RT Journal Article
T1 One Pot Use of Combilipases for Full Modification of Oils and Fats: Multifunctional and Heterogeneous Substrates
A1 Arana-Peña, Sara
A1 Carballares, Diego
A1 Berenguer-Murcia, Ángel
A1 Alcántara León, Andrés Rafael
A1 Rodrigues, Rafael
A1 Fernandez-Lafuente, Roberto
AB Lipases are among the most utilized enzymes in biocatalysis. In many instances, the main reason for their use is their high specificity or selectivity. However, when full modification of a multifunctional and heterogeneous substrate is pursued, enzyme selectivity and specificity become a problem. This is the case of hydrolysis of oils and fats to produce free fatty acids or their alcoholysis to produce biodiesel, which can be considered cascade reactions. In these cases, to the original heterogeneity of the substrate, the presence of intermediate products, such as diglycerides or monoglycerides, can be an additional drawback. Using these heterogeneous substrates, enzyme specificity can promote that some substrates (initial substrates or intermediate products) may not be recognized as such (in the worst case scenario they may be acting as inhibitors) by the enzyme, causing yields and reaction rates to drop. To solve this situation, a mixture of lipases with different specificity, selectivity and differently affected by the reaction conditions can offer much better results than the use of a single lipase exhibiting a very high initial activity or even the best global reaction course. This mixture of lipases from different sources has been called “combilipases” and is becoming increasingly popular. They include the use of liquid lipase formulations or immobilized lipases. In some instances, the lipases have been coimmobilized. Some discussion is offered regarding the problems that this coimmobilization may give rise to, and some strategies to solve some of these problems are proposed. The use of combilipases in the future may be extended to other processes and enzymes.
PB MDPI
SN 2073-4344
YR 2020
FD 2020-05-29
LK https://hdl.handle.net/20.500.14352/8314
UL https://hdl.handle.net/20.500.14352/8314
LA eng
NO Ministerio de Ciencia e Innovación (MICIIN)
NO Generalitat Valenciana
DS Docta Complutense
RD 9 abr 2025