RT Journal Article T1 Enhancing felid conservation: Exploring the impact of in vitroculture media on domestic cat blastocyst production A1 Muñoz Maceda, Ana A1 Priego González, Andrea A1 Núñez Puente, Carolina A1 Rizos, Dimitrios A1 Cerdeira Lozano, Joaquín Vicente A1 Roldán, Eduardo A1 Sánchez Calabuig, María Jesús A1 Sánchez Rodríguez, Ana AB This study investigated the optimization of assisted reproductive techniques for wild felid conservation, focusing on in vitro procedures using the domestic cat as a model species. The research evaluated the impact of three different in vitro culture media on blastocyst formation. Oocytes and spermatozoa were collected and processed, followed by in vitro fertilization and culture. Results returned a similar blastocyst rate (ANOVA, p > .05), over 16% across all groups. While demonstrating the potential of these techniques, further investigations are warranted to evaluate embryo quality to refine optimal protocols and their applicability in felid conservation efforts. PB Wiley SN 0936-6768 YR 2024 FD 2024 LK https://hdl.handle.net/20.500.14352/109804 UL https://hdl.handle.net/20.500.14352/109804 LA eng NO Munoz-Maceda, A., Priego-Gonzalez, A., Núñez-Puente, C., Rizos, D., Cerdeira-Lozano, J., Sanchez-Rodriguez, A., Roldan, E. R. S., & Sánchez-Calabuig, M. J. (2024). Enhancing felid conservation: Exploring the impact of in vitro culture media on domestic cat blastocyst production. Reproduction in Domestic Animals, 59(Suppl. 3), e14645. https://doi.org/10.1111/rda.14645 NO Comunidad de Madrid NO Museo Nacional de Ciencias Naturales (España) NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España) DS Docta Complutense RD 21 abr 2025