%0 Journal Article
%A Jiménez Holguín, Javier
%A Lozano Borregón, Daniel
%A Saiz-Pardo Sanz, Melchor
%A Pablo, David de
%A Ortega Medina, Luis
%A Portolés Pérez, María Teresa
%A Arcos Navarrete, Daniel
%T Bone regeneration in sheep model induced by strontium-containing mesoporous bioactive glasses
%D 2025
%U https://hdl.handle.net/20.500.14352/115297
%X Local delivery of therapeutic ions from bioactive mesoporous glasses (MBGs) is postulated as one of the most promising strategies for regenerative therapy of critical bone defects. Among these ions, Sr2+ cation has been widely considered for this purpose as part of the composition of MBGs. MBGs of chemical composition 75SiO2- 25-x CaO-5P2O5-xSrO with x = 0, 2.5 and 5 (% mol) were prepared by the evaporation induced self-assembly (EISA) method. Strontium incorporation did not affect the apatite forming ability of Sr-free MBG when these bioceramics are treated with simulated body fluid (SBF). In vitro cell viability showed that proliferation of MC3T3-E1 preosteoblast is not affected by the presence of Sr2+ cations, whereas ALP activity and gene expression of Runx2, ALP and VEGF is increased as a function of Sr content. Besides, cell proliferation and VEGF expression of HUVEC cells were also increased with the Sr2+ content. In this work, we present for the first time the effects of Sr containing MBGs on bone regeneration in a large animal model (sheep), after implantation in a cavitary defect. The histomorphometrical analysis and immunohistochemistry indicate that the incorporation of Sr2+ ion greatly enhances the osteoregenerating potential of MBGs. In this sense, the measured ossification areas were 7 % and 20 % for MBG and Sr-MBG, respectively. Besides, the thickness of the newly formed trabeculae was 15 μm and 30 μm for MBG and Sr-MBG, respectively. This enhancement of Sr2+ mediated bone formation would be justified by the transient osteoclastogenesis inhibition and the osteogenesis-angiogenesis increase due to the endothelial cell proliferation and increased vascular endothelial growth factor expression.
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