RT Journal Article T1 Application of Ligilactobacillus salivarius SP36, a Strain Isolated from an Old Cheese Seal, as an Adjunct Culture in Cheesemaking A1 Arias, Ramón A1 Alba Rubio, Claudio A1 Calzada, Javier A1 Jiménez, Lorena A1 Fernández, Leónides A1 Ávila, Marta A1 Román, Mario A1 Román, Juan A1 Rodríguez Gómez, Juan Miguel A1 Garde, Sonia AB Adjunct cultures originating from artisanal cheese environments may play an important role in recreating and developing traditional cheese flavours, thanks to their enzymatic activities, involved in different metabolic pathways that occur during cheese ripening. In this work, Ligilactobacillus salivarius SP36, a strain isolated from an old cheese seal, was added as an adjunct culture to the cheese’s raw milk, and its effect on the microbiological, physical–chemical and sensory characteristics of the cheese was studied. The use of L. salivarius SP36 in cheese manufacturing had no significant (p > 0.05) effect on the cheese microbiota, gross composition (fat percentage, protein, total solids, moisture and NaCl concentration), colour or texture of the cheese. However, L. salivarius SP36 increased (p < 0.01) the formation of 25 volatile compounds, including 10 esters, 1 aldehyde, 8 alcohols and 6 carboxylic acids. In addition, cheeses made with L. salivarius SP36 received higher scores (p < 0.01) for aroma intensity and quality than control cheeses. L. salivarius SP36 proved to be a good candidate as an adjunct culture for cheesemaking, since it improved the cheese flavour by making it more intense and recovering typical sensorial notes of traditional cheeses. PB MDPI YR 2024 FD 2024-07-21 LK https://hdl.handle.net/20.500.14352/107720 UL https://hdl.handle.net/20.500.14352/107720 LA eng NO Arias, R.; Alba, C.; Calzada, J.; Jiménez, L.; Fernández, L.; Ávila, M.; Roman, M.; Roman, J.; Rodríguez, J.M.; Garde, S. Application of Ligilactobacillus salivarius SP36, a Strain Isolated from an Old Cheese Seal, as an Adjunct Culture in Cheesemaking. Foods 2024, 13, 2296. https://doi.org/ 10.3390/foods13142296 DS Docta Complutense RD 10 abr 2025