Citation: Palá-Paúl, J.; Abad-Calderón, R.; Pérez-Alonso, M.J.; Brophy, J.J.; Soria, A.C. The Essential Oil Composition of Eryngium galioides Lam.—An Endemic Species of the Iberian Peninsula. Separations 2024, 11, 172. https:// doi.org/10.3390/separations11060172 Academic Editors: Paraskevas D. Tzanavaras and Gavino Sanna Received: 19 April 2024 Revised: 17 May 2024 Accepted: 26 May 2024 Published: 1 June 2024 Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// creativecommons.org/licenses/by/ 4.0/). separations Article The Essential Oil Composition of Eryngium galioides Lam.— An Endemic Species of the Iberian Peninsula Jesús Palá-Paúl 1,* , Rubén Abad-Calderón 2, María José Pérez-Alonso 1, Joseph J. Brophy 3 and Ana C. Soria 4 1 Department of Biodiversity, Ecology and Evolution, Faculty of Biological Sciences, Complutense University of Madrid, 28040 Madrid, Spain; mjpa32@ucm.es 2 Department of Chemistry in Pharmaceutical Sciences, Teaching Unit of Soil Science, Faculty of Pharmacy, Complutense University of Madrid, 28040 Madrid, Spain; rubabad@ucm.es 3 School of Chemistry, The University of New South Wales, Sydney, NSW 2052, Australia; j.brophy@unsw.edu.au 4 Instituto de Química Orgánica General (IQOG-CSIC), Juan de la Cierva 3, 28006 Madrid, Spain; acsoria@iqog.csic.es * Correspondence: quibey@bio.ucm.es Abstract: The Eryngium L. genus belongs to the Apiaceae family and, with about 250 species, has a cosmopolitan distribution. Only fourteen of the twenty-six species described in Flora Europaea grow in the Iberian Peninsula. One of these is Eryngium galioides Lam., a small annual plant (2–30 cm) that grows in open dry places in the mid-west of the Iberian Peninsula. For this study, the whole plant (aerial parts and roots) of this species was gathered in Guadalajara (Spain). The essential oil of this population was extracted by hydro-distillation and analyzed by gas chromatography (GC) and gas chromatography coupled to mass spectrometry (GC-MS). It is worth noting that this species gave rise to a relatively high essential oil yield (0.48%) in comparison with other species of this genus. E. galioides oil consisted of a complex mixture of more than 70 compounds. The main constituents of this oil were identified as valencene (49.7%) and a phyllocladene isomer (23.7%), both representing more than the 70% of the total oil. Other representative compounds of this oil were found to be β-chamigrene (6.0%), γ-muurolene (3.4%), (E)-caryophyllene (3.0%) and β-elemene (1.6%). As far as we know, this is the first report about the chemical composition of E. galioides essential oils. With this work, we contribute to the knowledge of this genus and provide a chemical and botanical basis to promote the in vitro cultivation of E. galioides as a source of essential oils rich in bio-actives for application in different fields. Keywords: Eryngium galioides; Eryngium; Apiaceae; essential oil; chemical composition; valencene and phyllocladene isomer 1. Introduction The Apiaceae is known worldwide as the parsley family, comprising about 434 genera and nearly 3.780 species of plants distributed throughout a wide variety of habitats mainly in the north temperate regions of the world [1]. Many of those species are economically important as leaf, root vegetables, herbs, spices and garden ornamentals and widely cul- tivated, including such species as Apium graveolens L. (Celery), Daucus carota L. (Carrot), Foeniculum vulgare Mill. (Fennel), Anethum graveolens L. (Dill), Petroselinum crispum (Mill.) Nyman ex A.W. Hill (Parsley), Coriandrum sativum L. (Coriander), Carum carvi L. (Caraway), Cuminum cyminum L. (Cumin) and Pimpinella anisum L. (Anise). Moreover, most of the species analyzed to date have different biological activities (antibacterial, antifungal, herbi- cidal, insecticidal or repellent) that support their culinary use not only as a flavoring [2]. However, other species are source of other type of bio-active compounds such Conium maculatum L. (Hemlock) that has been used as poison [3,4]. Separations 2024, 11, 172. https://doi.org/10.3390/separations11060172 https://www.mdpi.com/journal/separations https://doi.org/10.3390/separations11060172 https://doi.org/10.3390/separations11060172 https://creativecommons.org/ https://creativecommons.org/licenses/by/4.0/ https://creativecommons.org/licenses/by/4.0/ https://www.mdpi.com/journal/separations https://www.mdpi.com https://orcid.org/0000-0002-6415-2295 https://orcid.org/0000-0003-1408-9782 https://doi.org/10.3390/separations11060172 https://www.mdpi.com/journal/separations https://www.mdpi.com/article/10.3390/separations11060172?type=check_update&version=1 Separations 2024, 11, 172 2 of 15 The genus Eryngium L., which belongs to this family and comprises about 250 species, has a cosmopolitan distribution. It exhibits great