RT Journal Article
T1 Feasibility and application of liquid-liquid extraction combined with gas chromatography-mass spectrometry for the analysis of phenolic acids from polyphenols degraded by human faecal microbiota
A1 Muñoz-González, Carolina
A1 Moreno-Arribas, Maria Victoria
A1 Rodríguez Bencomo, Juan José
A1 Cueva, Carolina
A1 Martín-Álvarez, Pedro Jesús
A1 Bartolomé, Begoña
A1 Pozo-Bayón, Maria Angeles
AB In this study the feasibility of a LLE-GC-EI-MS method for the analysis of 43 phenolic acids belonging to different chemical structure families which have been described in the literature as microbial-derived metabolites after consumption of dietary polyphenols was proved. In addition, the method was applied for the characterisation of phenolic metabolites resulting from the incubation, in anaerobic conditions, of a commercial grape seed extract (GSE) and their corresponding flavan-3-ol monomeric (GSE-M) and oligomeric (GSE-O) fractions with human faeces from healthy volunteers (n = 3). The method showed average values of repeatability and reproducibility of 5.0% and 6.3%, respectively, adequate and low detection (1.8-30.8 μg L -1) and quantification limits (6.0-102.8 μg L -1) and good recovery values (95%, as average value). A total of 27 phenolic acids were identified in the faecal solutions after incubation with the grape seed extracts. In general, faecal samples incubated with GSE and GSE-M (monomeric fraction) yield a higher formation of phenolic acids compared to the samples incubated with the oligomer fraction (GSE-O).
PB Elsevier
SN 0308-8146
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/93388
UL https://hdl.handle.net/20.500.14352/93388
LA eng
NO Muñoz-González, C., et al. «Feasibility and Application of Liquid–Liquid Extraction Combined with Gas Chromatography–Mass Spectrometry for the Analysis of Phenolic Acids from Grape Polyphenols Degraded by Human Faecal Microbiota». Food Chemistry, vol. 133, n.o 2, julio de 2012, pp. 526-35. https://doi.org/10.1016/j.foodchem.2012.01.020.
NO Ministerio de Ciencia e Innovación (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 20 abr 2025