RT Journal Article
T1 The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi
A1 Calderón, Jossela
A1 Maganto-Garcia, Elena
A1 Punzón, Carmen
A1 Terhorst, Cox
A1 Fresno, Manuel
A1 Carrión Herrero, Francisco Javier
A2 Sacks, David L.
AB Trypanosoma cruzi, the protozoan parasite responsible for Chagas' disease, causes severe myocarditis often resulting in death. Here, we report that Slamf1-/- mice, which lack the hematopoietic cell surface receptor Slamf1, are completely protected from an acute lethal parasite challenge. Cardiac damage was reduced in Slamf1-/- mice compared to wild type mice, infected with the same doses of parasites, as a result of a decrease of the number of parasites in the heart even the parasitemia was only marginally less. Both in vivo and in vitro experiments reveal that Slamf1-defIcient myeloid cells are impaired in their ability to replicate the parasite and show altered production of cytokines. Importantly, IFN-γ production in the heart of Slamf1 deficient mice was much lower than in the heart of wt mice even though the number of infiltrating dendritic cells, macrophages, CD4 and CD8 T lymphocytes were comparable. Administration of an anti-Slamf1 monoclonal antibody also reduced the number of parasites and IFN-γ in the heart. These observations not only explain the reduced susceptibility to in vivo infection by the parasite, but they also suggest human Slamf1 as a potential target for therapeutic target against T. cruzi infection.
SN 1553-7374
YR 2012
FD 2012-07-12
LK https://hdl.handle.net/20.500.14352/93867
UL https://hdl.handle.net/20.500.14352/93867
LA eng
NO Calderón J, Maganto-Garcia E, Punzón C, Carrión J, Terhorst C, Fresno M (2012) The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi. PLoS Pathog 8(7): e1002799. https://doi.org/10.1371/journal.ppat.1002799
NO Author ContributionsConceived and designed the experiments: J. Calderon, E. Maganto-Garcia, M. Fresno. Performed the experiments: J. Calderon, E. Maganto-Garcia, C. Punzon, J. Carrion. Analyzed the data: J. Calderon, E. Maganto-Garcia, C. Terhorst, M. Fresno. Contributed reagents/materials/analysis tools: C. Terhorst. Wrote the paper: C. Terhorst, M. Fresno.
NO Ministerio de Ciencia e Innovación
NO SAF2005-02220,
NO SAF2007-61716
NO SAF2010-18733
NO CSIC-CONICET
NO BSCH/UAM
NO Comunidad de Madrid
NO S2010/BMD-2332
NO RED RECAVA
NO RED RICET
NO RD06/0014/1013
NO RD06/0021/0016
DS Docta Complutense
RD 22 abr 2025