RT Journal Article
T1 NGS tools for traceability in candies as high processed food products: Ion Torrent PGM versus conventional PCR-cloning
A1 Muñoz Colmenero, Ana Marta
A1 Martinez, José Luis
A1 Roca, Agustín
A1 García-Vázquez, Eva
AB The Next Generation Sequencing methodologies are considered the next step within DNA-based methods and their applicability in different fields is being evaluated. Here, we tested the usefulness of the Ion Torrent Personal Genome Machine (PGM) in food traceability analyzing candies as a model of high processed foods, and compared the results with those obtained by PCR-cloning-sequencing (PCR-CS). The majority of samples exhibited consistency between methodologies, yielding more information and species per product from the PGM platform than PCR-CS. Significantly higher AT-content in sequences of the same species was also obtained from PGM. This together with some taxonomical discrepancies between methodologies suggest that the PGM platform is still pre-mature for its use in food traceability of complex highly processed products. It could be a good option for analysis of less complex food, saving time and cost per sample.
PB Elsevier
SN 0308-8146
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/92428
UL https://hdl.handle.net/20.500.14352/92428
LA eng
NO Muñoz-Colmenero, Marta, et al. «NGS Tools for Traceability in Candies as High Processed Food Products: Ion Torrent PGM versus Conventional PCR-Cloning». Food Chemistry, vol. 214, enero de 2017, pp. 631-36. https://doi.org/10.1016/j.foodchem.2016.07.121.
NO Ministerio de Universidades (España)
NO Principado de Asturias
NO Fundación Mafre
DS Docta Complutense
RD 8 abr 2025