%0 Journal Article
%A Reche Gallardo, Pedro Antonio
%A Soumelis, Vassili
%A Gorman, D M
%A Clifford, T
%A Liu, MR
%A Travis, M
%A Zurawski, Sandra M.
%A Johnston, J
%A Liu, Yong-Jun
%A Spits, H
%A Waal-Malefyt, Rene de
%A Kastelein, Robert A.
%A Bazan, Fernando
%T Human thymic stromal lymphopoietin preferentially stimulates myeloid cells
%D 2001
%@ 0022-1767
%U https://hdl.handle.net/20.500.14352/58252
%X The sequence of a novel hemopoietic cytokine was discovered in a computational screen of genomic databases, and its homology to mouse thymic stromal lymphopoietin (TSLP) suggests that it is the human orthologue. Human TSLP is proposed to signal through a heterodimeric receptor complex that consists of a new member of the hemopoietin family termed human TSLP receptor and the IL-7R alpha-chain. Cells transfected with both receptor subunits proliferated in response to purified, recombinant human TSLP, with induced phosphorylation of Stat3 and Stat5. Human TSLPR and IL-7Ralpha are principally coexpressed on monocytes and dendritic cell populations and to a much lesser extent on various lymphoid cells. In accord, we find that human TSLP functions mainly on myeloid cells; it induces the release of T cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells, as evidenced by the strong induction of the costimulatory molecules CD40 and CD80 and the enhanced capacity to elicit proliferation of naive T cells.
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