RT Journal Article
T1 Lipid nanoparticles as nano-Trojan-horses for siRNA delivery and gene-knockdown
A1 Sánchez Arribas, Natalia
A1 Velasco Rodríguez, Brenda
A1 Aicart Sospedra, Emilio
A1 Guerrero Martínez, Andrés
A1 Junquera González, María Elena
A1 Taboada, Pablo
AB The therapeutic messenger RNA strategies, such as those using small interfering RNAs, take several advantages (versatility, efficiency and selectivity) over plasmid DNA-based strategies. However, the challenge remains to find nanovectors capable of properly loading the genetic material, transporting it through troublesome environments, like a tumoral site, and delivering it into the cytoplasm of target cells. Here, lipid nanoparticles, consisting of a gemini cationic/neutral helper lipid mixture, are proposed as siRNA nanovector. Cells from cervical and brain cancer overexpressing the green fluorescent protein (GFP) were chosen to analyse the biological response as well as the efficiency and safety of the siRNA-loaded nanovector according to the cell phenotype. Flow cytometry and epifluorescence or confocal microscopy were used to follow the gene knockdown in these overexpressed cells. The effect of the nanovector on cellular proliferation was evaluated with cytotoxicity assays while their potential oxidative stress generation was determined by quantifying the generation of reactive oxygen species. To explore the mechanism of cellular uptake, different inhibitors of endocytic pathways were used during incubation with cells. Finally, nanovectors were incubated in 3D-grown cells (spheroids) to see whether they can penetrate the complex tumoral microenvironments, their efficiency to knockdown GFP expression being monitored by confocal microscopy.
PB Elsevier
SN 1095-7103
SN 0021-9797
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/110601
UL https://hdl.handle.net/20.500.14352/110601
LA eng
NO Sánchez-Arribas, Natalia, et al. «Lipid Nanoparticles as Nano-Trojan-Horses for siRNA Delivery and Gene-Knockdown». Journal of Colloid and Interface Science, vol. 679, febrero de 2025, pp. 975-87. DOI.org (Crossref), https://doi.org/10.1016/j.jcis.2024.10.115
NO Received 20 June 2024; Received in revised form 17 October 2024; Accepted 19 October 2024Available online 22 October 20240021-9797/© 2024 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license ( http://creativecommons.org/licenses/bync-nd/4.0/ ).NOTA IMPORTANTE: Esta publicación ha sido aceptada en Octubre de 2024 (https://doi.org/10.1016/j.jcis.2024.10.115), aunque la editorial la ha encajado en el Volumen 679, Part B, February 2925, con referencia Journal of Colloid and Interface Science 679 (2025) 975–987
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Xunta de Galicia
DS Docta Complutense
RD 10 abr 2025