RT Journal Article
T1 P‐selectin glycoprotein ligand‐1 modulates immune inflammatory responses in the enteric lamina propria
A1 Nuñez‐Andrade, Norman
A1 Lamana Domínguez, Amalia
A1 Sancho, David
A1 Gisbert, Javier
A1 Gonzalez‐Amaro, Roberto
A1 Sanchez‐Madrid, Francisco
A1 Urzainqui, Ana
AB P-selectin glycoprotein ligand-1 (PSGL-1), a leukocyte adhesion receptor that interacts with selectins, induces a tolerogenic programme in bone marrow-derived dendritic cells (DCs), which in turn promotes the generation of T regulatory (Treg) lymphocytes. In the present study, we have used a mouse model of dextran sulphate sodium (DSS)-induced colitis and studied the characteristics of the inflammatory cell infiltrate in the lamina propria (LP), mesenteric lymph nodes (mLNs) and Peyer's patches (PPs) to assess the possible role of PSGL-1 in the modulation of the enteric immune response. We have found that untreated PSGL-1-deficient mice showed an altered proportion of innate and adaptive immune cells in mLNs and PPs as well as an activated phenotype of macrophages and DCs in the colonic LP that mainly produced pro-inflammatory cytokines. Administration of an anti-PSGL-1 antibody also reduced the total numbers of macrophages, DCs and B cells in the colonic LP, and induced a lower expression of MHC-II by DCs and macrophages. After DSS treatment, PSGL-1−/− mice developed colitis earlier and with higher severity than wild-type (WT) mice. Accordingly, the colonic LP of these animals showed an enhanced number of Th1 and Th17 lymphocytes, with enhanced synthesis of IL-1α, IL-6 and IL-22, and increased activation of LP macrophages. Together, our data indicate that PSGL-1 has a relevant homeostatic role in the gut-associated lymphoid tissue under steady-state conditions, and that this adhesion receptor is able to down-regulate the inflammatory phenomenon in DSS-induced colitis. Copyright © 2011 Pathological Society of Great Britain and Ireland.
PB Wiley
SN 0022-3417
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/94322
UL https://hdl.handle.net/20.500.14352/94322
LA eng
NO Nuñez‐Andrade, Norman, et al. «P‐selectin Glycoprotein Ligand‐1 Modulates Immune Inflammatory Responses in the Enteric Lamina Propria». The Journal of Pathology, vol. 224, n.o 2, junio de 2011, pp. 212-21. https://doi.org/10.1002/path.2850.
NO We thank the CNIC and UAM animal facilities for animal breeding and technical support, the CNIC histology and cytometry services for technical support, and S. Bartlett for English editing. This study was supported by the Spanish Ministry of Health and Consumer Affairs (Grant No. FIS-PI080894) to AU, and the Comunidad Autónoma de Madrid (INSINET Grant No. SAF2008) and FONCICYT (Grant No. C002-2009.1ALA1/127249) to FS-M.
NO Consejo Nacional de Humanidades, Ciencias y Tecnologías (México)
NO Comunidad de Madrid
NO Ministerio de Sanidad (España)
DS Docta Complutense
RD 14 dic 2025