RT Journal Article
T1 Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa
A1 Sánchez Calabuig, María Jesús
A1 de la Fuente, Julio
A1 Laguna Barraza, Ricardo
A1 Beltrán-Breña, Paula
A1 Martínez Nevado, Eva
A1 Johnston, Steve D
A1 Rizos, Dimitrios
A1 Gutiérrez-Adán, Alfonso
A1 Pérez Gutiérrez, José Félix
AB Assisted reproductive technologies are of great importance for increasing the genetic diversity in captive animals. The use of bovine or murine oocytes in heterologous IVF provides advantages compared to homologous IVF in nondomestic animals, such as the accessibility to oocytes and the availability of well-developed in vitro maturation systems. The aim of this study was to determine the heterologous IVF parameters using cryopreserved dolphin spermatozoa and zona-intact bovine or murine oocytes and to examine the nuclear chromatin status of the dolphin spermatozoa. All the processes involved in the fertilization including embryo cleavage were observed by confocal microscopy and hybrid embryo formation was confirmed by polymerase chain reaction. Heterologous bovine IVF showed no polyspermy, lower percentages of pronuclear formation, and a lower cleavage rate compared to homologous IVF group (34.8% vs. 89.3%). Heterologous murine IVF showed a lower cleavage rate than homologous IVF (9.6% vs. 77.1%). With respect to dolphin sperm chromatin, it was more stable, i.e. more resistant to EDTA-SDS decondensation than the bovine sperm chromatin. This study revealed the stability of the dolphin sperm chromatin and the ability of the dolphin spermatozoa to penetrate zona-intact bovine and murine oocytes, leading to hybrid embryo formation.
PB Elsevier
SN 0093-691X
YR 2015
FD 2015-10-01
LK https://hdl.handle.net/20.500.14352/117889
UL https://hdl.handle.net/20.500.14352/117889
LA eng
NO Sánchez-Calabuig, de la Fuente, Laguna-Barraza, Beltrán-Breña, Martínez-Nevado, Johnston, Rizos, Gutiérrez-Adán, & Pérez-Gutiérrez. (2015). Heterologous murine and bovine IVF using bottlenose dolphin (Tursiops truncatus) spermatozoa. Theriogenology, 84(6), 983-994. https://doi.org/10.1016/J.THERIOGENOLOGY.2015.06.001
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 8 abr 2025