice exposed to immobilization and acoustic (IA) stress; for this purpose we took advantage of the use of genetically modified mice lacking CB1R as well as pharmacological manipulation of CB1R. We report herein that stress-induced changes were related with a greater colonic barrier permeability and inflammation, lower IgA secretion, and higher bacterial translocation when CB1R is absent.- Project number: 204
Item Curso de formación de profesorado en estructura departamental(2019) Collado Yurrita, Luis Rodolfo; Ciudad Cabañas, María José; Hernández Gallego, Jesús; Gómez-Lus Centelles, María Luisa; Tejedor Jorge, Alberto; Madrigal Martínez-Pereda, Cristina María; Alou Cervera, Luis; Sevillano Fernández, David; Benito León, Julián; Cuenca Caraballo, Maigualida; García Chacón, Marta; O´Connor de la Oliva, Anna; Marín Cuenda, María José; Roiz Sastrón, María del Carmen; San Mauro Martín, Ismael; Marín Viecho, Julia; Muñoz Lucas, María Angeles; Nieto Barbero, María Asunción; Callol Sánchez, Luis Miguel; Sanz Esporrín, Javier; García Torrent, María Jesús; Cuadrado Cenzual, María ÁngelesEl Proyecto propuesto es un Programa Formacion del Profesorado en Dirección y Gestión de Departamentos Universitarios dirigido al PDI, con apoyo del PAS, con la finalidad de obtener un cierto grado de profesionalización en Dirección Departamental. Item Pharmacodynamics of Simulated Total Versus Free-Drug Serum Concentrations of a Low Versus a High Protein Bound Third-Generation Oral Cephalosporin (Cefpodoxime Versus Cefditoren) Against Streptococcus pneumoniae(Journal of Chemotherapy, 2007) Echeverría, Olatz; Alou Cervera, Luis; Sevillano Fernández, David; González Hidalgo, Natalia; Gómez-Lus Centelles, María Luisa; Aguilar, Lorenzo; Prieto Prieto, JoséPharmacodynamic parameters and bactericidal activity against Streptococcus pneumoniae were investigated by simulating total and free serum concentrations of cefpodoxime versus cefditoren. Total drug T>MIC against the penicillin-intermediate (PISP) and resistant (PRSP) strains were 70.6% and 42.9% for cefpodoxime, and 89.6% and 62.5% for cefditoren, respectively. Comparing activity of free versus total cefpodoxime, there were reductions of 8.5% and 19.1% in T>MIC, related to bactericidal activity reductions from approximately 4.5 to 3 log10, and from 3 to 2.5 log10 against PISP and PRSP, respectively, at 10-12h. For cefditoren, reductions of 45.4% and 100% in T>MIC, were related to bactericidal activity reductions from approximately 5.5 to 2-2.5 log10 and from approximately 2.5 to 1.5 log10 against PISP and PRSP, respectively, at 10-12h. Higher differences in activity were found against the less resistant strains when comparing total versus free-drug profile.Item Toll-like 4 receptor inhibitor TAK-242 decreases neuroinflammation in rat brain frontal cortex after stress(Journal of Neuroinflammation, 2014) Gárate, Iciar; García Bueno, Borja; Muñoz Madrigal, José Luis; Caso Fernández, Javier Rubén; Alou Cervera, Luis; Gómez-Lus Centelles, María Luisa; Leza Cerro, Juan CarlosBackground The innate immune response is the first line of defence against invading microorganisms and it is also activated in different neurologic/neurodegenerative pathological scenarios. As a result, the family of the innate immune toll-like receptors (TLRs) and, in particular, the genetic/pharmacological manipulation of the TLR-4 signalling pathway emerges as a potential therapeutic strategy. Growing evidence relates stress exposure with altered immune responses, but the precise role of TLR-4 remains partly unknown. Methods The present study aimed to elucidate whether the elements of the TLR-4 signalling pathway are activated after acute stress exposure in rat brain frontal cortex and its role in the regulation of the stress-induced neuroinflammatory response, by means of its pharmacological modulation with the intravenous administration of the TLR-4 specific inhibitor TAK-242. Considering that TLR-4 responds predominantly to lipopolysaccharide from gram-negative bacteria, we checked whether increased intestinal permeability and a resultant bacterial translocation is a potential regulatory mechanism of stress-induced TLR-4 activation. Results Acute restraint stress exposure upregulates TLR-4 expression both at the mRNA and protein level. Stress-induced TLR-4 upregulation is prevented by the protocol of antibiotic intestinal decontamination made to reduce indigenous gastrointestinal microflora, suggesting a role for bacterial translocation on TLR-4 signalling pathway activation. TAK-242 pre-stress administration prevents the accumulation of potentially deleterious inflammatory and oxidative/nitrosative mediators in the brain frontal cortex of rats. Conclusions The use of TAK-242 or other TLR-4 signalling pathway inhibitory compounds could be considered as a potential therapeutic adjuvant strategy to constrain the inflammatory process taking place after stress exposure and in stress-related neuropsychiatric diseases.Item Perspectivas Educativas en Microbiología: Análisis del Impacto de las Prácticas Virtuales vs las Prácticas Presenciales en Alumnos del Grado de Podología(Revista Española de Educación Médica, 2024) González Hidalgo, Natalia; Gómez-Lus Centelles, María Luisa; Sevillano Fernández, David; Alou Cervera, LuisCon el objetivo de integrar de manera efectiva los entornos virtuales en las prácticas de Microbiología, se ha analizado la eficacia de las prácticas en formato virtual en comparación con el presencial en la asignatura de Microbiología del Grado de Podología. Tras elegir el formato práctico presencial o virtual, los alumnos realizaron voluntariamente cuestionarios de conocimientos previos y posteriores a las prácticas, con el fin de evaluar su rendimiento. Asimismo, se evaluó el compromiso y satisfacción de los estudiantes. La mayoría de los alumnos eligieron realizar las prácticas en formato presencial, mostrando un mayor compromiso y participación en los cuestionarios. Ambos grupos experimentaron una mejora en el rendimiento, sin diferencias significativas entre formatos. Los estudiantes que participaron en las prácticas expresaron un alto nivel de satisfacción en ambos formatos. Las prácticas virtuales suponen una alternativa adecuada a las prácticas presenciales en la enseñanza de Microbiología.