RT Journal Article
T1 Analysis of Parainflammation in Chronic Glaucoma Using Vitreous-OCT Imaging
A1 García Herranz, David
A1 Bravo Osuna, Irene
A1 García Feijoo, Julián
A1 Herrero Vanrell, María Del Rocío
A1 Rodrigo, María Jesús
A1 Subías, Manuel
A1 Montolío, Alberto
A1 Méndez-Martínez, Silvia
A1 Martínez-Rincón, Teresa
A1 Arias, Lorena
A1 Pablo, Luis E
A1 Cegoñino, José
A1 Carretero, Ana
A1 Ruberte, Jesús
A1 García-Martín, Elena
A1 Pérez del Palomar, Amaya
AB Glaucoma causes blindness due to the progressive death of retinal ganglion cells. The immune response chronically and subclinically mediates a homeostatic role. In current clinical practice, it is impossible to analyse neuroinflammation non-invasively. However, analysis of vitreous images using optical coherence tomography detects the immune response as hyperreflective opacities. This study monitors vitreous parainflammation in two animal models of glaucoma, comparing both healthy controls and sexes over six months. Computational analysis characterizes in vivo the hyperreflective opacities, identified histologically as hyalocyte-like Iba-1+ (microglial marker) cells. Glaucomatous eyes showed greater intensity and number of vitreous opacities as well as dynamic fluctuations in the percentage of activated cells (50–250 microns2) vs. non-activated cells (10–50 microns2), isolated cells (10 microns2) and complexes (>250 microns2). Smaller opacities (isolated cells) showed the highest mean intensity (intracellular machinery), were the most rounded at earlier stages (recruitment) and showed the greatest change in orientation (motility). Study of vitreous parainflammation could be a biomarker of glaucoma onset and progression.
PB MDPI
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/117981
UL https://hdl.handle.net/20.500.14352/117981
LA eng
NO Rodrigo, M.J.; Subías, M.; Montolío, A.; Méndez-Martínez, S.; Martínez-Rincón, T.; Arias, L.; García-Herranz, D.; Bravo-Osuna, I.; Garcia-Feijoo, J.; Pablo, L.; et al. Analysis of Parainflammation in Chronic Glaucoma Using Vitreous-OCT Imaging. Biomedicines 2021, 9, 1792. https://doi.org/ 10.3390/biomedicines9121792
NO Ministerio de Economía y Competitividad (España)
NO Universidad Complutense de Madrid
NO Instituto de Salud Carlos III
NO Hospital Universitario Rio Hortega (Valladolid)
DS Docta Complutense
RD 4 abr 2025