RT Journal Article
T1 Reagentless fluorescent biosensors based on proteins for continuous monitoring systems
A1 Galbán, Javier
A1 Sanz-Vicente, Isabel
A1 Ortega, Estefanía
A1 Del Barrio Redondo, Melisa
A1 de Marcos, Susana
AB There is a lack of commercially available efficient and autonomous systems capable of continuous monitoring of (bio)chemical data for clinical, environmental, food, or industrial samples. The weakest link in the design of these systems is the (bio)chemical receptor (bCR). The bCR should have transducer ability, the recognition event should be a single reaction, and the bCR should be easily regenerated. Transport proteins and enzymes are well placed as bCR for optical continuous monitoring systems (OCMS). In this paper we review quantitative aspects and the main transducer strategies which have been developed for transport proteins, using periplasmic binding proteins (linking an environmentally sensitive fluorophore or FRET between two fluorophores) and concanavalin A (competitive reversible assays) as representative examples. Efficient immobilization systems and implementation in OCMS are also reviewed. Some kinds of enzymes can fulfil the necessary requirements to be appropriate bCR. Strategies using flavoenzymes chemically modified with fluorophores can be successfully implemented in OCMS and they are, in our opinion, the most appropriate option
PB Springer
SN 1618-2650
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/93841
UL https://hdl.handle.net/20.500.14352/93841
LA eng
NO Galbán, J., Sanz-Vicente, I., Ortega, E. et al. Reagentless fluorescent biosensors based on proteins for continuous monitoring systems. Anal Bioanal Chem 402, 3039–3054 (2012). https://doi.org/10.1007/s00216-012-5715-2
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 10 abr 2025