morphological and habitat diversity and includes annual and perennial herbs with hairless and spiny leaves that present dome- shaped umbels of steely blue or white flowers with whorls of spiny basal bracts. Only fourteen of the twenty-six species described in Flora Europaea grow wildly in the Iberian Peninsula [5]. Eryngium galiodes Lam. is a small annual plant (2–30 cm), rarely biennial, that is spinescent in the inflorescences. The roots are more or less fasciculated, dark brown. Stems, 0.1–0.25 cm in diameter at the base, range from almost undivided to profusely branched, often from the base itself, and are straw colored. Basal leaves (2–8 × 0.2–0.12 cm), are sparse, linear-lanceolate or oblanceolate. The cauline leaves are scattered and scarcely branched. Inflorescences on hemispherical capitula, barely visible from the involvement, are sessile or almost so. It produces small fruits in mericarps (1.5 mm), mostly naked, with elongated scales on top [6]. It grows in open dry places in the mid-west of the Iberian Peninsula according to the vouchers that have been revised from different Portuguese and Spanish herbaria (Figure 1). According to the European Nature Information System (EUNIS) and following the International Union for de Conservation of the Nature (Unión Internacional para la Conservación de la Naturaleza: UICN) status, E. galioides is considered endangered [7]. Separations 2024, 11, x FOR PEER REVIEW 2 of 15 The genus Eryngium L., which belongs to this family and comprises about 250 species, has a cosmopolitan distribution. It exhibits great morphological and habitat diversity and includes annual and perennial herbs with hairless and spiny leaves that present dome- shaped umbels of steely blue or white flowers with whorls of spiny basal bracts. Only fourteen of the twenty-six species described in Flora Europaea grow wildly in the Iberian Peninsula [5]. Eryngium galiodes Lam. is a small annual plant (2–30 cm), rarely biennial, that is spi- nescent in the inflorescences. The roots are more or less fasciculated, dark brown. Stems, 0.1–0.25 cm in diameter at the base, range from almost undivided to profusely branched, often from the base itself, and are straw colored. Basal leaves (2–8 × 0.2–0.12 cm), are sparse, linear-lanceolate or oblanceolate. The cauline leaves are scattered and scarcely branched. Inflorescences on hemispherical capitula, barely visible from the involvement, are sessile or almost so. It produces small fruits in mericarps (1.5 mm), mostly naked, with elongated scales on top [6]. It grows in open dry places in the mid-west of the Iberian Peninsula according to the vouchers that have been revised from different Portuguese and Spanish herbaria (Figure 1). According to the European Nature Information System (EUNIS) and following the International Union for de Conservation of the Nature (Unión Internacional para la Conservación de la Naturaleza: UICN) status, E. galioides is consid- ered endangered [7]. Figure 1. Distribution of Eryngium galiodes Lam. in the Iberian Peninsula. A genus like this with a wide distribution has been the object of different studies. Focusing on the last 20 years, we have found 74 papers describing essential oils [8–56], 3 reviews [57–59] and 23 studies of their biological activities [60–81]. Although more than 70 papers have been published related to this genus, only 37 of the approximately 250 described species have been reported. Most of them relative to the chemical composition of the essential oils. The species analyzed to date in alphabetical order are Eryngium al- pinum [8], Eryngium amethystinum [8–11], Eryngium aquifolium [12], Eryngium barrelieri [13], Eryngium billardieri [14,15], Eryngium bornmuelleri [16], Eryngium bourgatii [17], Eryngium bungei [18], Eryngium caeruleum [19,20], Eryngium campestre [9,10,21,22], Eryngium caucasi- cum [23,24], Eryngium corniculatum [25], Eryngium creticum [26,27], Eryngium dilatatum [28], Eryngium duriaei [29,30], Eryngium eriophorum [31], Eryngium expansum [32], Eryngium flo- ribundum [31], Eryngium foetidum [33–38], Eryngium glaciale [39], Eryngium horridum [31], Eryngium maritimum [40–45], Eryngium nudicaule [31], Eryngium palmatum [46], Eryngium palmatum [9,46–49], Eryngium paludosum [48], Eryngium pandanifolium [31,32], Eryngium pa- niculatum [49], Eryngium planum [50], Eryngium pseudothoriifolium [51], Eryngium pyrami- dale [52], Eryngium rostratum [32], Eryngium serbicum [47], Eryngium thorifolium [51], Eryn- gium tricuspidatum [53], Eryngium triquetrum [54,55] and Eryngium vesiculosum [32–56]. In addition to the culinary use of some of these species [74,78], a number of biological activ- ities of any of these activities have been ascribed to them, inlcuding analgesic [52], anti- bacterial [67,69,76], anti-inflammatory [52,72], antimicrobial [62,66,81], antioxidant