RT Journal Article
T1 Fungal ribotoxins: molecular dissection of a familyof natural killers
A1 Lacadena García-Gallo, Francisco Javier
A1 Álvarez García, Elisa
A1 Carreras Sangrà, Nelson
A1 Herrero Galán, Elías
A1 Alegre Cebollada, Jorge
A1 García Ortega, Lucía
A1 Oñaderra, Mercedes
A1 Gavilanes, José G.
A1 Martínez Del Pozo, Álvaro
AB RNase T1 is the best known representative of a large family of ribonucleolyticproteins secreted by fungi, mostly Aspergillus and Penicillium species. Ribotoxinsstand out among them by their cytotoxic character. They exert their toxic action byfirst entering the cells and then cleaving a single phosphodiester bond locatedwithin a universally conserved sequence of the large rRNA gene, known as thesarcin–ricin loop. This cleavage leads to inhibition of protein biosynthesis,followed by cellular death by apoptosis. Although no protein receptor has beenfound for ribotoxins, they preferentially kill cells showing altered membranepermeability, such as those that are infected with virus or transformed. Many stepsof the cytotoxic process have been elucidated at the molecular level by means of avariety of methodological approaches and the construction and purification ofdifferent mutant versions of these ribotoxins. Ribotoxins have been used for theconstruction of immunotoxins, because of their cytotoxicity. Besides this activity,Aspf1, a ribotoxin produced by Aspergillus fumigatus, has been shown to be one ofthe major allergens involved in allergic aspergillosis-related pathologies. Proteinengineering and peptide synthesis have been used in order to understand the basisof these pathogenic mechanisms as well as to produce hypoallergenic proteins withpotential diagnostic and immunotherapeutic applications.
SN 0168-6445
YR 2007
FD 2007
LK https://hdl.handle.net/20.500.14352/52715
UL https://hdl.handle.net/20.500.14352/52715
LA spa
DS Docta Complutense
RD 6 abr 2025