RT Journal Article
T1 A Proteomic Approach to Determine Changes in Proteins Involved in the Myocardial Metabolism in Left Ventricles of Spontaneously Hypertensive Rats
A1 Zamorano León, José Javier
A1 Modrego, Javier
A1 Mateos Cáceres, Petra
A1 Macaya Miguel, Carlos
A1 Martín Fernández, Beatriz
A1 Miana, María
A1 Heras Jiménez, Natalia De Las
A1 Cachofeiro Ramos, María Victoria
A1 Lahera Julia, Vicente
A1 López Farre, Antonio José
AB Different works have suggested that in the hypertrophied heart the energy metabolic pathway shifts to glycolysis. Our aim was to evaluate using proteomics the expression of proteins associated with different energetic metabolic pathways in hypertrophied left ventricles of spontaneously hypertensive rats (SHR). Methods: 24-weeks-old SHR with stable hypertension and established left ventricle hypertrophy were used. Normotensive Wistar Kyoto rats were used as control. Proteins from left ventricles were analyzed by 2-dimensional electrophoresis and identified by comparison with a virtual rat heart proteomic map and mass spectrometry. Results: Enoyl-CoA hydratase expression, an enzyme involved in fatty acid β-oxidation, was reduced whereas the expression of other β-oxidation enzymes, 3-ketoacyl-CoA thiolase and the mitochondrial precursor of acyl-CoA thioester hydrolase, was increased in the hypertrophied left ventricles. The expression of two enzymes involved in the first steps of glycolysis, fructose bisphosphate aldolase and triosephosphate isomerase, was reduced in the left ventricle of SHR. Pyruvate dehydrogenase expression, enzyme involved in glucose oxidation, was enhanced in the hypertrophied ventricles whereas proteins of the tricarboxylic acid cycle were not modified. Proteins involved in the mitochondrial oxidative phosphorylation were overexpressed whereas the α-subunit of the mitochondrial precursor of ATP synthase was downexpressed. Conclusions: Several proteins involved in the main energy metabolic pathways were up and downexpressed. Moreover, our results seem to suggest that probably neither fatty acid β-oxidation nor glycolysis are the only sources for energy in the hypertrophied left ventricle.
PB S. Karger AG
YR 2010
FD 2010-07-01
LK https://hdl.handle.net/20.500.14352/99316
UL https://hdl.handle.net/20.500.14352/99316
LA eng
NO Zamorano-León JJ, Modrego J, Mateos-Cáceres PJ, Macaya C, Martín-Fernández B, Miana M, de las Heras N, Cachofeiro V, Lahera V, López-Farré AJ. A proteomic approach to determine changes in proteins involved in the myocardial metabolism in left ventricles of spontaneously hypertensive rats. Cell Physiol Biochem. 2010;25(2-3):347-58. doi: 10.1159/000276567. Epub 2010 Jan 12. PMID: 20110695.
NO Ministerio de Sanidad y Consumo
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 31 dic 2025