RT Journal Article
T1 Differential patterns in runs of homozygosity in two mice lines under divergent selection for environmental variability for birth weight
A1 Ojeda Marín, Candela
A1 Gutiérrez García, Juan Pablo
A1 Formoso-Rafferty Castilla, Nora
A1 Goyache Goñi, Félix
A1 Cervantes Navarro, Isabel
AB Runs of homozygosity (ROH) are defined as long continuous homozygous stretches in the genome which are assumed to originate from a common ancestor. It has been demonstrated that divergent selection for variability in mice is possible and that low variability in birth weight is associated with robustness. To analyse ROH patterns and ROH-based genomic inbreeding, two mouse lines that were divergently selected for birth weight variability for 26 generations were used, with: 752 individuals for the high variability line (H-Line), 766 individuals for the low variability line (L-Line) and 74 individuals as a reference population. Individuals were genotyped using the high density Affymetrix Mouse Diversity Genotyping Array. ROH were identified using both the sliding windows (SW) and the consecutive runs (CR) methods. Inbreeding coefficients were calculated based on pedigree (FPED) information, on ROH identified using the SW method (FROHSW) and on ROH identified using the CR method (FROHCR). Differences in genomic inbreeding were not consistent across generations and these parameters did not show clear differences between lines. Correlations between FPED and FROH were high, particularly for FROHSW. Moreover, correlations between FROHSW and FPED were even higher when ROH were identified with no restrictions in the number of heterozygotes per ROH. The comparison of FROH estimates between either of the selected lines were based on significant differences at the chromosome level, mainly in chromosomes 3, 4, 6, 8, 11, 15 and 19. ROH-based inbreeding estimates that were computed using longer homozygous segments had a higher relationship with FPED. Differences in robustness between lines were not attributable to a higher homozygosis in the L-Line, but maybe to the different distribution of ROH at the chromosome level between lines. The analysis identified a set of genomic regions for future research to establish the genomic basis of robustness.
PB Wiley
SN 0931-2668
YR 2023
FD 2023-11-22
LK https://hdl.handle.net/20.500.14352/104470
UL https://hdl.handle.net/20.500.14352/104470
LA eng
NO Ojeda-Marín, C.,Gutiérrez, J. P., Formoso-Rafferty, N., Goyache, F.,& Cervantes, I. (2024). Differential patterns in runsof homozygosity in two mice lines under divergentselection for environmental variability for birthweight. Journal of Animal Breeding and Genetics,141, 193–206. https://doi.org/10.1111/jbg.12835
NO 2023 Acuerdos transformativos CRUE
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 21 dic 2025