RT Journal Article T1 Genetics unveil the genealogical ancestry and physical appearance of an unknown historical figure: lady Leonor of Castile (Spain) (1256–1275) A1 Palomo Díez, Sara A1 Lopes Gomes, Claudia Filipa A1 Fondevila, María Sonia A1 Esparza Arroyo, Ángel A1 López Parra, Ana María A1 Lareu, María Victoria A1 Arroyo Pardo, Eduardo A1 Pastor, Juan Francisco AB Through this study, it has been possible to establish an accurate prediction of the physical characteristics, biogeographical origin, and genealogical ancestry of a previously obscured historical figure: The Princess Lady Leonor of Castile (1256–1275), one of the legitimate daughters of the Spanish King Alfonso X “The Wise”. The genetic analysis of External Visible Characteristics in the mummified remains attributed to this Princess has allowed determining her origin by mitochondrial and nuclear DNA analysis, and her physical appearance for hair, eyes, and skin color by autosomal SNPs. The results show that the mummified remains correspond to a young European woman with black hair, green-hazel eyes, and white skin. Her physical appearance has not been possible to be compared with any pictorial source, but the biogeographical analysis results are consistent with the historiographic genealogical information. PB MDPI YR 2023 FD 2023-04-20 LK https://hdl.handle.net/20.500.14352/104862 UL https://hdl.handle.net/20.500.14352/104862 LA eng NO Palomo-Díez S, Gomes C, Fondevila M, Esparza-Arroyo Á, López-Parra A, Lareu M, Arroyo-Pardo E, Pastor J. Genetics Unveil the Genealogical Ancestry and Physical Appearance of an Unknown Historical Figure: Lady Leonor of Castile (Spain) (1256–1275). Genealogy. 2023;7(2):28 NO 2023 Descuento MDPI DS Docta Complutense RD 7 abr 2025