RT Journal Article
T1 Differential Apoptotic Effects of Bee Product Mixtures on Normal and Cancer Hepatic Cells
A1 Sánchez Martín, Vanesa
A1 Morales Gómez, Paloma
A1 Iriondo De Hond, Amaia
A1 Fernández Hospital, Xavier
A1 Fernández Álvarez, Manuela
A1 Hierro Paredes, Eva
A1 Haza Duaso, Ana Isabel
A2 Gan, Ren-You
AB Most effective anticancer drugs normally generate considerable cytotoxicity in normal cells; therefore, the preferential activation of apoptosis in cancer cells and the reduction of toxicity in normal cells is a great challenge in cancer research. Natural products with selective anticancer properties used as complementary medicine can help to achieve this goal. The aim of the present study was to analyze the effect of the addition of bee products [propolis (PR) or royal jelly (RJ) or propolis and royal jelly (PR+RJ), 2–10%] to thyme (TH) and chestnut honeys (CH) on the differential anticancer properties, mainly the cytotoxic and pro-apoptotic effects, in normal and cancer hepatic cells. The cytotoxic effects of samples were analyzed using the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay (0–250 mg/mL) and the effects on apoptosis were analyzed using cell cycle analysis, TdT-dUTP terminal nick-end labeling (TUNEL) assay, DR5 (Death Receptor 5) and BAX (BCL-2-Associated X) activation, and caspases 8, 9, and 3 activities. Both honey samples alone and honey mixtures had no or very little apoptotic effect on normal cells. Antioxidant honey mixtures enhanced the apoptotic capacity of the corresponding honey alone via both extrinsic and intrinsic pathways. Of all the samples, chestnut honey enriched with 10% royal jelly and 10% propolis (sample 14, CH+10RJ+10PR) showed the highest apoptotic effect on tumor liver cells. The enrichment of monofloral honey with bee products could be used together with conventional anticancer treatments as a dietary supplement without side effects. On the other hand, it could be included in the diet as a natural sweetener with high added value.
PB MDPI
YR 2023
FD 2023-03-02
LK https://hdl.handle.net/20.500.14352/91544
UL https://hdl.handle.net/20.500.14352/91544
LA eng
NO Sánchez-Martín, V.; Morales, P.; Iriondo-DeHond, A.; Hospital, X.F.; Fernández, M.; Hierro, E.; Haza, A.I. Differential Apoptotic Effects of Bee Product Mixtures on Normal and Cancer Hepatic Cells. Antioxidants 2023, 12, 615. https:// doi.org/10.3390/antiox12030615
NO Ministerio de Ciencia e Innovación
DS Docta Complutense
RD 28 jul 2024