RT Journal Article
T1 Xanthohumol exerts protective effects in liver alterations associated with aging
A1 Fernández-García, Cristina
A1 Rancan, Lisa
A1 Paredes Royano, Sergio Damián
A1 Montero, César
A1 Fuente del Rey, Mónica de la
A1 Vara Ameigeiras, Elena
A1 Tresguerres, Jesús A.
AB Background and aimsAging is associated with a deregulation of biological systems that lead to an increase in oxidative stress, inflammation, and apoptosis, among other effects. Xanthohumol is the main preylated chalcone present in hops (Humulus lupulus L.) whose antioxidant, anti-inflammatory and chemopreventive properties have been shown in recent years. In the present study, the possible protective effects of xanthohumol on liver alterations associated with aging were evaluated.MethodsMale young and old senescence-accelerated prone mice (SAMP8), aged 2 and 10 months, respectively, were divided into four groups: non-treated young, non-treated old, old treated with 1 mg/kg/day xanthohumol, and old treated with 5 mg/kg/day xanthohumol. Male senescence-accelerated resistant mice (SAMR1) were used as controls. After 30 days of treatment, animals were sacrificed and livers were collected. mRNA (AIF, BAD, BAX, Bcl-2, eNOS, HO-1, IL-1β, NF-κB2, PCNA, sirtuin 1 and TNF-α) and protein expressions (BAD, BAX, AIF, caspase-3, Blc-2, eNOS, iNOS, TNF-α, IL1β, NF-κB2, and IL10) were measured by RT-PCR and Western blotting, respectively. Mean values were analyzed using ANOVA.ResultsA significant increase in mRNA and protein levels of oxidative stress, pro-inflammatory and proliferative markers, as well as pro-apoptotic parameters was shown in old non-treated SAMP8 mice compared to the young SAMP8 group and SAMR1 mice. In general, age-related oxidative stress, inflammation and apoptosis were significantly decreased (p < 0.05) after XN treatment. In most cases, this effect was dose-dependent.ConclusionsXN was shown to modulate inflammation, apoptosis, and oxidative stress in aged livers, exerting a protective effect in hepatic alterations.
PB Springer
SN 1436-6215
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/13585
UL https://hdl.handle.net/20.500.14352/13585
LA eng
NO Universidad Complutense de Madrid / Banco Santander Central Hispano (BSCH)
NO Red de Fragilidad y Envejecimiento (RETICEF)
DS Docta Complutense
RD 8 may 2024