RT Journal Article
T1 Adenovirus-vectored African Swine Fever Virus Antigens Cocktail Is Not Protective against Virulent Arm07 Isolate in Eurasian Wild Boar
A1 Cadenas Fernández, Estefanía
A1 Sánchez-Vizcaíno Rodríguez, José Manuel
A1 Kosowska, Aleksandra
A1 Rivera Arroyo, Belén
A1 Mayoral Alegre, Francisco José
A1 Rodríguez Bertos, Antonio Manuel
A1 Yao, Jianxiu
A1 Bray, Jocelyn
A1 Lokhandwala, Shehnaz
A1 Mwangi, Waithaka
A1 Barasona García-Arévalo, José Ángel
AB African swine fever (ASF) is a viral disease of domestic and wild suids for which there is currently no vaccine or treatment available. The recent spread of ASF virus (ASFV) through Europe and Asia is causing enormous economic and animal losses. Unfortunately, the measures taken so far are insufficient and an effective vaccine against ASFV needs to be urgently developed. We hypothesized that immunization with a cocktail of thirty-five rationally selected antigens would improve the protective efficacy of subunit vaccine prototypes given that the combination of fewer immunogenic antigens (between 2 and 22) has failed to elicit protective efficacy. To this end, immunogenicity and efficacy of thirty-five adenovirus-vectored ASFV antigens were evaluated in wild boar. The treated animals were divided into different groups to test the use of BioMize adjuvant and different inoculation strategies. Forty-eight days after priming, the nine treated and two control wild boar were challenged with the virulent ASFV Arm07 isolate. All animals showed clinical signs and pathological findings consistent with ASF. This lack of protection is in line with other studies with subunit vaccine prototypes, demonstrating that there is still much room for improvement to obtain an effective subunit ASFV vaccine.
PB MDPI
SN 2076-0817
YR 2020
FD 2020-02-28
LK https://hdl.handle.net/20.500.14352/8058
UL https://hdl.handle.net/20.500.14352/8058
LA eng
NO Unión Europea. Horizonte 2020
NO Ministerio de Economía y Competitividad (MINECO)
NO National Institute of Food and Agriculture (USDA)/Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD)
DS Docta Complutense
RD 15 ago 2024