Figure 1. Distribution of Eryngium galiodes Lam. in the Iberian Peninsula. A genus like this with a wide distribution has been the object of different studies. Focusing on the last 20 years, we have found 74 papers describing essential oils [8–56], 3 reviews [57–59] and 23 studies of their biological activities [60–81]. Although more than 70 papers have been published related to this genus, only 37 of the approximately 250 described species have been reported. Most of them relative to the chemical composi- tion of the essential oils. The species analyzed to date in alphabetical order are Eryngium alpinum [8], Eryngium amethystinum [8–11], Eryngium aquifolium [12], Eryngium barrelieri [13], Eryngium billardieri [14,15], Eryngium bornmuelleri [16], Eryngium bourgatii [17], Eryngium bungei [18], Eryngium caeruleum [19,20], Eryngium campestre [9,10,21,22], Eryngium cauca- sicum [23,24], Eryngium corniculatum [25], Eryngium creticum [26,27], Eryngium dilatatum [28], Eryngium duriaei [29,30], Eryngium eriophorum [31], Eryngium expansum [32], Eryngium flori- bundum [31], Eryngium foetidum [33–38], Eryngium glaciale [39], Eryngium horridum [31], Eryngium maritimum [40–45], Eryngium nudicaule [31], Eryngium palmatum [46], Eryngium palmatum [9,46–49], Eryngium paludosum [48], Eryngium pandanifolium [31,32], Eryngium paniculatum [49], Eryngium planum [50], Eryngium pseudothoriifolium [51], Eryngium pyrami- dale [52], Eryngium rostratum [32], Eryngium serbicum [47], Eryngium thorifolium [51], Eryn- gium tricuspidatum [53], Eryngium triquetrum [54,55] and Eryngium vesiculosum [32–56]. In addition to the culinary use of some of these species [74,78], a number of biological activities of any of these activities have been ascribed to them, inlcuding analgesic [52], antibacte- rial [67,69,76], anti-inflammatory [52,72], antimicrobial [62,66,81], antioxidant [65,67,69,78], antiprotozoal [80], cytotoxic [64,66,69], insecticidal [74], molluscicidal and parasiticidal [68] Separations 2024, 11, 172 3 of 15 activity. Other less common activities include their use as a corrosion inhibitor [75] or preservative [61,70] and their effectiveness in hyperglycemia [63], improving long-term memory [71], and percutaneous absorption [77]. As far as we know, the essential oils of only eight of the fourteen species that grow in the Iberian Peninsula have been studied to date. Table 1 shows the species studied, the part of the plant used, the main compounds identified and the oil yield of each one. According to these results, the essential oils of these species are richer in sesquiterpenes than in other terpenoids. However, this is not a general rule, as the habitat where each one grows and the size (biotype) of the species seem to be more significant. The species growing under hard climatic conditions and at high altitude that are geophytes or hemicryptophytes contain a great amount of diterpenes—compounds making up the predominant fraction of the oil in Eryngium bourgatii [17] and E. glaciale [38]. In fact, altitude has been described to affect the chemical composition of the Iberian endemic species, E. duriaei [28]. The populations that grow below 1700 m showed α-neocallitropsene (28–53%), β-betulenal (8.5–15.8%) and 14-hydroxy-β-caryophyllene (5.8–13.7%) as their main compounds, while caryophyllene oxide (47%) and E-caryophyllene (6%) were identified in the analyzed population over this altitude [28]. On the other hand, species growing close to wet areas or in seasonal lakes while, normally therophytes, such as E. corniculatum, exhibited monoterpenes as the largest fraction of the oils [25]. The chemical composition of other species (e.g., E. campestre) growing under similar climatic conditions seem to be affected by the type of soil of each population [22]. Table 1. Main constituents of the essential oils of the Eryngium species-growing wild in the Iberian Peninsula. Species Main Components Yield (%) Ref. E. aquifolium Cav. inflorescences oil: germacrene D (30.3%) and sesquicineole (26.7%) 0.81 [12]stems and leaves oil: germacrene D (46.0%) and myrcene (13.8%) 0.41 roots oil: phyllocladene isomer (63.6%) 0.18 E. bourgatii Gouan inflorescences oil: phyllocladene (37.6%) and bicyclogermacrene (15.1%) 0.33 [17] stems and leaves oil: phyllocladene (20.4%), γ-muurolene (11.8%) and (E)-caryophyllene (10.1%) 0.11 roots oil: γ-muurolene (15.4%) and phyllocladene (15.0%) 0.20 E. campestre L. inflorescences oil: germacrene D (30.3–40.3%), β-curcumene (0.7–22.2%), myrcene (3.0–21.7%), (E)-β-farnesene (0.1–19.0%). 