RT Journal Article
T1 Analysis of Human Natural Killer Cell Metabolism
A1 Traba, Javier
A1 Waldmann, Thomas
A1 Antón Hurtado, Olga María
AB Natural Killer (NK) cells mediate mainly innate anti-tumor and anti-viral immune responses and respond to a variety of cytokines and other stimuli to promote survival, cellular proliferation, production of cytokines such as interferon gamma (IFNγ) and/or cytotoxicity programs. NK cell activation by cytokine stimulation requires a substantial remodeling of metabolic pathways to support their bioenergetic and biosynthetic requirements. There is a large body of evidence that suggests that impaired NK cell metabolism is associated with a number of chronic diseases including obesity and cancer, which highlights the clinical importance of the availability of a method to determine NK cell metabolism. Here we describe the use of an extracellular flux analyzer, a platform that allows real-time measurements of glycolysis and mitochondrial oxygen consumption, as a tool to monitor changes in the energy metabolism of human NK cells. The method described here also allows for the monitoring of metabolic changes after stimulation of NK cells with cytokines such as IL-15, a system that is currently being investigated in a wide range of clinical trials.
PB My Jove Corporation
SN 1940-087X
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/96932
UL https://hdl.handle.net/20.500.14352/96932
LA eng
NO Traba, Javier, et al. «Analysis of Human Natural Killer Cell Metabolism». Journal of Visualized Experiments, n.o 160, junio de 2020, p. 61466. https://doi.org/10.3791/61466.
NO National Institute of Health (USA)
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 10 abr 2025