The GDP-Bound State of Mitochondrial Mfn1 Induces Membrane Adhesion of Apposing Lipid Vesicles through a Cooperative Binding Mechanism

Simple item page
dc.contributor.authorTolosa Díaz, Andrés
dc.contributor.authorAlmendro Vedia, Víctor Galileo
dc.contributor.authorNatale, Paolo
dc.contributor.authorLópez Montero, Iván
dc.date.accessioned2023-06-17T09:12:00Z
dc.date.available2023-06-17T09:12:00Z
dc.date.issued2020-07-21
dc.description.abstractMitochondria are double-membrane organelles that continuously undergo fission and fusion. Outer mitochondrial membrane fusion is mediated by the membrane proteins mitofusin 1 (Mfn1) and mitofusin 2 (Mfn2), carrying a GTP hydrolyzing domain (GTPase) and two coiled-coil repeats. The detailed mechanism on how the GTP hydrolysis allows Mfns to approach adjacent membranes into proximity and promote their fusion is currently under debate. Using model membranes built up as giant unilamellar vesicles (GUVs), we show here that Mfn1 promotes membrane adhesion of apposing lipid vesicles. The adhesion forces were sustained by the GDP-bound state of Mfn1 after GTP hydrolysis. In contrast, the incubation with the GDP:AlF− 4 , which mimics the GTP transition state, did not induce membrane adhesion. Due to the flexible nature of lipid membranes, the adhesion strength depended on the surface concentration of Mfn1 through a cooperative binding mechanism. We discuss a possible scenario for the outer mitochondrial membrane fusion based on the modulated action of Mfn1.
dc.description.departmentDepto. de Química Física
dc.description.departmentSección Deptal. de Farmacia Galénica y Tecnología Alimentaria (Veterinaria)
dc.description.facultyFac. de Ciencias Químicas
dc.description.facultyFac. de Veterinaria
dc.description.refereedTRUE
dc.description.sponsorshipUnión Europea. FP7
dc.description.statuspub
dc.eprint.idhttps://eprints.ucm.es/id/eprint/67538
dc.identifier.doi10.3390/biom10071085
dc.identifier.issn2218-273X
dc.identifier.officialurlhttps://doi.org/10.3390/biom10071085
dc.identifier.relatedurlhttps://www.mdpi.com/2218-273X/10/7/1085
dc.identifier.urihttps://hdl.handle.net/20.500.14352/8380
dc.issue.number7
dc.journal.titleBiomolecules
dc.language.isoeng
dc.page.initial1085
dc.publisherMDPI
dc.relation.projectIDMITOCHON (338133)
dc.rightsAtribución 3.0 España
dc.rights.accessRightsopen access
dc.rights.urihttps://creativecommons.org/licenses/by/3.0/es/
dc.subject.keywordmitochondria
dc.subject.keywordmembrane fusion
dc.subject.keywordGTPase
dc.subject.keywordmitofusin 1
dc.subject.keywordgiant vesicles
dc.subject.ucmBiología celular (Biología)
dc.subject.ucmFísica
dc.subject.unesco2407 Biología Celular
dc.titleThe GDP-Bound State of Mitochondrial Mfn1 Induces Membrane Adhesion of Apposing Lipid Vesicles through a Cooperative Binding Mechanism
dc.typejournal article
dc.volume.number10
dspace.entity.typePublication
relation.isAuthorOfPublicationcac874a1-a328-4d98-a6a4-7a594f6573c7
relation.isAuthorOfPublication04e36158-d33c-41b2-b16c-efafa44a8bca
relation.isAuthorOfPublicationf695bacc-278b-4155-93dc-eaa4b0ec28fe
relation.isAuthorOfPublication.latestForDiscovery04e36158-d33c-41b2-b16c-efafa44a8bca
Download
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
biomolecules-10-01085-v2.pdf
Size:
1.22 MB
Format:
Adobe Portable Document Format
downoload Download
Collections
Artículos