0.1–0.4 [22] stems and leaves oil: germacrene D (31.1–42.4%), myrcene (0.5–23.15) 0.1–0.2 E. corniculatum Lam. inflorescences oil: 2,4,6-trimethylbenzaldehyde (50.8%), α-pinene (4.0%) 0.82 [25] stems and leaves oil: 2,4,6-trimethylbenzaldehyde (50.0%), 2,4,5-trimethylbenzaldehyde (3.8%) 0.49 roots oil: 2,4,6-trimethylbenzaldehyde (29.8%), phyllocladene isomer (13.0%), (E)-nerolidol (9.4%) 0.22 Separations 2024, 11, 172 4 of 15 Table 1. Cont. Species Main Components Yield (%) Ref. Eryngium dilatatum Lam. inflorescences oil: α-cadinol (3.8%), bicyclogermacrene (3.5%), octanal (3.1%) and spathulenol (2.5%) 0.29 [28] stems and leaves oil: octanal (8.1%), α-cadinol (3.7%), δ-cadinene (3.6%), (E)-caryophyllene (2.6%), bicyclogermacrene (2.5%) and spathulenol (2.4%) 0.33 roots oil: spathulenol (4.6%), α-cadinol (4.4%), khusinol (3.2%), α-muurolol (3.1%) and δ-cadinene (2.6%) 0.14 E. duriaei J. Gay ex Boiss. populations below 1700 m: α-neocallitropsene (28–53%), β-betulenal (8.5–15.8%) and 14-hydroxy-β-caryophyllene (5.8–13.7%) 0.2–0.3 [29] population over 1700 m: caryophyllene oxide (47%) and E-caryophyllene (6%) Eryngium duriaei subsp. juresianum (M. Laínz) M. Laínz aerial parts: α-neocallitropsene (26.0%), isocaryophyllen-14-al (16.2%), 14-hidroxy-β-caryophyllene (13.4%), caryophyllene oxide (7.6%) and (E)-β-caryophyllene (6.3%). 0.15 [30] E. glaciale Boiss. inflorescences oil: phyllocladene isomer (43.5%), (E)-caryophyllene (15.2%) and valencene (11.5%) 0.16 [39]stems and leaves oil: phyllocladene isomer (41.3%) 0.26 roots oil: phyllocladene isomer (49.4%) and linalool (19.1%) 0.30 Two species with wide distribution, E. campestre and E. maritimum, showed similar variations growing out of the Iberian Peninsula. Table 2 compiles their main components and oil yield together with their provenance for these samples. Table 2. Main constituents of the essential oils of the Eryngium species-growing wild out of the Iberian Peninsula. Species Region Main Components Yield (%) Ref. E. campestre L. Italy (Perugia) aerial parts: germacrene D (13.8%), allo-aromadendrene (7.7%), spathulenol (7.0%) and ledol (5.7%) 0.04 [10] Algeria (Tlemcen) aerial parts: germacrene D (15.2%), campestrolide (10.3%), spathulenol (4.8%) and α-cadinol (5.5%) 0.1–0.2 [21] E. maritimum L. Latvia leaves (wild plants): 4βH-muurol-9-en-15-al > germacrene D > spathulenol > 4βH-cadin-9-en-15-ol 0.15–0.54 [40] leaves (cultivated plants): germacrene D (61.13–75.05%) > eudesma-4,7-diene-1β-ol > cumene > α-muurolene 0.15–0.54 Separations 2024, 11, 172 5 of 15 Table 2. Cont. Species Region Main Components Yield (%) Ref. E. maritimum L. Poland (Poznań) leaves: 2,3,4-trimethylbenzaldehyde (11.3%) and germacrene D (10.5%) 0.06 [41] fruits: germacrene D (45.2%) 0.3 roots: hexadecanoic acid (18.5%), menthol (16.8%) and menthone (10.9%) 0.01 Tunisia fruits: germacrene D (13.62–31.71%), 15-hydroxy-α-muurolene (12.04–18.58%) and germacrene B (6.77–15.04%). 0.31–0.93 [42] Corsica and Sardinia aerial parts: germacrene D (13.7–45.9%), 4βH-cadin-9-en-15-al (18.4–27.6%), 4βH-cadin-9-en-15-ol (2.2–14.3%) and 4βH-muurol-9-en-15-al (4.3–9.3%) 0.06–0.13 fresh weight [43] roots: 2,4,5-trimethylbenzaldehyde (39.8%), 2,3,6-trimethylbenzaldehyde (29.0%) and α-muurolene (23.5%) 0.06–0.13 fresh weight Sicily (Palermo) aerial parts: germacrene D (10.4%) and 2,4,5-trimethylbenzaldehyde (8.3%) 0.93 [44] roots: germacrene D (15.9%) and 2,4,5-trimethylbenzaldehyde (6.7%) 0.84 Corsica (Quercionu) aerial parts: isolation of 4βH-muurol-9-en-15-al, 4βH -cadin-9-en-15-al, 4βH-muurol-9-en-15-ol and 4βH-cadin-9-en-15-ol 0.08 [45] The aim of this study was to analyze the essential oils of E. galioides and compare its chemical composition with those of other Iberian species studied to date. This study contributes to the knowledge of the chemical composition of the Eryngium genus species. As far as we know, this is the first report on the volatile components of this species, which is endemic to the Iberian Peninsula. 2. Materials and Methods 2.1. Plant Material Several specimens of Eryngium galioides were gathered at flowering in Puebla de Beleña (30TVL7827) (22-VI-2000), Guadalajara, Castilla-La Mancha (Spain). A voucher specimen (MACB-75554) was lodged at the Herbarium of the Faculty of Biology, Complutense University, Madrid, Spain. 2.2. Isolation of Volatile Oils All the samples of Eryngium galioides, including inflorescences, stems, leaves and roots were air-dried. Because of the small size, the complete plant was subjected to hydro distillation with cohobation for 8 h according to the method recommended in the Spanish Pharmacopoeia [82] to obtain its essential oils. The oil was dried over anhydrous magnesium sulphate and stored at 4 ◦C in the dark. The yield (%) of the essential oil was calculated on a dry weight basis. The yield, on a dry weight basis, of the essential oil analyzed was 0.48%. 