RT Journal Article
T1 Associating Growth-Phase-Related Changes in the Proteome of Acinetobacter baumannii with Increased Resistance to Oxidative Stress
A1 Soares, Nelson C.
A1 Cabral, Maria P.
A1 Gayoso, Carmen
A1 Mallo, Susana
A1 Rodriguez Velo, Patricia
A1 Fernández Moreira, Esteban
A1 Bou, German
AB Acinetobacter baumannii is an opportunistic pathogen that has been associated with severe infections and outbreaks in hospitals. At present, very little is known about the biology of this bacterium, particularly as regards mechanisms of adaptation, persistence and virulence. To investigate the growth phase-dependent regulation of proteins in this microorganism, we analyzed the proteomic pattern of A. baumannii ATCC 17978 at different stages of in vitro growth. In this study, proteomics analyses were conducted using 2-DE and MALDI-TOF/TOF complemented by iTRAQ LC-MS/MS. Here we have identified 107 differentially expressed proteins. We highlight the induction of proteins associated with signaling, putative virulence factors and response to stress (including oxidative stress). We also present evidence that ROS (O(2)(-) and OH(-)) and RNI (ONOO(-)) accumulate during late stages of growth. Further assays demonstrated that stationary cells survive at high concentrations of H(2)O(2) (30 mM), the O(2)(-) donor menadione (500 muM) or the NO donor sodium nitroprusside (1 mM), and showed a higher survival rate against several bactericidal antibiotics. The growth phase-dependent changes observed in the A. baumannii proteome are discussed within a context of adaptive biological responses, including those related to ROS and RNI stress
PB American Chemical Society
YR 2010
FD 2010-01-28
LK https://hdl.handle.net/20.500.14352/124975
UL https://hdl.handle.net/20.500.14352/124975
LA eng
NO Soares, Nelson C., et al. «Associating Growth-Phase-Related Changes in the Proteome of Acinetobacter Baumannii with Increased Resistance to Oxidative Stress». Journal of Proteome Research, vol. 9, n.o 4, abril de 2010, pp. 1951-64. https://doi.org/10.1021/pr901116r.
NO Instituto de Salud Carlos III-FEDER
NO Xunta de Galicia
DS Docta Complutense
RD 14 jun 2026