RT Journal Article
T1 Modulation of pulmonary fibrosis by pulmonary surfactant-associated phosphatidylethanolamine in vitro and in vivo
A1 Tlatelpa Romero, Beatriz
A1 Vázquez de Lara Cisneros, Luis G.
A1 Cañadas Benito, Olga
A1 Blanco Rivero, Amaya
A1 Olmeda Lozano, Bárbara
A1 Pérez Gil, Jesús
A1 Mendoza Milla, Criselda
A1 Martinez Vaquero, José Luis
A1 Romero, Yair
A1 Contreras Cruz, David Atahualpa
A1 de la Rosa Paredes, René
A1 Ruiz Salgado, Sinuhé
A1 Berra Romani, Roberto
A1 Collantes Gutiérrez, Alonso Antonio
A1 Pérez Fernández, María Susana
A1 Hernández Linares, María Guadalupe
A1 Guerrero Luna, Gabriel
AB Pulmonary fibrosis (PF) is characterized by excessive collagen deposition and impaired lung function. Pulmonary surfactant may modulate fibroblast activity and offer therapeutic benefits. We developed a natural porcine pulmonary surfactant (NPPS) enriched with 1,2-dipalmitoyl-rac-glycero-3-phosphatidylethanolamine (PE) and evaluated its biophysical and biological properties. Biophysical analysis showed that PE improved surfactant performance by increasing surface pressure and stability. In vitro, NPPS-PE reduced collagen expression and induced apoptosis in normal human lung fibroblasts; in addition, it decreased proliferation in fibroblasts stimulated with TGF-β. In vivo, NPPS-PE improved gas exchange and significantly reduced collagen deposition in bleomycin-treated mice. These findings suggest that NPPS-PE may be a promising therapeutic strategy for fibrosing lung diseases.
PB MDPI
SN 1661-6596
YR 2025
FD 2025-07
LK https://hdl.handle.net/20.500.14352/124300
UL https://hdl.handle.net/20.500.14352/124300
LA eng
NO Tlatelpa-Romero, B.; Vázquez-de-Lara Cisneros, L.G.; Cañadas, O.; Blanco-Rivero, A.; Olmeda, B.; Pérez-Gil, J.; Mendoza-Milla, C.; Martinez-Vaquero, J.L.; Romero, Y.; Contreras-Cruz, D.A.; et al. Modulation of Pulmonary Fibrosis by Pulmonary Surfactant-Associated Phosphatidylethanolamine In Vitro and In Vivo. Int. J. Mol. Sci. 2025, 26, 7132. https://doi.org/10.3390/ijms26157132
NO Funding:Part of this work was funded by the Spanish Ministry of Science and Innovation (grant PID2021-124932OB-I00 to O.C., B.O., A.B.-R. and J.P.-G.).
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 30 dic 2025