2.3. Gas Chromatography (GC) GC analysis was carried out on a Varian 3300 gas chromatograph fitted with a fused silica methyl silicone DB-1 column (50 m × 0.25 mm, 0.25 µm film thickness). The tempera- ture was programmed from 95 to 240 ◦C at 4 ◦C min−1. Injection was performed at 250 ◦C Separations 2024, 11, 172 6 of 15 in the split mode (1:100). Nitrogen was used as the carrier gas (1.5 mL min−1). Detection was carried out using a flame ionization detector (FID) (Palo alto, California, USA)at 300 ◦C. The injection volume for all the samples was 0.1 µL of pure oil. 2.4. Gas Chromatography–Mass Spectrometry (GC-MS) GC-MS analyses were carried out on a 6890 gas chromatograph coupled to an HP 5973 mass selective detector (both from Agilent Technologies, Santa Clara, CA, USA). A fused silica SE-30 capillary column (50 m × 0.22 mm, 0.25 µm film thickness) was used for separation. The column temperature was programmed from 70 to 220 ◦C at 4 ◦C min−1, and Helium at 1 mL min−1 was used as carrier gas. Mass spectra were recorded in the scan mode (35–450 m/z range) at 70 eV. In order to confirm the identification of several compounds, the oil samples were also analyzed on a VG Quattro gas chromatograph-mass spectrometer operating at 70 eV ionization energy. The GC column used was a DB-Wax (60 m × 0.32 mm, ×0.25 µm) programmed from 35 ◦ to 220 ◦C at 3 ◦C min−1, with Helium (1 mL min−1) as carrier gas. 2.5. Qualitative and Quantitative Analyses Most constituents were tentatively identified by GC through a comparison of their retention indices with those of authentic standards available in the authors’ laboratory or retention indices from literature [83–89]. Further identification was achieved by GC- MS: the fragmentation patterns of experimental mass spectra were compared with those stored in the commercial spectrometer data base using the WILEY.L built-in library. Other constituents were either synthesized or identified in oils of known composition. Semiquantitative analysis (%) was carried out directly from peak areas in the GC profile. 3. Results and Discussion The whole plant of Eryngium galioides provided a pale-yellow essential oil (0.48% extraction yield on a dry weight basis). Despite the small size of this species, its yield was higher than the mean of the other Eryngium species growing in the Iberian Peninsula (Table 1). A reason for this could be that the habitat where it grows is not as hard as that of other species such as E. buorgatii and E. glaciale growing in high mountains, but harder than others as E. corniculatum, which lives on the margins of seasonal lakes and, therefore, disposes of water during its flowering and fruiting. In agreement with previous references [10,12,17,21,22,25,28–30,39–45], our results indicate that the species of this genus are perfectly adapted to the environment in which they develop, and that may affect the yield of their essential oils. It would, however, be interesting to analyze the plasticity of these species under controlled conditions to understand the possible effect of climate change on their survival and distribution. Some of these species are catalogued as vulnerable or endangered, so this could help us to understand the possible risk in that scenario. The components identified in the Eryngium galioides essential oil under study, their retention indices, and their percent composition are summarized in Table 3, where they are all arranged by their elution order on the DB-1 column. The retention indices of these compounds on the DB-Wax column are also listed in brackets in that table. As is known, essential oils are mixtures of aromatic and aliphatic compounds in different concentrations, all of which contribute to the perceived aroma of the plant. In our case, a total of 71 compounds were identified, representing the 97.3% of the total oil analyzed. It is worth noting that only two compounds, valencene (49.7%) and a phyllocladene isomer (23.7%), were identified as the main components of this oil, the sum of both comprising more than the 70% of the total oil. The remainder of the components that contribute to the scent of this species did not obtain a percentage over 6.0%. Some of these could be considered representative, including β-chamigrene (6.0%), γ-muurolene (3.4%), (E)-caryophyllene (3.0%), β-elemene (1.6%), nonane (1.4%), δ-cadinene (1.0%) and spathulenol (1.0%). Separations 2024, 11, 172 7 of 15 Table 3. Essential oil composition (in %) from the whole plant (roots + steams + leaves + flowers) of Eryngium galioides Lam. from Spain. Compound I I1it %. IM nonane 869 900 1.4 MS,1 α-pinene 932 (1012) 939 0.2 MS,2 heptanol 955 (1181) 959 t MS,1 sabinene 963 (1113) 969 t MS,2 β-pinene 970 (1097) 974 t MS,2 myrcene 985 (1160) 988 0.3 MS,2 octanal 993 (1286) 988 0.2 MS,1 α-phellandrene 1005 (1157) 1002 t MS,2 o-cimene 1019 (1268) 1022 t MS,2 limonene 1026 (1191) 1024 t MS,2 (Z)-β-ocimene 1031 (1232) 1032 t MS,1 benzene acetaldehyde 1032 1036 t MS,1 (E)-β-ocimene 1041 (1249) 1044 t MS,1 γ-terpinene 1058 (1240) 1054 t MS,2 octanol 1063 (1172) 1063 t MS,2 fenchone 1074 (1392) 1083 t MS,2 linalool 1096 (1549) 1095 t MS,2 undecane 1100 1100 t MS,2 cis-thujone 1117 1101 t MS,1 cryptone 1180 (1669) 1183 0.3 MS,1 α-terpineol 1183 (1700) 1186 0.2 MS,1 n-decanal 1202 1201 t MS,2 pulegone 1231 1233 t MS,2 (E,Z)-2,4-decadienal 1301 (1229) 1292 t MS,1 carvacrol 1303 1298 t MS,2 2,4,5-trimethyl benzaldehyde 1305 (1896) -- t MS,1 δ-elemene 1333 (1468) 1335 t MS,2 α-longipinene 1351 1350 t MS,1 α-copaene 1366 (1480) 1374 0.3 MS,2 daucene 1370 1380 t MS,1 β-elemene 1387 (1587) 1389 1.6 MS,2 α-gurjunene 1406 (1528) 1409 t MS,2 (E)-caryophyllene 1410 (1594) 1417 3.0 MS,2 α-trans-bergamotene 1432 (1583) 1432 t MS,1 α-guaiene 1433 1437 t MS,2 aromadendrene 1434 (1605) 1439 0.2 MS,2 α-humulene 1447 (1667) 1452 0.3 MS,2 Separations 2024, 11, 172 8 of 15 Table 3. Cont. Compound I I1it %. IM (E)-β-farnesene 1452 (1770) 1454 0.2 MS,2 β-acoradiene 1456 1469 0.8 MS,1 β-chamigrene 1461 1476 6.0 MS,1 γ-muurolene 1465 (1675) 1478 3.4 MS,2 β-selinene 1482 (1704) 1489 t MS,2 valencene 1485 1496 49.7 MS,2 α-selinene 1492 (1727) 1498 0.1 MS,2 α-bulnesene 1498 (1642) 1509 t MS,2 7-epi-α-selinene 1519 (1764) 1520 0.8 MS,1 δ-cadinene 1522 (1760) 1522 1.0 MS,2 n.i. 1 1559 -- 0.5 -- selina-3,7(11)-diene 1560 1545 0.3 MS,1 caryophyllenyl alcohol 1569 1570 0.2 MS,1 spathulenol 1576 (2133) 1577 1.0 MS,2 caryophyllene oxide 1580 (1987) 1582 0.4 MS,2 globulol 1583 (2064) 1590 0.3 MS,2 viridiflorol 1590 (2091) 1592 0.3 MS,1 carotol 1594 (2026) 1594 0.2 MS,1 β-oplopenone 1597 1607 0.1 MS,1 n.i. 2 1606 -- 0.4 -- epi-α-muurolol (+cubenol) 1631 (1890) 1640 t MS,1 β-eudesmol 1638 (2239) 1649 t MS,2 α-cadinol 1639 (2243) 1652 0.4 MS,1 selin-11-en-4-α-ol 1645 1658 t MS,1 14-hydroxy-9-epi-(E)-caryophyllene 1662 (1924) 1668 t MS,1 khusinol 1665 1679 0.2 MS,1 acorenone 1673 1692 t MS,1 (E)-sesquilavandulyl acetate 1775 1739 t MS,1 cedr-8-(15)-en-9-α-ol acetate 1776 1741 t MS,1 14-hydroxy-α-muurolene 1787 (1550) 1779 0.1 MS,1 notkatone 1798 1806 t MS,1 bis-(2-methylpropyl) phthalate 1804 -- t MS,1 neophytadiene 1810 -- t MS,1 (E,E)-farnesyl acetate 1824 1845 t MS,1 phyllocladene isomer 1857 -- 23.7 MS,1 phytol 1922 (2620) 1942 0.1 MS,1 I = Linear retention indices on DB-1 column and on DB-Wax column in parenthesis; I1it = literature linear retention index on DB-5 column [85]; IM = identification method; MS = mass spectra data; 1 = retention data according to literature values; 2 = retention data according to authentic standards; t = traces (% < 0.1); n.i.= not identified; E.d. = Eryngium galioides; n.i. 1 I = 1559, 220[M+] (35), 135 (100), 107 (88), 159 (85), 91 (83), 121 (81), 177 (79), 81 (60), 41 (45), 55 (40), 137 (39), 69 (30), 161 (23), 205 (20), 189 (10); n.i. 2 I = 1606, 220[M+] (15), 159 (100), 93 (65), 119 (45), 177 (43), 107 (35), 135 (34), 79 (30), 41 (28), 67 (20), 149 (15), 204 (15), 189 (10); Phyllocladene isomer I = 1857, 272[M+] (1), 91 (100), 55 (83), 115 (78), 117 (70), 129 (55), 159 (53), 41 (48), 77 (47), 103 (45), 141 (40), 173 (15), 187 (10), 229 (8), 256 (5). Main compounds on bold. Separations 2024, 11, 172 9 of 15 The Eryngium species studied to date that grow in the Iberian Peninsula show two patterns: E. bourgatii, E. campestre, E. corniculatum, E. glaciale, and E. dilatatum share the same principal compounds in their different fractions, while the root fraction of E. aquifolium and E. maritimum does not have the same components (Tables 1 and 2). As in the case of E. galioides, it would have been interesting to analyze the phenology of its essential oils when it was harvested to check if the season affects its metabolism and the production of terpenoids. The small size of this species did not allow for its fractionation, but one of its main compounds—phyllocladane, or a phyllocladene isomer—is present in the other existing species studies. According to our results and those previously published on the species of Eryngium growing in the Iberian Peninsula, there is no major compound that could be considered as characteristic of the genus. Other compounds that were present in the majority of them were germacrene D, trimethylbenzaldehyde and phyllocladene. However more studies would need to be carried out to determine if any of them or the combination of a few were characteristic of the essential oils of this genus, as well as to determine the phenology of these species and check if the above-mentioned compounds appear in a specific season or biological state of the plant (flowering, fruiting, etc.). The classification of terpenoids present in E. galioides essential oil appears in Figure 2, together with the main compound of each class. The sesquiterpene group (71.2%) was the predominant group, followed by the diterpene group (23.8%). Monoterpenes (0.7%) were insignificant, representing less than 1% of the total oil. In