RT Journal Article
T1 Multiplexed determination of human growth hormone and prolactin at a label free electrochemical immunosensor using dual carbon nanotube–screen printed electrodes modified with gold and PEDOT nanoparticles
A1 Serafín González-Carrato, Verónica
A1 Martínez-García, Gonzalo
A1 Agüí Chicharro, María Lourdes
A1 Yáñez-Sedeño Orive, Paloma
A1 Pingarrón Carrazón, José Manuel
AB A label-free dual electrochemical immunosensor was constructed for the multiplexed determination of human growth (hGH) and prolactin (PRL) hormones. The immunosensor used an electrochemical platform composed of carbon nanotube–screen printed carbon electrodes (CNT/SPCEs) modified with poly(ethylene-dioxythiophene) (PEDOT) and gold nanoparticles, on which the corresponding hGH and PRL antibodies were immobilized. The affinity reactions were monitored by measuring the decrease in the differential pulse voltammetric oxidation response of the redox probe dopamine. The experimental variables involved in the preparation of both AuNP/PEDOT/CNT/SPC modified electrodes and the dual immunosensor were optimized. The immunosensor exhibited an improved analytical performance for hGH and PRL with respect to other electrochemical immunosensor designs, showing wide ranges of linearity and low detection limits of 4.4 and 0.22 pg mL−1, respectively. An excellent selectivity against other hormones and in the presence of ascorbic and uric acids was found. The usefulness of the dual immunosensor for the simultaneous analysis of hGH and PRL was demonstrated by analyzing human serum and saliva samples spiked with the hormones at different concentration levels.
PB Royal Society of Chemistry
SN 0003-2654
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/96868
UL https://hdl.handle.net/20.500.14352/96868
LA eng
NO Serafín, V., et al. «Multiplexed Determination of Human Growth Hormone and Prolactin at a Label Free Electrochemical Immunosensor Using Dual Carbon Nanotube–Screen Printed Electrodes Modified with Gold and PEDOT Nanoparticles». The Analyst, vol. 139, n.o 18, 2014, pp. 4556-63. https://doi.org/10.1039/C4AN00221K.
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 7 abr 2025