%0 Journal Article
%A Villegas, María Fernanda
%A García Uriostegui, Lorena
%A Rodríguez, Ofelia
%A Izquierdo Barba, Isabel
%A Salinas Sánchez, Antonio Jesús
%A Toriz, Guillermo
%A Vallet Regí, María Dulce Nombre
%A Delgado, Ezequiel
%T Lysine-Grafted MCM-41 Silica as An Antibacterial Biomaterial
%D 2017
%@ 2306-5354; CODEN: BIOEBG
%U https://hdl.handle.net/20.500.14352/18120
%X Abstract: A facile strategy for zwitterionization of bioceramics based on direct incorporation of L-lysine amino acid via the ε-amino group onto mesoporous MCM-41 materials is proposed. FTIR studies of lysine-grafted MCM-41 (MCM-LYS) showed simultaneously bands at 3080 and 1540 cm−1 and bands at 1625 and 1415 cm−1 corresponding to -NH3+/COO− pairs, demonstrating the incorporation of the amino acid on the material surface keeping its zwitterionic character. Both elemental and thermogravimetric analyses showed that the amount of grafted lysine was 8 wt % based on the bioceramic total weight. Moreover, MCM-LYS material exhibited a reduction of adhesion of S. aureus and E. coli bacteria in 33 and 50%, respectively at physiological pH, as compared with pristine MCM-41. Biofilm studies onto surfaces showed that lysine functionalization elicited a reduction of the area covered by S. aureus biofilm from 42% to only 5% (88%). This research shows a simple and effective approach to chemically modify bioceramics using single amino acids that provide zwitterionic functionality, useful to develop new biomaterials able to resist bacterial adhesion.
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