all the groups analyzed (sesquitepenes, diterpenes and monoterpenes, respectively), the hydrocarbon compounds (67.7%, 23.7%, 0.5%) were more abundant than the oxygenated components (3.5%, 0.1%, 0.2%). However, this distribution does not correspond to the number of compounds. Although more than twenty compounds were identified as monoterpenes in the oil, most of them were detected as traces. On the other hand, diterpenes were the second- most-abundant fraction, comprising only two compounds. Regarding sesquiterpenes, this class was the most equitable and showed the highest percentage composition (71.2%) with the largest number of compounds. Our results on the terpenoid fraction agree with those previously reported [17–39]. Most of the Eryngium species studied to date that inhabit open and dry areas have sesquiterpenes as the predominant fraction, whereas the species that grow close to lakes or wet soils have high amounts of monoterpenes [25]. Other species of Eryngium growing under hard climatic conditions exhibited diterpenes as the main components [17–39]. E. campestre seems to modify its chemical composition according to the type of soil [22]. This could also be one of the reasons that none of the compounds described to date for this genus could be used for chemotaxonomy proposes. As we have previously mentioned, different species of this genus have been studied for their biological activities [60–81]. However, only three of them correspond to species that appear in the Iberian Peninsula [10,21,30,42,43,72,75]. Eryngium duriaei subsp. Jure- sianum, from Portugal, showed antifungal activity against several dermatophyte species, with the main compounds identified from the volatiles being α-neocallitropsene (26.0%), isocaryophyllen-14-al (β-Betulenal) (16.2%) and 14-Hydroxy-β-caryophyllened (13.4%) [30]. Moreover, the essential oils also were anti-inflammatory in human chondrocytes [72]. The bio-activity of Eryngium campestre has been also reported. This species, growing in Italy, exhibited cytotoxic activity against tumor cell lines (HCT116 colon carcinoma, breast car- cinoma and melanoma) but weak acetylcholinesterase inhibition [10]. Additionally, its essential oils, from Algeria, presented chemical variability according to the geographical samples studied and inhibited the growth of Gram-positive strains while also exhibiting cytotoxicity activity (anti-leishmanial and anti-trypanosomal) [21]. Finally, the essential oil of the same species, growing in Iran, was demonstrated to be efficient as a preservative in controlling the microbial growth of cherries [61]. The essential oils of the last species, Eryngium maritimum, were also consistently different depending on the material used (leaves, aerial parts, roots or fruits, Table 2) but presented antioxidant and anticorrosive activities. The oils from the Tunisian fruits showed antioxidant activity significantly higher than Trolox [42]. This activity has been also reported for this species growing in Italy and Separations 2024, 11, 172 10 of 15 tested in different fractions (flowers, leaves, stems and roots) [43]. The essential oils of this species growing in France were proposed to serve as an environmentally friendly inhibitor of the corrosion of mild steel in HCl media [75]. As far as we know, this is the first report about Eryngium galioides, and based on the data, we believe it would be interesting to investigate the bio-activities of this species. In fact, valencene, which was detected as the main compound with a percentage com- position close to 50%, has been previously reported to exhibit antimicrobial, antioxidant, anti-inflammatory and antitumor activities [90,91]. The small size of this species and its conservation state are advantages in terms its use for extracting this active compound. However, its in vitro cultivation would make it possible to extract this bio-active in large quantities. To this end, research should be carried out either to propagate this species under laboratory conditions to reinforce the populations more affected, or to generate larger amounts of this plant to make the extraction of essential oils rich in this bio-active possible. Phyllocladane, another major compound of this species, has also been reported for its biological activity [92,93] and, therefore, would contribute to reinforcing the interest in E. galioides as a natural source of bio-actives for different applications. Furthermore, it is accepted that the complex mixtures of the essential oils with bio-active properties have synergy and are more effective than purified bio-active compounds. Therefore, with this report, we contribute to the knowledge of E. galioides, an endemic species of interest. It should be considered a starting point for further studies that can provide a better understanding of this species