%0 Journal Article
%A Muñoz Colmenero, Ana Marta
%A Martinez, José Luis
%A Roca, Agustín
%A García-Vázquez, Eva
%T NGS tools for traceability in candies as high processed food products: Ion Torrent PGM versus conventional PCR-cloning
%D 2017
%@ 0308-8146
%U https://hdl.handle.net/20.500.14352/92428
%X The Next Generation Sequencing methodologies are considered the next step within DNA-based methods and their applicability in different fields is being evaluated. Here, we tested the usefulness of the Ion Torrent Personal Genome Machine (PGM) in food traceability analyzing candies as a model of high processed foods, and compared the results with those obtained by PCR-cloning-sequencing (PCR-CS). The majority of samples exhibited consistency between methodologies, yielding more information and species per product from the PGM platform than PCR-CS. Significantly higher AT-content in sequences of the same species was also obtained from PGM. This together with some taxonomical discrepancies between methodologies suggest that the PGM platform is still pre-mature for its use in food traceability of complex highly processed products. It could be a good option for analysis of less complex food, saving time and cost per sample.
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