Transgene expression in mice of the Opa1 mitochondrial transmembrane protein through bicontinuous cubic lipoplexes containing gemini imidazolium surfactants

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dc.contributor.authorMuñoz Úbeda, Mónica
dc.contributor.authorSemenzato, Martina
dc.contributor.authorFranco-Romero, Anais
dc.contributor.authorJunquera González, María Elena
dc.contributor.authorAicart Sospedra, Emilio
dc.contributor.authorScorrano, Luca
dc.contributor.authorLópez Montero, Iván
dc.date.accessioned2024-01-11T15:36:14Z
dc.date.available2024-01-11T15:36:14Z
dc.date.issued2021
dc.description.abstractLipoplexes are non-viral vectors based on cationic lipids used to deliver DNA into cells, also known as lipofection. The positively charge of the hydrophilic head-group provides the cationic lipids the ability to condensate the negatively charged DNA into structured complexes. The polar head can carry a large variety of chemical groups including amines as well as guanidino or imidazole groups. In particular, gemini cationic lipids consist of two positive polar heads linked by a spacer with different length. As for the hydrophobic aliphatic chains, they can be unsaturated or saturated and are connected to the polar head-groups. Many other chemical components can be included in the formulation of lipoplexes to improve their transfection efficiency, which often relies on their structural features. Varying these components can drastically change the arrangement of DNA molecules within the lamellar, hexagonal or cubic phases that are provided by the lipid matrix. Lipofection is widely used to deliver genetic material in cell culture experiments but the simpler formulations exhibit major drawbacks related to low transfection, low specificity, low circulation half-life and toxicity when scaled up to in vivo experiments.
dc.description.departmentDepto. de Química Física
dc.description.facultyFac. de Ciencias Químicas
dc.description.refereedTRUE
dc.description.sponsorshipComunidad de Madrid
dc.description.sponsorshipMinisterio de Ciencia e Innovación (España)
dc.description.sponsorshipUniversidad Complutense de Madrid
dc.description.statuspub
dc.identifier.citationMuñoz-Úbeda, M., Semenzato, M., Franco-Romero, A. et al. Transgene expression in mice of the Opa1 mitochondrial transmembrane protein through bicontinuous cubic lipoplexes containing gemini imidazolium surfactants. J Nanobiotechnol 19, 425 (2021). https://doi.org/10.1186/s12951-021-01167-x
dc.identifier.doi10.1186/s12951-021-01167-x
dc.identifier.issn1477-3155
dc.identifier.officialurlhttps://doi.org/10.1186/s12951-021-01167-x
dc.identifier.urihttps://hdl.handle.net/20.500.14352/92587
dc.issue.number425
dc.journal.titleJournal of Nanobiotechnology
dc.language.isoeng
dc.publisherSpringer Nature
dc.rights.accessRightsopen access
dc.subject.cdu544
dc.subject.ucmCiencias
dc.subject.unesco23 Química
dc.titleTransgene expression in mice of the Opa1 mitochondrial transmembrane protein through bicontinuous cubic lipoplexes containing gemini imidazolium surfactants
dc.typejournal article
dc.type.hasVersionVoR
dc.volume.number19
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery270b8d45-a3ee-4257-8b98-17d43064b34e
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