and its potential bio-active properties. Separations 2024, 11, x FOR PEER REVIEW 10 of 15 Figure 2. Classification of terpenoids in the essential oil of Eryngium galioides Lam. and the main compound of each class (M. = Monoterpenes; S. = Sesquiterpentes; D. = Diterpenes; H. = hydrocar- bons; O. = Oxygenated). As far as we know, this is the first report about Eryngium galioides, and based on the data, we believe it would be interesting to investigate the bio-activities of this species. In fact, valencene, which was detected as the main compound with a percentage composition close to 50%, has been previously reported to exhibit antimicrobial, antioxidant, anti-in- flammatory and antitumor activities [90,91]. The small size of this species and its conser- vation state are advantages in terms its use for extracting this active compound. However, its in vitro cultivation would make it possible to extract this bio-active in large quantities. To this end, research should be carried out either to propagate this species under labora- tory conditions to reinforce the populations more affected, or to generate larger amounts of this plant to make the extraction of essential oils rich in this bio-active possible. Phyllo- cladane, another major compound of this species, has also been reported for its biological activity [92,93] and, therefore, would contribute to reinforcing the interest in E. galioides as a natural source of bio-actives for different applications. Furthermore, it is accepted that the complex mixtures of the essential oils with bio-active properties have synergy and are more effective than purified bio-active compounds. Therefore, with this report, we contribute to the knowledge of E. galioides, an endemic species of interest. It should be considered a starting point for further studies that can provide a better understanding of this species and its potential bio-active properties. 4. Conclusions Figure 2. Classification of terpenoids in the essential oil of Eryngium galioides Lam. and the main com- pound of each class (M. = Monoterpenes; S. = Sesquiterpentes; D. = Diterpenes; H. = hydrocarbons; O. = Oxygenated). Separations 2024, 11, 172 11 of 15 4. Conclusions This is the first report about the chemical composition of the essential oils of E. galioides Lam. from the Iberian Peninsula and represents an advance in the knowledge of this scarcely studied species. The yield of E. galioides essential oil is higher than the average of other Eringium species growing in the Iberian Peninsula. The chemical composition of the essential oils of E. galioides was characterized by two bio-active compounds: valencene (49.7%) and a phyllocladene isomer (23.7%), constituting more than 70% of the total volatiles identified. All these promising features, as regards the production of bio-active essential oils, make research on the in vitro cultivation of this species worthy of consideration to overcome the present limitations of the available number of wild samples. The Iberian Peninsula species of the Erynigum genus, studied to date and growing under comparable conditions, share the predominance of sesquiterpenes in their essential oils, with major components and relative concentrations being dependent on the species considered. The ecological conditions seem to affect, to a noticeable extent, the chemical compo- sition of this genus. Author Contributions: Conceptualization, J.P.-P., M.J.P.-A., A.C.S. and J.J.B.; Methodology, J.P.-P., M.J.P.-A., A.C.S. and J.J.B.; Formal analysis, J.P.-P., R.A.-C., A.C.S. and J.J.B.; Investigation, J.P.-P., M.J.P.-A., R.A.-C., A.C.S. and J.J.B.; Resources, J.P.-P.; Writing—original draft, J.P.-P., A.C.S. and J.J.B.; Writing—review & editing, J.P.-P., R.A.-C., A.C.S. and J.J.B.; Supervision, J.P.-P., A.C.S. and J.J.B.; Project administration, J.P.-P. All authors have read and agreed to the published version of the manuscript. Funding: This research received no external funding. Data Availability Statement: Data are contained within the article. Conflicts of Interest: The authors declare no conflicts of interest. References 1. World Flora Online (‘WFO, 2024). Published on the Internet. Available online: http://www.worldfloraonline.org (accessed on 12 March 2024). 2. Thiviya, P.; Gunawardena, N.; Gamage, A.; Madhujith, T.; Merah, O. 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Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. https://doi.org/10.1021/acs.jafc.2c07543 https://doi.org/10.3390/pharmaceutics15102400 https://pubchem.ncbi.nlm.nih.gov/compound/Phyllocladene https://foodb.ca/compounds/FDB017462 Introduction Materials and Methods Plant Material Isolation of Volatile Oils Gas Chromatography (GC) Gas Chromatography–Mass Spectrometry (GC-MS) Qualitative and Quantitative Analyses Results and Discussion Conclusions References