Electrochemical biosensor based on ionic liquid polymeric microparticles. An analytical platform for catechol

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dc.contributor.authorSánchez-Paniagua López, Marta
dc.contributor.authorLópez Ruiz, María Beatriz
dc.date.accessioned2024-02-07T16:43:39Z
dc.date.available2024-02-07T16:43:39Z
dc.date.issued2018-06-10
dc.description.abstractA catechol amperometric biosensor based on tyrosinase has been successfully developed. Tyrosinase was immobilized into microparticles prepared by polymerization of the ionic liquid 1-vinyl-3-ethyl-imidazolium bromide (ViEtIm+Br−). Different kinds of microparticles with entrapped tyrosinase based on ionic liquid were obtained from the latter, by a simple anion-exchange reaction. The synthesis of the microparticles was optimized. The analytical method based on the proposed device was also optimized in terms of pH and temperature. Optimal values were 6.5 and 25 °C, respectively. The characterization of both, microparticles and analytical device, was performed by cyclic voltammetry and electrochemical impedance spectroscopy. A linear relationship between the current signal and the catechol concentration was obtained in the range from 3.9·10−5 to 2.5·10−4 M, with a sensitivity of 17.95 mA M−1 cm−2 and a detection limit of 20 μM. The tyrosinase activity of lyophilized poly(ViEtIm+Br−) microparticles was unchanged for at least 9 months after their synthesis. The biosensor maintains 95% of the initial response 21 days after its first use. The biosensor has been applied for detecting phenol compounds in wastewater samples. The recoveries were found in the range of 91–96%.eng
dc.description.departmentDepto. de Química Analítica
dc.description.facultyFac. de Farmacia
dc.description.refereedTRUE
dc.description.statuspub
dc.identifier.citationSánchez-Paniagua López M, López-Ruiz B. Electrochemical biosensor based on ionic liquid polymeric microparticles. An analytical platform for catechol. Microchemical Journal 2018;138:173–9. https://doi.org/10.1016/j.microc.2018.01.011.
dc.identifier.doi10.1016/j.microc.2018.01.011
dc.identifier.essn1095-9149
dc.identifier.issn0026-265X
dc.identifier.officialurlhttps://doi.org/10.1016/j.microc.2018.01.011
dc.identifier.urihttps://hdl.handle.net/20.500.14352/100088
dc.language.isoeng
dc.rights.accessRightsrestricted access
dc.subject.keywordPolymeric microparticles
dc.subject.keywordIonic liquid
dc.subject.keywordElectrochemical biosensor
dc.subject.keywordTyrosinase
dc.subject.keywordCatechol
dc.subject.keywordWaste water
dc.subject.ucmQuímica analítica (Química)
dc.subject.unesco2301 Química Analítica
dc.titleElectrochemical biosensor based on ionic liquid polymeric microparticles. An analytical platform for catecholen
dc.typejournal article
dspace.entity.typePublication
relation.isAuthorOfPublication966f7c2f-7001-4961-8820-69b6b96765b5
relation.isAuthorOfPublication82f03299-d29c-41d8-a9ff-c450783e993d
relation.isAuthorOfPublication.latestForDiscovery966f7c2f-7001-4961-8820-69b6b96765b5
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