RT Journal Article
T1 Nitric oxide from mononuclear cells may be involved in platelet responsiveness to aspirin
A1 López Farre, Antonio José
A1 Modrego Javier, 
A1 Azcona, Luis
A1 Guerra, Redy
A1 Segura, Antonio
A1 Rodríguez Sierra, Pablo
A1 Zamorano León, José Javier
A1 Lahera Julia, Vicente
A1 Macaya Miguel, Carlos
AB BackgroundSeveral mechanisms have been proposed to explain why some platelets have a reduced response to aspirin (ASA). Among them, it was reported an increased circulating level of vitamin-D-binding protein (DBP). In addition, nitric oxide (NO) released from mononuclear cells was involved in the antiplatelet effects of ASA. The aim was to analyse the relationship between platelet response to ASA and both NO generation and vitamin-D-binding protein content in mononuclear cells.Materials and methodsMononuclear cells were obtained from patients with stable coronary artery disease that were divided by a platelet functionality test (PFA-100) as ASA-sensitive (n = 23) and ASA resistant (n = 27).ResultsBoth the release of NO (determined by nitrite + nitrate concentration) and the expression of endothelial-type NO synthase (eNOS) were higher in mononuclear cells from ASA sensitive as compared with those from ASA-resistant patients. There was a positive correlation between either the release of NO and the expression of eNOS protein in mononuclear cells with the ability of ASA to inhibit platelet activity. DBP content in mononuclear cells was higher in ASA resistant than in ASA sensitive. The level of DBP content in mononuclear cells was negatively associated with the ability of ASA to inhibit platelets. However, in vitro experiments suggested that there was no association between DBP and NO production by mononuclear cells.ConclusionsMononuclear cells from patients with platelets with lower responsiveness to ASA showed a reduced ability to produce NO.
PB Wiley
SN 0014-2972
YR 2014
FD 2014-07-01
LK https://hdl.handle.net/20.500.14352/100148
UL https://hdl.handle.net/20.500.14352/100148
LA eng
NO López-Farré AJ, Modrego J, Azcona L, Guerra R, Segura A, Rodríguez P, Zamorano-León JJ, Lahera V, Macaya C. Nitric oxide from mononuclear cells may be involved in platelet responsiveness to aspirin. Eur J Clin Invest. 2014 May;44(5):463-9. doi: 10.1111/eci.12252
DS Docta Complutense
RD 17 abr 2025